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12.12.2019 | Original Article

Effects of photobiomodulation therapy in the integration of skin graft in rats

verfasst von: Tatiane Neiva Santos Castro, Cintia Cristina Santi Martignago, Lívia Assis, Francisco Eugênio Deusdará de Alexandria, José Carlos Tatmatsu Rocha, Nivaldo Antonio Parizotto, Carla Roberta Tim

Erschienen in: Lasers in Medical Science | Ausgabe 4/2020

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Abstract

Skin graft is one of the most common techniques used in plastic surgery and repair. However, there are some complications that can lead to loss of the skin graft. Thus, several features have been studied with the aim of promoting the integration of skin grafts. Among these resources, the use of laser photobiomodulation (laser PBM) has been highlighted. The present study aimed to investigate the effects of laser PBM on the viability and integration of skin grafts in rats. Twenty male Wistar rats (± 250 g) were randomly assigned into two experimental groups with 10 animals each: control group, animals submitted to skin graft and simulation of laser PBM; laser PBM group, submitted to the skin graft and submitted to laser PBM at 660 nm, 40 mW, 60 s, 2.4 J. The animals were submitted to laser photobiomodulation immediately after the surgical procedure and each 24 h. Animal euthanasia occurred on the 7th day after surgery, 24 h after the last treatment session. The histopathological analysis revealed that the laser PBM showed better adhesion of the graft when compared to the control group. Likewise, the morphometric analysis of mast cells, blood vessels, and collagen showed a statistically significant increase in the animals irradiated with the laser PBM when compared to the control group. In addition, immunohistochemical analysis demonstrated that the laser PBM showed statistically higher immunoexpression of FGF when compared to the CG. However, IL-4 immunoexpression did not show statistical difference between the experimental groups. From the results obtained in the present study, it can be suggested that laser photobiomodulation was effective in promoting the integration and viability of total skin grafts in rats.

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Titel: Effects of photobiomodulation therapy in the integration of skin graft in rats
verfasst von: Tatiane Neiva Santos Castro
Cintia Cristina Santi Martignago
Lívia Assis
Francisco Eugênio Deusdará de Alexandria
José Carlos Tatmatsu Rocha
Nivaldo Antonio Parizotto
Carla Roberta Tim
Publikationsdatum: 12.12.2019
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 4/2020
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-019-02909-y

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Effects of photobiomodulation therapy in the integration of skin graft in rats

Abstract

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

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