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04.12.2015 | Ultrasound

Effects of diagnostic ultrasound-targeted microbubble destruction on the homing ability of bone marrow stromal cells to the kidney parenchyma

verfasst von: Gong Wang, Qian Zhang, Zhongxiong Zhuo, Shengzheng Wu, Zheng Liu, Hongmei Xia, Kaibin Tan, Linru Zou, Ling Gan, Yunhua Gao

Erschienen in: European Radiology | Ausgabe 9/2016

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Abstract

Objectives

Bone marrow stromal cells (BMSC) transplantation proves successful in treating kidney disease and injury in many studies. However, their reparative capacity is limited by the poor homing ability in vivo, which is decided mainly by the local expression of chemoattractants. Our study explored the mechanical effects of ultrasound targeted microbubble destruction (UTMD) on BMSCs homing ability in treated kidney tissues.

Methods

Rats were injected with red fluorescent protein (RFP)-labelled BMSCs and sonicated with microbubble-mediated ultrasound. Then, we tested kidney micro-environment changes induced and their influence on stem cell homing ability.

Results

The results showed that the mechanical effects of UTMD would increase local and transient levels of chemoattractants (i.e. cytokines, integrins and growth factors) in targeted kidney tissues. Transmission electron microscopy showed that vascular endothelial cell was discontinuous in the UTMD group post-treatment, becoming smooth 72 h later. Confocal laser scanning microscopy and RT-PCR showed up to eight times more stem cells in the peritubular regions of experimental kidneys on days 1 and 3 post-treatment compared with the contralateral kidney.

Conclusions

These results confirmed that renal micro-environment changes caused by appropriate UTMD may promote BMSC homing ability toward treated kidney tissues without renal toxicity and cell damage.

Key Points

• This experiment showed a feasible strategy in promoting stem cell homing ability.

• The treatment uses diagnostic ultrasound during enhancement with IV microbubbles.

• A suitable micro-environment was important for targeted stem cell homing and retention.

• The method is effective for stem cell homing to kidney diseases.

• More work is required with larger animals before potential human trials.

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Titel: Effects of diagnostic ultrasound-targeted microbubble destruction on the homing ability of bone marrow stromal cells to the kidney parenchyma
verfasst von: Gong Wang
Qian Zhang
Zhongxiong Zhuo
Shengzheng Wu
Zheng Liu
Hongmei Xia
Kaibin Tan
Linru Zou
Ling Gan
Yunhua Gao
Publikationsdatum: 04.12.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: European Radiology / Ausgabe 9/2016
Print ISSN: 0938-7994
Elektronische ISSN: 1432-1084
DOI: https://doi.org/10.1007/s00330-015-4123-3

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Effects of diagnostic ultrasound-targeted microbubble destruction on the homing ability of bone marrow stromal cells to the kidney parenchyma

Abstract

Objectives

Methods

Results

Conclusions

Key Points

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Springer Medizin

Abstract

Objectives

Methods

Results

Conclusions

Key Points

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