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07.06.2018 | Original Paper

Effects of press-fit biphasic (collagen and HA/βTCP) scaffold with cell-based therapy on cartilage and subchondral bone repair knee defect in rabbits

verfasst von: Jacques Hernigou, Pascale Vertongen, Esfandiar Chahidi, Theofylaktos Kyriakidis, Jean-Paul Dehoux, Magalie Crutzen, Sébastien Boutry, Lionel Larbanoix, Sarah Houben, Nathalie Gaspard, Dimitrios Koulalis, Joanne Rasschaert

Erschienen in: International Orthopaedics | Ausgabe 7/2018

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Abstract

Introduction

Human spontaneous osteonecrosis of the knee (SPONK) is still challenging as the current treatments do not allow the production of hyaline cartilage tissue. The aim of the present study was to explore the therapeutic potential of cartilage regeneration using a new biphasic scaffold (type I collagen/hydroxyapatite) previously loaded or not with concentrated bone marrow cells.

Material and methods

Female rabbits were operated of one knee to create articular lesions of the trochlea (three holes of 4 × 4mm). The holes were left empty in the control group or were filled with the scaffold alone or the scaffold previously loaded with concentrated bone marrow cells. After two months, rabbits were sacrificed and the structure of the newly formed tissues were evaluated by macroscopic, MRI, and immunohistochemistry analyses.

Results

Macroscopic and MRI evaluation of the knees did not show differences between the three groups (p > 0.05). However, histological analysis demonstrated that a higher O’Driscoll score was obtained in the two groups treated with the scaffold, as compared to the control group (p < 0.05). The number of cells in treated area was higher in scaffold groups compared to the control group (p < 0.05). There was no difference for intensity of collagen type II between the groups (p > 0.05) but subchondral bone repair was significantly thicker in scaffold-treated groups than in the control group (1 mm for the control group vs 2.1 and 2.6 mm for scaffold groups). Furthermore, we observed that scaffolds previously loaded with concentrated bone marrow were more reabsorbed (p < 0.05).

Conclusion

The use of a biphasic scaffold previously loaded with concentrated bone marrow significantly improves cartilage lesion healing.

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Titel: Effects of press-fit biphasic (collagen and HA/βTCP) scaffold with cell-based therapy on cartilage and subchondral bone repair knee defect in rabbits
verfasst von: Jacques Hernigou
Pascale Vertongen
Esfandiar Chahidi
Theofylaktos Kyriakidis
Jean-Paul Dehoux
Magalie Crutzen
Sébastien Boutry
Lionel Larbanoix
Sarah Houben
Nathalie Gaspard
Dimitrios Koulalis
Joanne Rasschaert
Publikationsdatum: 07.06.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: International Orthopaedics / Ausgabe 7/2018
Print ISSN: 0341-2695
Elektronische ISSN: 1432-5195
DOI: https://doi.org/10.1007/s00264-018-3999-3

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Springer Medizin

Effects of press-fit biphasic (collagen and HA/βTCP) scaffold with cell-based therapy on cartilage and subchondral bone repair knee defect in rabbits

Abstract

Introduction

Material and methods

Results

Conclusion

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Springer Medizin

Abstract

Introduction

Material and methods

Results

Conclusion

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