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Erschienen in: Osteoporosis International 5/2011

01.05.2011 | Original Article

Effects of HIV infection and antiretroviral therapy with ritonavir on induction of osteoclast-like cells in postmenopausal women

verfasst von: M. T. Yin, R. Modarresi, E. Shane, F. Santiago, D. C. Ferris, D. J. McMahon, C. A. Zhang, S. Cremers, J. Laurence

Erschienen in: Osteoporosis International | Ausgabe 5/2011

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Abstract

Summary

Ritonavir (RTV) is a commonly used antiretroviral associated with bone loss. We show that peripheral blood mononuclear cells (PBMCs) from human immunodeficiency virus (HIV)-positive women on RTV are more likely to differentiate into osteoclast-like cells when cultured with their own sera than PBMCs and sera from HIV− women or HIV+ on other antiretrovirals.

Introduction

RTV increases differentiation of human adherent PBMCs to functional osteoclasts in vitro, and antiretroviral regimens containing RTV have been associated with low bone mineral density (BMD) and bone loss.

Methods

BMD, proresorptive cytokines, bone turnover markers (BTMs), and induction of osteoclast-like cells from adherent PBMCs incubated either with macrophage colony-stimulating factor (MCSF) and receptor activator of nuclear factor κB ligand (RANKL) or with autologous serum were compared in 51 HIV− and 68 HIV+ postmenopausal women.

Results

BMD was lower, and serum proresorptive cytokines and BTMs were higher in HIV+ versus HIV− women. Differentiation of osteoclast-like cells from adherent PBMCs exposed to either MCSF/RANKL or autologous serum was greater in HIV+ women. Induction of osteoclast-like cells was greater from PBMCs exposed to autologous sera from HIV+ women on RTV-containing versus other regimens (172 ± 14% versus 110 ± 10%, p < 0.001). Serum-based induction of osteoclast-like cells from adherent PBMCs correlated with certain BTMs but not BMD.

Conclusions

HIV infection and antiretroviral therapy are associated with higher BTMs and increased differentiation of osteoclast-like cells from adherent PBMCs, especially in women on regimens containing RTV. HIV+ postmenopausal women receiving RTV may be at greater risk for bone loss.
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Metadaten
Titel
Effects of HIV infection and antiretroviral therapy with ritonavir on induction of osteoclast-like cells in postmenopausal women
verfasst von
M. T. Yin
R. Modarresi
E. Shane
F. Santiago
D. C. Ferris
D. J. McMahon
C. A. Zhang
S. Cremers
J. Laurence
Publikationsdatum
01.05.2011
Verlag
Springer-Verlag
Erschienen in
Osteoporosis International / Ausgabe 5/2011
Print ISSN: 0937-941X
Elektronische ISSN: 1433-2965
DOI
https://doi.org/10.1007/s00198-010-1363-6

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