Communicated by Fabio Fischetti.
To assess evidence of health and immune benefit by consumption of a Lactobacillus casei Shirota probiotic in highly physically active people.
Single-centre, population-based, randomized, double-blind, placebo-controlled trial. Daily ingestion of probiotic (PRO) or placebo (PLA) for 20 weeks for n = 243 (126 PRO, 117 PLA) university athletes and games players. Subjects completed validated questionnaires on upper respiratory tract infection symptoms (URS) on a daily basis and on physical activity status at weekly intervals during the intervention period. Blood samples were collected before and after 20 weeks of the intervention for determination of Epstein Barr virus (EBV) and cytomegalovirus (CMV) serostatus and antibody levels.
URS episode incidence was unexpectedly low (mean 0.6 per individual) and was not significantly different on PRO compared with PLA. URS episode duration and severity were also not influenced by PRO. A significant time × group interaction effect was observed for plasma CMV antibody titres in CMV seropositive participants (p < 0.01) with antibody titre falling in the PRO group but remaining unchanged in the PLA group over time. A similar effect was found for plasma EBV antibody titres in EBV seropositive participants (p < 0.01) with antibody titre falling in the PRO group but increasing in the PLA group over time.
In summary, regular ingestion of PRO did not reduce URS episode incidence which might be attributable to the low URS incidence in this study. Regular ingestion of PRO reduced plasma CMV and EBV antibody titres, an effect that can be interpreted as a benefit to overall immune status.
Glaser R, Friedman SB, Smyth J, Ader R, Bijur P, Brunell P, Cohen N, Krilov LR, Lifrak ST, Stone A, Toffler P (1999) The differential impact of training stress and final examination stress on herpesvirus latency at the United States Military Academy at West Point. Brain Behav Immun 13(3):240–251 CrossRefPubMed
Gleeson M (2016) Immunological aspects of sport nutrition. Immunol Cell Biol 4(2):117–123 CrossRef
Gleeson M, Bishop NC, Oliveira M, Tauler PJ (2011) Daily probiotic’s ( Lactobacillus casei Shirota) reduction of infection incidence in athletes. Int J Sport Nutr Exerc Metab 21:55–64 PubMed
Gleeson M, Bishop NC, Oliveira M, McCauley T, Tauler P, Lawrence C (2012) Effects of a Lactobacillus salivarius probiotic intervention on infection, cold symptom duration and severity, and mucosal immunity in endurance athletes. Int J Sport Nutr Exerc Metab 22(4):235–242 PubMed
Gleeson M, Bishop NC, Walsh NP (2013b) Exercise immunology. Routledge, London
Hadrup SR, Strindhall J, Køllgaard T, Seremet T, Johansson B, Pawelec G, thor Straten P, Wikby A (2006) Longitudinal studies of clonally expanded CD8 T cells reveal a repertoire shrinkage predicting mortality and an increased number of dysfunctional cytomegalovirus-specific T cells in the very elderly. J Immunol 176(4):2645–2653 CrossRefPubMed
Hao Q, Dong BR, Wu T (2015) Probiotics for preventing acute upper respiratory tract infections. Cochrane Database Syst Rev 2:CD006895
Jackson GG, Dowling HF, Spiesman IG, Boano AV (1958) Transmission of the common cold to volunteers under controlled conditions. Arch Intern Med 101:267–278 CrossRef
Kekkonen RA, Vasankari TJ, Vuorimaa T, Haahtela T, Julkunen I, Korpela R (2007) The effect of probiotics on respiratory infections and gastrointestinal symptoms during training in marathon runners. Int J Sport Nutr Exerc Metab 17:352–383 PubMed
Marinkovic D, Minic R, Dikic N, Andjelkovic M, Kostic-Vucicevic M, Stojmenovic T, Nikolic I, Djordjevic B (2016a) Lactobacillus helveticus Lafti ® L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized double-blind placebo-controlled trial. J Appl Physiol Nutr Metab 2016a (in press)
Marinkovic D, Kostic-Vucicevic D, Vukasinovic-Vesic M, Stojmenovic T, Dikic N, Djordjevic B, Tanaskovic B, Rajna Minic L (2016b) helveticus Lafti ® L10 supplementation modulates mucosal and humoral immunity in elite athletes (in a randomized double-blinded placebo-controlled trial). J Strength Cond Res (in press)
Matsumoto K, Takada T, Shimizu K, Kado Y, Kawakami K, Makino I, Yamaoka Y, Hirano K, Nishimura A, Kajimoto O, Nomoto K (2006) The effects of a probiotic milk product containing Lactobacillus casei strain Shirota on the defecation frequency and the intestinal microflora of sub-optimal health state volunteers: a randomized placebo-controlled cross-over study. Bioscience Microflora 25(2):39–48 CrossRef
Moro-García MA, Alonso-Arias R, Baltadjieva M, Fernández Benítez C, Fernández Barrial MA, Díaz Ruisánchez E, Alonso Santos R, Alvarez Sánchez M, Saavedra Miján J, López-Larrea C (2013) Oral supplementation with Lactobacillus delbrueckii subsp. bulgaricus 8481 enhances systemic immunity in elderly subjects. Age 35(4):1311–13263 CrossRefPubMed
Nagao F, Nakayama M, Muto T, Okumura K (2000) Effects of a fermented milk drink containing Lactobacillus casei strain Shirota on the immune system in healthy human subjects. Biosci Biotech Biochem 64(12):2706–2708 CrossRef
Nieman DC, Johanssen LM, Lee IW, Arabatzis K (1990) Infectious episodes in runners before and after the Los Angeles Marathon. J Sports Med Phys Fitness 30(3):316–328 PubMed
Predy GN, Goel V, Lovlin R, Donner A, Stitt L, Basu TK (2005) Efficacy of an extract of North American ginseng containing poly-furanosyl-pyranosyl-saccharides for preventing upper respiratory tract infections: a randomized controlled trial. Can Med Assoc J 173(9):1043–1048 CrossRef
Public Health England National Weekly Influenza Report (2015) https://www.gov.uk/government/uploads/system/uploads/attachment_data/file/445791/Weekly_report_summary_wk29_16July2015.pdf
Simpson RJ, Bigley AB, Spielmann G, LaVoy EC, Kunz H, Bollard CM (2016) Human cytomegalovirus infection and the immune response to exercise. Exerc Immunol Rev 22:8–27 PubMed
Trejo F, Sanz Y (2013) Intestinal bacteria and probiotics: effects on the immune system and impacts on human health. In: Calder PC, Yaqoob P (eds) Diet, immunity and inflammation. Woodhead Publishing, Cambridge, UK, pp 267–291 CrossRef
Walsh NP, Gleeson M, Shephard RJ, Gleeson M, Woods JA, Bishop NC, Fleshner M, Green C, Pedersen BK, Hoffman-Goetz L, Rogers CJ, Northoff H, Abbasi A, Simon P (2011a) Position statement part one: immune function and exercise. Exerc Immunol Rev 17:6–63 PubMed
Walsh NP, Gleeson M, Pyne DB, Nieman DC, Dhabhar FS, Shephard RJ, Oliver SJ, Bermon S, Kajeniene A (2011b) Position statement part two: maintaining immune health. Exerc Immunol Rev 17:64–103 PubMed
West NP, Pyne DB, Cripps AW, Hopkins WG, Eskesen DC, Jairath A, Christophersen CT, Conlon MA, Fricker PA (2011) Lactobaccillus fermentum (PCC ®) supplementation and gastrointestinal and respiratory tract illness symptoms: a randomised control trial in athletes. Nutr J 10:30 CrossRefPubMedPubMedCentral
- Effects of Lactobacillus casei Shirota ingestion on common cold infection and herpes virus antibodies in endurance athletes: a placebo-controlled, randomized trial
Nicolette C. Bishop
- Springer Berlin Heidelberg