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01.12.2015 | Research article | Ausgabe 1/2015 Open Access

BMC Musculoskeletal Disorders 1/2015

Effects of prostaglandin E1 on callus formation in rabbits

BMC Musculoskeletal Disorders > Ausgabe 1/2015
Pawel V. Lipinsky, Ivan V. Sirotin, Alexandr V. Skoroglyadov, Alexey V. Ivkov, Alexandr P. Oettinger, Evgeny E. Krynetskiy, Alexandr B. But-Gusaim, Andreas J. Roth
Wichtige Hinweise
Pawel V. Lipinsky and Ivan V. Sirotin contributed equally to this work.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

PVL and IVS performed critical parts of all surgical procedures in this study, contributed gross specimen measurements and evaluation of data and statistical analysis. They drafted, revised, finalized, and submitted the manuscript. AVS and ABG participated in the experimental and organizational design of the study, supervised the evaluation and interpretation of the experimental results, reviewed the manuscript, presented the case to the IRB board for approval, and secured financial support and laboratory space for the experiments. AVI and APO participated in the preparation of the animals, fixation of the specimens, and reviewed the manuscript. EEK actively participated in the experimental and organizational design of the study, gave valuable advice during the evaluation and interpretation of the experimental results, and reviewed the manuscript. AR reviewed all experimental data, gave valuable advice during evaluation and interpretation of the experimental results and subsequent conclusions, contributed histological evaluation of the samples, and reviewed the manuscript. All authors have read and approved the final version of the manuscript.



Recent research has focused on identifying chemical modulators of osteogenesis. We present initial findings on the osteoinductive properties of prostaglandin Е1 (Vasaprostan), using a rabbit model.


Data were collected on callus formation in 14 male rabbits. These were divided into two groups (control and treatment) with 7 animals in each group. In all animals, the right tibia was fractured using a standardized protocol and stabilized by an intramedullary nail. Treatment group received a 5 μg/kg subcutaneous injection of PGE1/day during 10 postoperative days. Visual and radiological evaluation of callus formation was prospectively collected. After 30 days, all animals were killed and the tibia specimens were examined histologically.


In all the treatment group animals, fractures were consolidated radiologically by day 30. No treatment group animals and two control group animals were excluded form the experiment. In the control group, 4 animals demonstrated slower callus formation than the main group. Two control group animals were excluded from the experiment on the 20th day due to wound infection; one developed a nonunion.
The mean coefficient of bone callus thickening in the main group was 2.08 (±0, 16) and 1.77 (±0.05) (p < 0.05) in the control group. Calculation of mean quantity of neogenic vessels in 10 random visual fields of the bone callus revealed 78 (±9.82) in the main group and 40 (±4.68) in the control group (p < 0.05).


Our study demonstrates an increased rate and amount of bone callus formation in the group treated with prostaglandin E1 compared to the control group. Prospective radiological analysis was corroborated by histologic evaluation.
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