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Erschienen in: Lasers in Medical Science 1/2017

27.09.2016 | Original Article

Effects of low-level laser therapy and epidermal growth factor on the activities of gingival fibroblasts obtained from young or elderly individuals

verfasst von: Taisa Nogueira Pansani, Fernanda Gonçalves Basso, Ana Paula Silveira Turrioni, Diana Gabriela Soares, Josimeri Hebling, Carlos Alberto de Souza Costa

Erschienen in: Lasers in Medical Science | Ausgabe 1/2017

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Abstract

This study evaluated the effects of low-level laser therapy (LLLT) and epidermal growth factor (EGF) on fibroblasts obtained from young and elderly individuals. Gingival fibroblasts from young (Y) and elderly (E) individuals were seeded in wells of 24-well plates with Dulbecco’s modified Eagle’s medium (DMEM) containing 10 % of fetal bovine serum (FBS). After 24 h, the cells were irradiated (LASERTable—InGaAsP—780 ± 3 nm, 25 mW, 3 J/cm2) or exposed to EGF (100 μM). After 72 h, cells were evaluated for viability, migration, collagen and vascular endothelial growth factor (VEGF) synthesis, and gene expression of growth factors. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (α = 5 %). Y and E fibroblasts irradiated with laser or exposed to EGF showed increased viability and collagen synthesis. Enhanced cell migration was observed for Y fibroblasts after both treatments, whereas only the LLLT stimulated migration of E cells. VEGF synthesis was higher for Y and E cells exposed to EGF, while this synthesis was reduced when E fibroblasts were irradiated. Increased gene expression of VEGF was observed only for Y and E fibroblasts treated with LLLT. Regardless of a patient’s age, the LLLT and EGF applications can biostimulate gingival fibroblast functions involved in tissue repair.
Literatur
1.
Zurück zum Zitat Magalhães JP (2004) From cells to ageing: a review of models and mechanisms of cellular senescence and their impact on human ageing. Exp Cell Res 300:1–10CrossRefPubMed Magalhães JP (2004) From cells to ageing: a review of models and mechanisms of cellular senescence and their impact on human ageing. Exp Cell Res 300:1–10CrossRefPubMed
3.
Zurück zum Zitat Schulze C, Wetzel F, Kueper T, Malsen A, Muhr G, Jaspers S, Blatt T, Wittern KP, Wenck H, Käs JA (2010) Stiffening of human skin fibroblasts with age. Biophysical J 99:2434–2442CrossRef Schulze C, Wetzel F, Kueper T, Malsen A, Muhr G, Jaspers S, Blatt T, Wittern KP, Wenck H, Käs JA (2010) Stiffening of human skin fibroblasts with age. Biophysical J 99:2434–2442CrossRef
4.
Zurück zum Zitat Cristofalo VJ, Lorenzini A, Allen RG, Torres C, Tresini M (2004) Replicative senescence: a critical review. Mech Ageing Dev 125:827–848CrossRefPubMed Cristofalo VJ, Lorenzini A, Allen RG, Torres C, Tresini M (2004) Replicative senescence: a critical review. Mech Ageing Dev 125:827–848CrossRefPubMed
5.
Zurück zum Zitat Hwang ES, Gyesoon Y, Kang HT (2009) A comparative analysis of the cell biology of senescence and aging. Cell Mol Life Sci 66:2503–2524CrossRefPubMed Hwang ES, Gyesoon Y, Kang HT (2009) A comparative analysis of the cell biology of senescence and aging. Cell Mol Life Sci 66:2503–2524CrossRefPubMed
7.
Zurück zum Zitat Cochran DL, Wozney JM (1999) Biological mediators for periodontal regeneration. Periodontol 19:40–58CrossRef Cochran DL, Wozney JM (1999) Biological mediators for periodontal regeneration. Periodontol 19:40–58CrossRef
8.
Zurück zum Zitat Stephens P, Davies KJ, Al-Khateeb T, Shepherd JP, Thomas DW (1996) A comparison of the ability of intra-oral and extra-oral fibroblasts to stimulate extracellular matrix reorganization in a model of wound contraction. J Dent Res 75:1358–1364CrossRefPubMed Stephens P, Davies KJ, Al-Khateeb T, Shepherd JP, Thomas DW (1996) A comparison of the ability of intra-oral and extra-oral fibroblasts to stimulate extracellular matrix reorganization in a model of wound contraction. J Dent Res 75:1358–1364CrossRefPubMed
9.
Zurück zum Zitat Enoch S, Peake MA, Wall I, Davies L, Farrier J, Giles P, Kipling D, Price P, Moseley R, Thomas D, Stephens P (2010) ‘Young’ oral fibroblasts are geno/phenotypically distinct. J Dent Res 89:1407–1413CrossRefPubMed Enoch S, Peake MA, Wall I, Davies L, Farrier J, Giles P, Kipling D, Price P, Moseley R, Thomas D, Stephens P (2010) ‘Young’ oral fibroblasts are geno/phenotypically distinct. J Dent Res 89:1407–1413CrossRefPubMed
10.
Zurück zum Zitat Häkkinen L, Uitto VJ, Larjava H (2000) Cell biology of gingival wound healing. Periodontol 24:127–152CrossRef Häkkinen L, Uitto VJ, Larjava H (2000) Cell biology of gingival wound healing. Periodontol 24:127–152CrossRef
11.
Zurück zum Zitat Ohshima M, Sato M, Ishikawa M, Maeno M, Otsuka K (2002) Physiologic levels of epidermal growth factor in saliva stimulate cell migration of and oral epithelial cell line, HO-1-N-1. Eur J Oral Sci 100:130–136CrossRef Ohshima M, Sato M, Ishikawa M, Maeno M, Otsuka K (2002) Physiologic levels of epidermal growth factor in saliva stimulate cell migration of and oral epithelial cell line, HO-1-N-1. Eur J Oral Sci 100:130–136CrossRef
12.
Zurück zum Zitat Carpenter G, Cohen S (1990) Epidermal growth factor. J Biol Chem 265:7709–7712PubMed Carpenter G, Cohen S (1990) Epidermal growth factor. J Biol Chem 265:7709–7712PubMed
13.
Zurück zum Zitat Fujisawa K, Miyamoto Y, Nagayama M (2003) Basic fibroblast growth factor and epidermal growth factor reverse impaired ulcer healing of the rabbit oral mucosa. J Oral Pathol Med 32:358–366CrossRefPubMed Fujisawa K, Miyamoto Y, Nagayama M (2003) Basic fibroblast growth factor and epidermal growth factor reverse impaired ulcer healing of the rabbit oral mucosa. J Oral Pathol Med 32:358–366CrossRefPubMed
14.
Zurück zum Zitat Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M (2008) Growth factors and cytokines in wound healing. Wound Rep Reg 16:585–601CrossRef Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M (2008) Growth factors and cytokines in wound healing. Wound Rep Reg 16:585–601CrossRef
15.
Zurück zum Zitat Ryu S, Moon SY, Yang Y, Moon SR, Hong JP, Choi J, Lee SW (2009) Recombinant human epidermal growth factor accelerates the proliferation of irradiated human fibroblasts and keratinocytes in vitro and in vivo. J Radiat Res 50:545–552CrossRefPubMed Ryu S, Moon SY, Yang Y, Moon SR, Hong JP, Choi J, Lee SW (2009) Recombinant human epidermal growth factor accelerates the proliferation of irradiated human fibroblasts and keratinocytes in vitro and in vivo. J Radiat Res 50:545–552CrossRefPubMed
16.
Zurück zum Zitat Kong Q, Majeska RJ, Vazquez M (2011) Migration of connective tissue-derived cells is mediated by ultra-low concentration gradient fields of EGF. Exp Cell Res 317:1491–1502CrossRefPubMedPubMedCentral Kong Q, Majeska RJ, Vazquez M (2011) Migration of connective tissue-derived cells is mediated by ultra-low concentration gradient fields of EGF. Exp Cell Res 317:1491–1502CrossRefPubMedPubMedCentral
17.
Zurück zum Zitat Choi JK, Jang JH, Jang WH, Kim J, Bae IH, Bae J, Park YH, Kim BJ, Lim KM, Park JW (2012) The effect of epidermal growth factor (EGF) conjugated with low-molecular-weight protamine (LMWP) on wound healing of the skin. Biomaterials 33:8579–8590CrossRefPubMed Choi JK, Jang JH, Jang WH, Kim J, Bae IH, Bae J, Park YH, Kim BJ, Lim KM, Park JW (2012) The effect of epidermal growth factor (EGF) conjugated with low-molecular-weight protamine (LMWP) on wound healing of the skin. Biomaterials 33:8579–8590CrossRefPubMed
18.
Zurück zum Zitat Hardwicke J, Schmaljohann D, Boyce D, Thomas D (2008) Epidermal growth factor therapy and wound healing—past, present and future perspectives. Surgeon 6:172–177CrossRefPubMed Hardwicke J, Schmaljohann D, Boyce D, Thomas D (2008) Epidermal growth factor therapy and wound healing—past, present and future perspectives. Surgeon 6:172–177CrossRefPubMed
19.
Zurück zum Zitat Marques MM, Pereira NA, Fujihara NA, Nogueira FN, Eduardo CP (2004) Effect of low-power laser irradiation on protein synthesis and ultrastructure of human gingival fibroblasts. Lasers Surg Med 34:260–265CrossRefPubMed Marques MM, Pereira NA, Fujihara NA, Nogueira FN, Eduardo CP (2004) Effect of low-power laser irradiation on protein synthesis and ultrastructure of human gingival fibroblasts. Lasers Surg Med 34:260–265CrossRefPubMed
20.
Zurück zum Zitat Hawkins DH, Abrahamse H (2006) The role of lasers fluence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following helium-neon lasers irraditation. Lasers Surg Med 38:74–83CrossRefPubMed Hawkins DH, Abrahamse H (2006) The role of lasers fluence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following helium-neon lasers irraditation. Lasers Surg Med 38:74–83CrossRefPubMed
21.
Zurück zum Zitat Damante CA, De Micheli G, Miyagi SPH, Feist IS, Marques MM (2009) Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts. Lasers Med Sci 24:885–891CrossRefPubMed Damante CA, De Micheli G, Miyagi SPH, Feist IS, Marques MM (2009) Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts. Lasers Med Sci 24:885–891CrossRefPubMed
23.
Zurück zum Zitat Basso FG, Oliveira CF, Kurachi C, Hebling J, Costa CAS (2013) Biostimulatory effect of low-level laser therapy on keratinocytes in vitro. Lasers Med Sci 28:367–374CrossRefPubMed Basso FG, Oliveira CF, Kurachi C, Hebling J, Costa CAS (2013) Biostimulatory effect of low-level laser therapy on keratinocytes in vitro. Lasers Med Sci 28:367–374CrossRefPubMed
24.
Zurück zum Zitat Basso FG, Pansani TN, Soares DG, Scheffel DL, Bagnato VS, de Souza Costa CA, Hebling J (2015) Biomodulation of inflammatory cytokines related to oral mucositis by low-level laser therapy. Photochem Photobiol 91(4):952–956CrossRefPubMed Basso FG, Pansani TN, Soares DG, Scheffel DL, Bagnato VS, de Souza Costa CA, Hebling J (2015) Biomodulation of inflammatory cytokines related to oral mucositis by low-level laser therapy. Photochem Photobiol 91(4):952–956CrossRefPubMed
25.
Zurück zum Zitat Pansani TN, Basso FG, Turirioni AP, Kurachi C, Hebling J, de Souza Costa CA (2014) Effects of low-level laser therapy on the proliferation and apoptosis of gingival fibroblasts treated with zoledronic acid. Int J Oral Maxillofac Surg 43:1030–1034CrossRefPubMed Pansani TN, Basso FG, Turirioni AP, Kurachi C, Hebling J, de Souza Costa CA (2014) Effects of low-level laser therapy on the proliferation and apoptosis of gingival fibroblasts treated with zoledronic acid. Int J Oral Maxillofac Surg 43:1030–1034CrossRefPubMed
26.
Zurück zum Zitat Karu TI (2008) Michocondrial signaling in mammalian cells activated by red and near-IR radiation. Photochem Photobiol 84:1091–1099CrossRefPubMed Karu TI (2008) Michocondrial signaling in mammalian cells activated by red and near-IR radiation. Photochem Photobiol 84:1091–1099CrossRefPubMed
27.
Zurück zum Zitat Basso FG, Oliveira CF, Fontana F, Bagnato VS, Hebling J, de Souza Costa CA (2011) In vitro effect of low level laser therapy on typical oral microbial biofilms. Braz Dent J 5:502–510CrossRef Basso FG, Oliveira CF, Fontana F, Bagnato VS, Hebling J, de Souza Costa CA (2011) In vitro effect of low level laser therapy on typical oral microbial biofilms. Braz Dent J 5:502–510CrossRef
28.
Zurück zum Zitat Khadra M, Lyngstadaas SP, Haanæs HR, Mustafa K (2005) Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. Biomaterial 26:3503–3509CrossRef Khadra M, Lyngstadaas SP, Haanæs HR, Mustafa K (2005) Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. Biomaterial 26:3503–3509CrossRef
29.
Zurück zum Zitat Fujihara NA, Hiraki KRN, Marques MM (2006) Irradiation at 780 nm increases proliferation rate of osteoblasts independently of dexamethasone presence. Lasers Surg Med 38:332–336CrossRefPubMed Fujihara NA, Hiraki KRN, Marques MM (2006) Irradiation at 780 nm increases proliferation rate of osteoblasts independently of dexamethasone presence. Lasers Surg Med 38:332–336CrossRefPubMed
30.
Zurück zum Zitat Hoang AM, Oates TW, Cochran DL (2000) In vitro wound healing responses to enamel matrix derivative. J Periodontol 71:1270–1277CrossRefPubMed Hoang AM, Oates TW, Cochran DL (2000) In vitro wound healing responses to enamel matrix derivative. J Periodontol 71:1270–1277CrossRefPubMed
31.
Zurück zum Zitat Tullberg-Reinert H, Jundt G (1999) In situ measurement of collagen synthesis by human bone cells with a sirius red-based colorimetric microassay: effects of transforming growth factor beta 2 and ascorbic acid 2-phosphate. Histochem Cell Biol 112:271–276CrossRefPubMed Tullberg-Reinert H, Jundt G (1999) In situ measurement of collagen synthesis by human bone cells with a sirius red-based colorimetric microassay: effects of transforming growth factor beta 2 and ascorbic acid 2-phosphate. Histochem Cell Biol 112:271–276CrossRefPubMed
32.
Zurück zum Zitat Eleftheriou CS, Trakas NB, Tzartos SJ (1991) Cellular ageing related proteins secreted by human fibroblasts. Mutat Res 256:127–138CrossRefPubMed Eleftheriou CS, Trakas NB, Tzartos SJ (1991) Cellular ageing related proteins secreted by human fibroblasts. Mutat Res 256:127–138CrossRefPubMed
33.
Zurück zum Zitat Dunlevy JR, Couchman JR (1993) Controlled induction of focal adhesion disassembly and migration in primary fibroblasts. J Cell Sci 105:489–500PubMed Dunlevy JR, Couchman JR (1993) Controlled induction of focal adhesion disassembly and migration in primary fibroblasts. J Cell Sci 105:489–500PubMed
34.
Zurück zum Zitat Beurden HEV, Snoek PAM, Von den Hoff JW, Torensma R, Maltha JC, Kuijpers-Jagtman AM (2006) In vitro migration and adhesion of fibroblasts from different phases of palatal wound healing. Wound Rep Reg 14:66–71CrossRef Beurden HEV, Snoek PAM, Von den Hoff JW, Torensma R, Maltha JC, Kuijpers-Jagtman AM (2006) In vitro migration and adhesion of fibroblasts from different phases of palatal wound healing. Wound Rep Reg 14:66–71CrossRef
35.
Zurück zum Zitat Lallier TE, Miner QW Jr, Sonnier J, Spencer A (2007) A simple cell motility assay demonstrates differential motility of human periodontal ligament fibroblasts, gingival fibroblasts, and pre-osteoblasts. Cell Tissue Res 328:339–354CrossRefPubMed Lallier TE, Miner QW Jr, Sonnier J, Spencer A (2007) A simple cell motility assay demonstrates differential motility of human periodontal ligament fibroblasts, gingival fibroblasts, and pre-osteoblasts. Cell Tissue Res 328:339–354CrossRefPubMed
36.
37.
Zurück zum Zitat Desmet KD, Paz DA, Corry JJ, Eells JT, Wong-Riley MT, Henry MM (2006) Clinical and experimental applications of NIR-LED photobiomodulation. Photomed Laser Surg 24:121–128CrossRefPubMed Desmet KD, Paz DA, Corry JJ, Eells JT, Wong-Riley MT, Henry MM (2006) Clinical and experimental applications of NIR-LED photobiomodulation. Photomed Laser Surg 24:121–128CrossRefPubMed
38.
Zurück zum Zitat Kreisler M, Christoffers AB, Willershausen B, d’Hoedt B (2003) Effect of low-level GaAlAs laser irradiation on the proliferation rate of human periodontal ligament fibroblasts: an in vitro study. J Clin Periodontol 30:353–358CrossRefPubMed Kreisler M, Christoffers AB, Willershausen B, d’Hoedt B (2003) Effect of low-level GaAlAs laser irradiation on the proliferation rate of human periodontal ligament fibroblasts: an in vitro study. J Clin Periodontol 30:353–358CrossRefPubMed
39.
Zurück zum Zitat Posten W, Wrone DA, Dover JS, Arndt KA, Silapunt S, Alam M (2005) Low-level laser therapy for wound healing: mechanism and efficacy. Dermatol Surg 31:334–339CrossRefPubMed Posten W, Wrone DA, Dover JS, Arndt KA, Silapunt S, Alam M (2005) Low-level laser therapy for wound healing: mechanism and efficacy. Dermatol Surg 31:334–339CrossRefPubMed
40.
Zurück zum Zitat AlGhamdi KM, Kumar A, Moussa NA (2012) Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci 27:237–249CrossRefPubMed AlGhamdi KM, Kumar A, Moussa NA (2012) Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci 27:237–249CrossRefPubMed
41.
Zurück zum Zitat Pratsinis H, Kletsas D, Stathakos D (1997) Autocrine growth regulation in fetal and adult human fibroblasts. Biochem Biophys Res Commun 237:348–353CrossRefPubMed Pratsinis H, Kletsas D, Stathakos D (1997) Autocrine growth regulation in fetal and adult human fibroblasts. Biochem Biophys Res Commun 237:348–353CrossRefPubMed
42.
Zurück zum Zitat Yeo E, Jang I, Lim H, Ha K, Park SC (2002) Agonist-specific differential changes of cellular signal transduction pathways in senescent human diploid fibroblast. Exp Gerontol 37:871–883CrossRefPubMed Yeo E, Jang I, Lim H, Ha K, Park SC (2002) Agonist-specific differential changes of cellular signal transduction pathways in senescent human diploid fibroblast. Exp Gerontol 37:871–883CrossRefPubMed
43.
Zurück zum Zitat Shiraha H, Gupta K, Drabik K, Wells A (2000) Aging fibroblasts present reduced epidermal growth factor (EGF) responsiveness due to preferential loss of EGF receptors. J Biol Chem 275:19343–19351CrossRefPubMed Shiraha H, Gupta K, Drabik K, Wells A (2000) Aging fibroblasts present reduced epidermal growth factor (EGF) responsiveness due to preferential loss of EGF receptors. J Biol Chem 275:19343–19351CrossRefPubMed
44.
Zurück zum Zitat Bates DO, Jones ROP (2003) The roles of vascular endothelial growth factor in wound healing. Int J Low Extrem Wounds 2:107–120CrossRefPubMed Bates DO, Jones ROP (2003) The roles of vascular endothelial growth factor in wound healing. Int J Low Extrem Wounds 2:107–120CrossRefPubMed
Metadaten
Titel
Effects of low-level laser therapy and epidermal growth factor on the activities of gingival fibroblasts obtained from young or elderly individuals
verfasst von
Taisa Nogueira Pansani
Fernanda Gonçalves Basso
Ana Paula Silveira Turrioni
Diana Gabriela Soares
Josimeri Hebling
Carlos Alberto de Souza Costa
Publikationsdatum
27.09.2016
Verlag
Springer London
Erschienen in
Lasers in Medical Science / Ausgabe 1/2017
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-016-2081-x

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