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15.06.2019 | Original Article

Effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue: analysis by Raman spectroscopy, dual-energy X-ray absorptiometry, and mechanical resistance

verfasst von: Robson Chacon Castoldi, Guilherme Akio Tamura Ozaki, Thiago Alves Garcia, Ines Cristina Giometti, Tatiana Emy Koike, Regina Celi Trindade Camargo, João Domingos Augusto dos Santos Pereira, Carlos José Leopoldo Constantino, Mário Jefferson Quirino Louzada, José Carlos Silva Camargo Filho, William Dias Belangero

Erschienen in: Lasers in Medical Science | Ausgabe 2/2020

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Abstract

The aim of the present study was to verify the effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue by Raman spectroscopy (Raman), dual-energy X-ray absorptiometry (DXA), and mechanical resistance (F-max) analysis. A total of 40 male Wistar animals, 60 days old, were used. The animals were distributed into four groups: control (C), control with GH (GHC), muscular strength training (T), and muscular strength training with GH (GHT). Blood samples were collected for the quantification of creatine kinase (CK-MB) and the femurs were removed for analysis by Raman, DXA, and F-max. A more pronounced increase in the bone mineral components was verified in the T group, for all the variables obtained by the Raman (calcium, phosphate, amide, and collagen). In addition, for animals submitted to GH supplementation, there was a reduction in the variable bone mineral density (BMD) obtained by the DXA (p < 0.05). Finally, the animals that received GH supplementation presented a higher F-max, but without statistical significance (p > 0.05). It was concluded that animals that received GH supplementation demonstrated a decrease in BMD. In addition, T alone was able to promote increased calcium, phosphate, amide, and collagen compounds in bone tissue.

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Titel: Effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue: analysis by Raman spectroscopy, dual-energy X-ray absorptiometry, and mechanical resistance
verfasst von: Robson Chacon Castoldi
Guilherme Akio Tamura Ozaki
Thiago Alves Garcia
Ines Cristina Giometti
Tatiana Emy Koike
Regina Celi Trindade Camargo
João Domingos Augusto dos Santos Pereira
Carlos José Leopoldo Constantino
Mário Jefferson Quirino Louzada
José Carlos Silva Camargo Filho
William Dias Belangero
Publikationsdatum: 15.06.2019
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 2/2020
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-019-02821-5

Springer Medizin

Abstract

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