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Erschienen in: Comparative Clinical Pathology 4/2019

23.11.2018 | Original Article

Effects of different doses of curcumin on testicular histopathology, apoptosis, and reproductive organs weight index in mice d-galactose-induced aging model

verfasst von: Mahdieh Yousefi Taba, Shabnam Mohammadi, Mehdi Jalali, Farimah Beheshti, Seyedeh Saleheh Attari

Erschienen in: Comparative Clinical Pathology | Ausgabe 4/2019

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Abstract

Due to the value of aging and its effect on body organs, it is necessary to study the factors that can have protective effects on this process. Curcumin is the active ingredient of Curcuma longa Turmeric, which has a strong antioxidant effect. We evaluated the curcumin treatment on testicular histopathology, apoptosis, and reproductive organs index in a mouse model of aging. Forty-eight mice divided into control, sham, d-galactose, and curcumin 1 to 3 groups. The d-galactose group was administered intraperitoneally 300 mg/kg d-galactose for 6 weeks. The curcumin 1 to 3 groups received d-galactose plus 25, 50, and 100 mg/kg of curcumin, respectively for 14 days. Then, testicular histopathology, apoptosis, and reproductive organs index were investigated after 6 weeks. The SPSS software was used for data analysis. Tunnel-positive cells in the d-galactose group were remarkably higher than the control group (p < 0.05). Administration of curcumin in all three doses reduced the number of positive tunnel cells, in comparison with the group that received d-galactose (p < 0.05). The epididymis weight index of the d-galactose group was significantly different from the control (p = 0.006), the sham (p = 0.01), the curcumin 1 (p = 0.003), the curcumin 2 (p = 0.003), and the curcumin 3 (p = 0.002) groups. Histological examination of the d-galactose group revealed a reduction in the thickness of the germinal epithelium, edema, and complete atrophy of germ cells in some seminiferous tubules. The histology of seminiferous tubules was near-normal appearance with the little disintegration of germ cells from their normal germinal epithelium in the curcumin 1 and 3 groups. The histology of seminiferous tubules was near-normal appearance with mild edema and congestion in some seminiferous tubules in the curcumin 2 group. Curcumin supplementation (2 weeks, 100 mg/kg) caused an improvement in biochemical markers and sperm parameters as well as a reduction in apoptosis in the testicular tissue of the aging mouse model.
Literatur
Zurück zum Zitat Attari S, Mohammadi S, Ebrahimzadeh A, Hosseinzadeh H, Soukhtanloo M, Rajabzadeh A (2018) Effects of thymoquinone on sperm parameters, apoptosis, testosterone level, and oxidative stress in a mouse model of D-galactose-induced aging. Pharm Sci. 24 (2):180-186. Attari S, Mohammadi S, Ebrahimzadeh A, Hosseinzadeh H, Soukhtanloo M, Rajabzadeh A (2018) Effects of thymoquinone on sperm parameters, apoptosis, testosterone level, and oxidative stress in a mouse model of D-galactose-induced aging. Pharm Sci. 24 (2):180-186. 
Zurück zum Zitat Karbalay-Doust S, Noorafshan A (2011) Ameliorative effects of curcumin on the spermatozoon tail length, count, motility and testosterone serum level in metronidazole-treated mice. Prague Med Rep 112(4):288–297PubMed Karbalay-Doust S, Noorafshan A (2011) Ameliorative effects of curcumin on the spermatozoon tail length, count, motility and testosterone serum level in metronidazole-treated mice. Prague Med Rep 112(4):288–297PubMed
Zurück zum Zitat Mohammadi S, Khakbaz M, Shoraka M, Vakil S, Moghimian M, Mohammadzadeh F (2016a) Effects of different doses of manganese on lead poisoning in the kidney of adult male mice. Koomesh 18(1):203–210 Mohammadi S, Khakbaz M, Shoraka M, Vakil S, Moghimian M, Mohammadzadeh F (2016a) Effects of different doses of manganese on lead poisoning in the kidney of adult male mice. Koomesh 18(1):203–210
Zurück zum Zitat Mohammadi S, Safari F, Seyedi Z, Seyed Hosseini E, Karimi F, Mohammadi M, Karimi M, Delshad A, Abtahi H, Mohammadzadeh F (2016b) Effect of different doses of N-acetyl cysteine on biochemical and histopathological parameters in kidney of formalin-treated. JKMU 3(5):607–617 Mohammadi S, Safari F, Seyedi Z, Seyed Hosseini E, Karimi F, Mohammadi M, Karimi M, Delshad A, Abtahi H, Mohammadzadeh F (2016b) Effect of different doses of N-acetyl cysteine on biochemical and histopathological parameters in kidney of formalin-treated. JKMU 3(5):607–617
Zurück zum Zitat Mohammadi S, Golamin M, Mohammadi M, Mansouri A, Mahmoodian R, Attari S (2018a) Down-regulation of CatSper 1 and CatSper 2 genes by lead and mercury. Environ Toxicol Pharmacol 59:82–86CrossRefPubMed Mohammadi S, Golamin M, Mohammadi M, Mansouri A, Mahmoodian R, Attari S (2018a) Down-regulation of CatSper 1 and CatSper 2 genes by lead and mercury. Environ Toxicol Pharmacol 59:82–86CrossRefPubMed
Zurück zum Zitat Mohammadi S, Golamin M, Mohammadi M, Mansouri A, Mahmoodian R, Attari S, Kebriaei SM, Zibaei B, Roshanaei M, Daneshvar F, Khandehro M, Khodadadegan MA, Delshad A, Mohammadzadeh F, Peyvandi M, Ghayour-Mobarhan M, Tavallaie S, Boroumand-Noughabi S, Ferns AAG (2018b) Down-regulation of CatSper 1 and CatSper 2 genes by lead and mercury. Environ Toxicol Pharmacol 59:82–86CrossRefPubMed Mohammadi S, Golamin M, Mohammadi M, Mansouri A, Mahmoodian R, Attari S, Kebriaei SM, Zibaei B, Roshanaei M, Daneshvar F, Khandehro M, Khodadadegan MA, Delshad A, Mohammadzadeh F, Peyvandi M, Ghayour-Mobarhan M, Tavallaie S, Boroumand-Noughabi S, Ferns AAG (2018b) Down-regulation of CatSper 1 and CatSper 2 genes by lead and mercury. Environ Toxicol Pharmacol 59:82–86CrossRefPubMed
Zurück zum Zitat Momtaz S, Abdollahi M (2012) A comprehensive review of biochemical and molecular evidences from animal and human studies on the role of oxidative stress in aging: an epiphenomenon or the cause. Asian J Anim Vet Adv 7:1–19CrossRef Momtaz S, Abdollahi M (2012) A comprehensive review of biochemical and molecular evidences from animal and human studies on the role of oxidative stress in aging: an epiphenomenon or the cause. Asian J Anim Vet Adv 7:1–19CrossRef
Zurück zum Zitat Shen LR, Parnell LD, Ordovas JM, Lai CQ (2013a) Curcumin and aging. Biofactors 39(1):133–140CrossRefPubMed Shen LR, Parnell LD, Ordovas JM, Lai CQ (2013a) Curcumin and aging. Biofactors 39(1):133–140CrossRefPubMed
Zurück zum Zitat Singh S, Das Roy L, Giri S (2013) Curcumin protects metronidazole and X-ray induced cytotoxicity and oxidative stress in male germ cells in mice. Prague Med Rep 114(2):92–102CrossRefPubMed Singh S, Das Roy L, Giri S (2013) Curcumin protects metronidazole and X-ray induced cytotoxicity and oxidative stress in male germ cells in mice. Prague Med Rep 114(2):92–102CrossRefPubMed
Zurück zum Zitat Tajaddini S, Ebrahimi S, Shirinbayan P, Bakhtiari M, Behnam B, Joghataei MT (2013) Protective effects of manganese on the testis structure and sperm parameters of formalin treated mice. J Isfahan Med Sch 31(243):1018–1032 Tajaddini S, Ebrahimi S, Shirinbayan P, Bakhtiari M, Behnam B, Joghataei MT (2013) Protective effects of manganese on the testis structure and sperm parameters of formalin treated mice. J Isfahan Med Sch 31(243):1018–1032
Zurück zum Zitat Wang W, Li S, Dong H-P, Lv S, Tang Y-Y (2009) Differential impairment of spatial and nonspatial cognition in a mouse model of brain aging. Life Sci 85(3):127–135CrossRefPubMed Wang W, Li S, Dong H-P, Lv S, Tang Y-Y (2009) Differential impairment of spatial and nonspatial cognition in a mouse model of brain aging. Life Sci 85(3):127–135CrossRefPubMed
Metadaten
Titel
Effects of different doses of curcumin on testicular histopathology, apoptosis, and reproductive organs weight index in mice d-galactose-induced aging model
verfasst von
Mahdieh Yousefi Taba
Shabnam Mohammadi
Mehdi Jalali
Farimah Beheshti
Seyedeh Saleheh Attari
Publikationsdatum
23.11.2018
Verlag
Springer London
Erschienen in
Comparative Clinical Pathology / Ausgabe 4/2019
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-018-2870-7

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