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09.08.2017 | Original Article

Effects of photobiomodulation therapy and topical non-steroidal anti-inflammatory drug on skeletal muscle injury induced by contusion in rats—part 2: biochemical aspects

verfasst von: Shaiane Silva Tomazoni, Lúcio Frigo, Tereza Cristina dos Reis Ferreira, Heliodora Leão Casalechi, Simone Teixeira, Patrícia de Almeida, Marcelo Nicolas Muscara, Rodrigo Labat Marcos, Andrey Jorge Serra, Paulo de Tarso Camillo de Carvalho, Ernesto Cesar Pinto Leal-Junior

Erschienen in: Lasers in Medical Science | Ausgabe 8/2017

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Abstract

Muscle injuries trigger an inflammatory process, releasing important biochemical markers for tissue regeneration. The use of non-steroidal anti-inflammatory drugs (NSAIDs) is the treatment of choice to promote pain relief due to muscle injury. NSAIDs exhibit several adverse effects and their efficacy is questionable. Photobiomodulation therapy (PBMT) has been demonstrated to effectively modulate inflammation induced from musculoskeletal disorders and may be used as an alternative to NSAIDs. Here, we assessed and compared the effects of different doses of PBMT and topical NSAIDs on biochemical parameters during an acute inflammatory process triggered by a controlled model of contusion-induced musculoskeletal injury in rats. Muscle injury was induced by trauma to the anterior tibial muscle of rats. After 1 h, rats were treated with PBMT (830 nm, continuous mode, 100 mW of power, 35.71 W/cm²; 1, 3, and 9 J; 10, 30, and 90 s) or diclofenac sodium (1 g). Our results demonstrated that PBMT, 1 J (35.7 J/cm²), 3 J (107.1 J/cm²), and 9 J (321.4 J/cm²) reduced the expression of tumor necrosis factor alpha (TNF-α) and cyclooxygenase-2 (COX-2) genes at all assessed times as compared to the injury and diclofenac groups (p < 0.05). The diclofenac group showed reduced levels of COX-2 only in relation to the injury group (p < 0.05). COX-2 protein expression remained unchanged with all therapies except with PBMT at a 3-J dose at 12 h (p < 0.05 compared to the injury group). In addition, PBMT (1, 3, and 9 J) effectively reduced levels of cytokines TNF-α, interleukin (IL)-1β, and IL-6 at all assessed times as compared to the injury and diclofenac groups (p < 0.05). Thus, PBMT at a 3-J dose was more effective than other doses of PBMT and topical NSAIDs in the modulation of the inflammatory process caused by muscle contusion injuries.

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Titel: Effects of photobiomodulation therapy and topical non-steroidal anti-inflammatory drug on skeletal muscle injury induced by contusion in rats—part 2: biochemical aspects
verfasst von: Shaiane Silva Tomazoni
Lúcio Frigo
Tereza Cristina dos Reis Ferreira
Heliodora Leão Casalechi
Simone Teixeira
Patrícia de Almeida
Marcelo Nicolas Muscara
Rodrigo Labat Marcos
Andrey Jorge Serra
Paulo de Tarso Camillo de Carvalho
Ernesto Cesar Pinto Leal-Junior
Publikationsdatum: 09.08.2017
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 8/2017
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-017-2299-2

Springer Medizin

Abstract

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