Skip to main content
Hauptrubriken
CME Facharzt-Training Webinare Zeitschriften Bücher e.Medpedia Podcast
Service
Jobbörse Info & Hilfe
Fachgebiete
Anästhesiologie Allgemeinmedizin Arbeitsmedizin Augenheilkunde Chirurgie Dermatologie Gynäkologie und Geburtshilfe HNO Innere Medizin Kardiologie Neurologie Onkologie und Hämatologie Orthopädie und Unfallchirurgie Pädiatrie Pathologie Psychiatrie Radiologie Rechtsmedizin Urologie Zahnmedizin
Themen
Typ-2-Diabetes und Adipositas Klimawandel und Gesundheit Neues aus dem Markt Praxis und Beruf Seltene Erkrankungen GOÄ & EBM Panorama
Sammlungen
Kasuistiken Algorithmen & Infografiken Blickdiagnosen Arthropedia FlapFinder (für Dermatochirurgen) Videos Kongressberichterstattung Medizin für Apothekerinnen und Apotheker Für Ärztinnen und Ärzte in Weiterbildung Für Medizinstudierende Patientenratgeber
Erweiterte Suche
Anmelden
nach oben
Odontology
Erschienen in:

22.10.2016 | Original Article

Effects of photodynamic therapy, 2 % chlorhexidine, triantibiotic mixture, propolis and ozone on root canals experimentally infected with Enterococcus faecalis: an in vitro study

verfasst von: Fabio Camacho-Alonso, P. Salmerón-Lozano, Y. Martínez-Beneyto

Erschienen in: Odontology | Ausgabe 3/2017

Einloggen, um Zugang zu erhalten

Abstract

The aim of this study was to evaluate the antibacterial effect of photodynamic therapy (PDT), 2 % chlorhexidine (CHX). The teeth were contaminated with 0.1 mL Enterococcus faecalis (3 × 108 cell/mL) and randomized into eight treatment groups: Group 1 (2.5 % NaOCl); Group 2 (PDT); Group 3 (2 % CHX); Group 4 (TAM); Group 5 (propolis), Group 6 (ozone), Group 7 (positive control) and Group 8 (negative control). Following treatment, the canal content was sampled. The samples were cultured in triplicate on blood agar plates to determine the number of colony forming units (CFU)/mL. The teeth were analyzed by SEM to determine the percentage of area with contamination and debris. The group with the lowest CFU/mL count was the ozone group, which obtained similar values to the PDT group. SEM images showed that 2.5 % NaOCL group showed the lowest percentage of contaminated area. Applications of PDT, 2 % CHX, TAM, propolis and ozone showed antibacterial potential similar to 2.5 % NaOCL.
Literatur
1.
2.
Nair PN, Henry S, Cano V, Vera J. Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after “one visit” endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005;99:231–52.CrossRefPubMed
3.
Pagonis TC, Chen J, Fontana CR, et al. Nanoparticle-based endodontic antimicrobial photodynamic therapy. J Endod. 2010;36:322–8.CrossRefPubMed
4.
Cheng X, Guan S, Lu H, et al. Evaluation of the bacterial effect of Nd:YAG, Er:YAG, Er, Cr:YSGG laser radiation, and antimicrobial photodynamic therapy (aPDT) in experimentally infected root canals. Lasers Sur Med. 2012;44:824–31.CrossRef
5.
Love RM. Enterococcus faecalis-a mechanism for its role in endodontic failure. Int Endod J. 2001;34:399–405.CrossRefPubMed
6.
Estrela C, Estrela CR, Decurcio DA, Hollanda AC, Silva JA. Antimicrobial effecacy of ozonated water, gaseous ozone, sodium hypochlorite and chlorhexidine in infected human root canals. Int J Endod J. 2007;40:85–93.CrossRef
7.
Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod. 2006;32:93–8.CrossRefPubMed
8.
Estrela C, Sousa-Nieto MD, Alves DR, Alencar H, Santos TO, Pécora JD. A preliminary study of the antibacterial potential of cetylpyridinium chloride in root canals infected by E. faecalis. Braz Den J. 2012;23:645–53.CrossRef
9.
Kobayashi Y, Hayashi M, Yoshino F, et al. Bactericidal effect of hydroxyl radicals generated from a low concentration hydrogen peroxide with ultrasound in endodontic treatment. J Clin Biochem Nutr. 2014;54:161–5.CrossRefPubMedPubMedCentral
10.
11.
Spangberg L, Engstrom B, Langeland K. Biologic effects of dental materials 3. Toxicity and antimicrobial effect of endodontic antiseptics in vitro. Oral Surg Oral Med Oral Pathol. 1973;36:856–71.CrossRefPubMed
12.
Hoshino E, Takushige T. LSTR 3MIX-MP method-better and efficient clinical procedures of lesion sterilization and tissue repair (LSTR) therapy. Dent Rev. 1998;666:57–106.
13.
Takushige T, Cruz E, Moral A, Hoshino E. Endodontic treatment of primary teeth using a combination of antibacterial drugs. Int Endod J. 2004;37:132–8.CrossRefPubMed
14.
Bankova V. Chemical diversity of propolis and the problem of standardization. J Ethnopharmacol. 2005;100:114–7.CrossRefPubMed
15.
Kayaoglu G, Ömürlü H, Akca G, et al. Antibacterial activity of propolis versus conventional endodontic desinfectants against Enterococcus faecalis in infected dentinal tubules. J Endod. 2011;37:376–81.CrossRefPubMed
16.
Navarro-Escobar E, González-Rodríguez MP, Ferrer-Luque CM. Cytotoxic effects of two acid solutions and 2.5% sodium hypochlorite used in endodontic therapy. Med Oral Patol Oral Cir Bucal. 2010;15:90–4.
17.
Peters LB, Wesselink PR, Buijs JF, van Winkelhoff AJ. Viable bacteria in root dentinal tubules of teeth with apical periodontitis. J Endod. 2001;27:76–81.CrossRefPubMed
18.
Pinheiro SL, Schenka AA, Neto AA, de Souza CP, Rodriguez HM, Ribeiro MC. Photodynamic therapy in endodontic treatment of deciduous teeth. Lasers Med Sci. 2009;24:521–6.CrossRefPubMed
19.
Maish T, Bosl C, Szeimies RM, Lehn N, Abels C. Photodynamic effects of novel XF porphyrin derivates on prokaryotic and eukaryotic cells. Antimicrob Agents Chemother. 2005;49:1542–52.CrossRef
20.
Noites R, Pina-Vaz C, Rocha R, Carvalho MF, Gonçalves A, Pina-Vaz I. Synergistic antimicrobial action of chlorhexidine and ozone in endodontic treatment. Biomed Res Int. 2014;2014:592423.CrossRefPubMedPubMedCentral
21.
Sawadaishi K, Miura K, Ohtsuka E, Ueda T, Ishizaki K, Shinriki N. Ozonolysis of supercoloid pBR322 DNA resulting in strand scission to open circular DNA. Nucleic Acids Res. 1985;13:7183–94.CrossRefPubMedPubMedCentral
22.
Reddy S, Reddy N, Dinapadu S, Reddy M, Pasari S. Role of ozone therapy in minimal intervention dentistry and endodontics – a review. J Int Oral Health. 2013;5:102–8.PubMedPubMedCentral
23.
24.
Haapasalo M, Orstavik D. In vitro infection and disinfection of dentinal tubules. J Dent Res. 1987;66:1375–9.CrossRefPubMed
25.
Seal GJ, Ng YL, Spratt D, Bhatti M, Gulabivala K. An in vitro comparison of the bacterial efficacy of lethal photosensitization or sodium hypochloride irrigation on Streptococcus intermedius biofilms in root canals. Int Endod J. 2002;35:268–74.CrossRefPubMed
26.
Schäfer E, Böosmann K. Antimicrobial efficacy of chlorhexidine and two calcium hydroxide formulation against Enterococcus faecalis. J Endod. 2005;31:53–6.CrossRefPubMed
27.
Madhubala MM, Srinivasan N, Ahamed S. Comparative evaluation of propolis and triantibiotic mixture as an intracanal medicament against Enterococcus faecalis. J Endod. 2011;37:1287–9.CrossRefPubMed
28.
Shingare P, Chaugule V. Comparative evaluation of antimicrobial activity of miswak, propolis, sodium hypochlorite and saline as root canal irrigants by microbial culturing and quantification in chronically exposed primary teeth. Germs. 2011;24:12–21.
29.
Souza LC, Brito PR, de Oliveira JC, et al. Photodynamic therapy with two different photosensitizers as a supplement to instrumentation/irrigation procedures in promoting intracanal reduction of Enterococcus faecalis. J Endod. 2010;36:292–6.CrossRefPubMed
30.
Fimple JL, Fontana CR, Foschi F, Ruggiero K, Song X, Pagonis TC. Photodynamic treatment of endodontic polymicrobial infection in vitro. J Endod. 2008;34:728–34.CrossRefPubMedPubMedCentral
31.
Heling I, Rotstein I, Dinur T, Szwec-Levine Y, Steinberg D. Bacterial and cytotoxic effects of sodium hypochlorite and sodium dichloroisocyanurate solutions in vitro. J Endod. 2001;27:278–80.CrossRefPubMed
32.
Baumgartner JC, Cuenin PR. Efficacy of several concentrations of sodium hypoclorite for root canal irrigation. J Endod. 1992;18:605–12.CrossRefPubMed
33.
Gernhardt CR, Eppendorf K, Kozlowski A, Brandt M. Toxicity of concretated sodium hypochlorite used as an endodontic irrigant. Int Endod J. 2004;37:272–80.CrossRefPubMed
34.
Zou L, Shen Y, Li W, Haapasalo M. Penetration of sodium hypochlorite into dentin. J Endod. 2010;36:793–6.CrossRefPubMed
35.
Evanov C, Liewehr FR, Buxlon TB, Joyce AP. Antibacterial efficacy of calcium hydroxide and chlorhexidine irrigants at 37 °C and 46 °C. J Endod. 2004;30:653–7.CrossRefPubMed
36.
Ferguson JW, Hatton JF, Gillespie MJ. Effectiveness of intracanal irrigants and medications against the yeast Candida albicans. J Endod. 2002;28:68–71.CrossRefPubMed
37.
Sassone LM, Fidel R, Fidel S, Vieira M, Hirata R Jr. The influence of organic load on the antimicrobial activity of different concentrations of NaOCl and chlorhexidine in vitro. Int Endod J. 2003;36:848–52.CrossRefPubMed
38.
Naenni N, Thoma K, Zhender M. Soft tissue dissolution capacity of currently used and potential endodontic irrigants. J Endod. 2004;30:785–7.CrossRefPubMed
39.
Flach N, Böttcher DE, Parolo CC, et al. Confocal microscopy evaluation of the effect of irrigants on Enterococcus faecalis biofilm: an in vitro study. Scanning. 2016;38:57–62.CrossRefPubMed
40.
Chhabra RS, Huff JE, Haseman JK, Elwell MR, Peters AC. Carcinogenicity of p-chloroaniline in rats and mice. Food Chem Toxicol. 1991;29:119–24.CrossRefPubMed
41.
Adl A, Shojaee NS, Motamedifar M. A comparison between the antimicrobial effects of triple antibiotic paste and calcium hydroxide against Enterococcus faecalis. Iran Endod J. 2012;7:149–55.PubMedPubMedCentral
42.
Hoshino E, Kurihara-ando N, Sato I, et al. In-vitro antibacterial susceptibility of bacteria taken from infected root dentine to a mixture of ciprofloxacin, metronidazole and minocycline. Int Endod J. 1996;29:125–30.CrossRefPubMed
43.
Huang GT. A paradigm shift in endodontic management of immature teeth: conservation of stem cells for regeneration. J Dent. 2008;36:379–86.CrossRefPubMed
44.
Rios A, He J, Glickman GN, Spears R, Schneiderman ED, Honeyman AL. Evaluation of photodynamic therapy using a light-emitting diode lamp against Enterococcus faecalis in extracted human teeth. J Endod. 2011;37:856–9.CrossRefPubMed
45.
Nagayoshi M, Fukuizumi T, Kitamura C, Yano J, Terashita M, Nishihara T. Efficacy of ozone on survival and permeability of oral microorganisms. Oral Microbiol Immunol. 2004;19:240–6.CrossRefPubMed
46.
Kustarci A, Sumer Z, Altunbas D, Kosum S. Bactericidal effect of KTP laser irradiation against Enterococcus faecalis compared with gaseous ozone: an ex vivo study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;107:e73–9.CrossRefPubMed
47.
Saha S, Nair R, Asrani H. Comparative evaluation of propolis, metronidazole with chlorhexidine, calcium hydroxide and curcuma longa extract as intracanal medicament against E. faecalis—an invitro study. J Clin Diagn Res. 2015;9:ZC19–21.PubMedPubMedCentral
48.
Mirzoeva OK, Grishanin RN, Calder PC. Antimicrobial action of propolis and some of its components: the effects on growth, membrane potential and motility of bacteria. Microbiol Res. 1997;152:239–46.CrossRefPubMed
49.
Paqué F, Boessler C, Zehnder M. Accumulated hard debris level in mesial roots of mandibular molars after sequential irrigation steps. Int Endod J. 2011;44:148–53.CrossRefPubMed
Metadaten
Titel
Effects of photodynamic therapy, 2 % chlorhexidine, triantibiotic mixture, propolis and ozone on root canals experimentally infected with Enterococcus faecalis: an in vitro study
verfasst von
Fabio Camacho-Alonso
P. Salmerón-Lozano
Y. Martínez-Beneyto
Publikationsdatum
22.10.2016
Verlag
Springer Japan
Erschienen in
Odontology / Ausgabe 3/2017
Print ISSN: 1618-1247
Elektronische ISSN: 1618-1255
DOI
https://doi.org/10.1007/s10266-016-0271-4

Weitere Artikel der Ausgabe 3/2017

Functional effects of cold stimulation on taste perception in humans

  • Original Article

Correlation between resonance frequency, insertion torque and bone-implant contact in self-cutting threaded implants

  • Original Article

Soft tissue changes after a mandibular osteotomy for symmetric skeletal class III malocclusion

  • Original Article

Dental arch spatial changes after premature loss of first primary molars: a systematic review of controlled studies

  • Original Article

Caries diagnosis using light fluorescence devices in comparison with traditional visual and tactile evaluation: a prospective study in 152 patients

  • Original Article

Marginal integrity of low-shrinking versus methacrylate-based composite: effect of different one-step self-etch adhesives

  • Original Article

Neu im Fachgebiet Zahnmedizin

Das alles ändert sich für Arztpraxen in 2025

  • 31.12.2024
  • EBM
  • Nachrichten

Ab Januar greifen jede Menge Neuerungen – ob im EBM, Arbeits- und Steuerrecht oder bei der digitalen Vernetzung. Darunter einige Verbesserungen, aber teils auch Mehraufwand. „Same procedure as every year?“ – Ein Überblick.

Wenn Praxen Termine absagen müssen: So geht’s ohne Ärger und Stress

Bundesweit sind viele Praxen von Krankheitsfällen in den eigenen Reihen betroffen. Die Praxis-EDV und strukturierte Abläufe können notwendige Terminabsagen erleichtern.

Wie gefährlich sind suspekte Veränderungen in der Mundhöhle?

Wie hoch ist das Risiko, dass sich aus einer bereits verdächtigen Veränderung der Mundschleimhaut ein Karzinom entwickelt? Ein US-Team hat dazu das Patientenregister der Universität San Francisco ausgewertet.

Das deutsche Gesundheitssystem kränkelt und hat Fieber

OECD und Europäische Kommission haben zusammen einen Bericht zum Zustand der europäischen Gesundheitssysteme herausgegeben. Diagnose für Deutschland: Wäre das Land ein menschliches Wesen, würde man es wohl krankschreiben.

Update Zahnmedizin

Bestellen Sie unseren kostenlosen Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen
Bildnachweise