Background
Pulmonary arterial hypertension (PAH) is a frequent and severe complication in patients with connective tissue diseases (CTD), a group of disorders which adversely affects the cardiac and the respiratory system. It is also known to have non-respiratory complications, such as renal disorders [
1]. CTD is characterised by vascular injury, autoimmunity, tissue inflammation and organ dysfunction including systemic sclerosis (SSc), systemic lupus erythematosus (SLE) and mixed CTD [
2]. PAH affects approximately 3 to 13% of CTD patients (CTD-PAH), and it is one of the leading causes of death [
3‐
5]. A recent registry in China has reported that out of all patients diagnosed with PAH, 43% had CTD-PAH [
6]. High doses of steroids and immune suppressants have demonstrated beneficial effect in the treatment of CTD-PAH [
7]. The use of vasodilators has been recommended in WHO functional class II to class IV patients [
8]. Since immunosuppressive therapy does not provide long-term benefits, it is recommended to use vasodilators in combination with immunosuppressants for the treatment of CTD-PAH [
1,
9,
10].
Ambrisentan, a vasodilator, which is an endothelin-A (ET
A) receptor-selective antagonist. It is approved for the treatment of patients with PAH (World Health Organisation [WHO] functional class [FC] II or III symptoms) at oral doses of 5 and 10 mg once daily. The efficacy of ambrisentan, both alone and in combination, has been evaluated in previous studies in patients with CTD-PAH and the results have demonstrated an improvement in exercise capacity [
11,
12] and freedom from clinical worsening in 80% of the patients at 1 year [
11]. These studies included higher proportion of Caucasians and patients of Asian ethnicity were very limited.
The available data suggest that patients with CTD-PAH may have an attenuated response to short-term treatment than other forms of PAH [
13], though they have a similar response to long-term treatment [
14]. This could be explained by the heterogeneity in the treatment response in patients with PAH and wide variety of significant comorbidities such as rheumatoid arthritis, dermatomyositis, polymyositis, interstitial lung disease, limited mobility and deconditioning commonly seen in patients with CTD-PAH [
15,
16]. In addition to the observed heterogeneity in treatment response, there is insufficient data in the existing literature for CTD-PAH subgroup that necessitate the understanding of specific therapeutic needs in managing individuals with different ethnicities. In the recent study conducted in Chinese patients with PAH, it was shown that ambrisentan improved exercise capacity, measured by 6-min walk test (6MWT) in the 24-week treatment period [
17]. This study included about 53% patients with CTD at baseline, thus providing an opportunity to evaluate whether ambrisentan is efficacious in patients with CTD-PAH to the same extent as in the overall PAH population. The present post-hoc subgroup analysis was performed to evaluate the efficacy of ambrisentan in improving exercise capacity in Chinese patients with CTD-PAH. Additionally, we provided data on the patients with idiopathic PAH/heritable PAH (IPAH/HPAH) to explore the difference in response to treatment based on underlying disease.
Methods
Study design
An open-label, phase IIIb, single-arm study was conducted in China in patients with PAH between 21 December 2012 and 15 August 2014 (
NCT01808313). The detailed study design and results of the study have been reported elsewhere [
17]. This post-hoc analysis was conducted using a subgroup of patients with CTD-PAH and IPAH/HPAH. Briefly, eligible patients received oral 5 mg ambrisentan once daily for 12 weeks in the primary evaluation period, followed by a 12-week dose adjustment period, during which the dose of ambrisentan was titrated to 10 mg depending on patients’ dose tolerance [
17].
All patients provided written informed consent before any study-specific procedure was performed. The study was conducted in accordance with International Conference on Harmonisation guidelines for Good Clinical Practice and the ethical principles laid down in the Declaration of Helsinki (2008). The study protocol was approved by independent ethics committee or institutional review board at each centre. The affiliations of all the ethics committees (IECs) that approved the study are available in the additional file. Anonymised individual participant data and study documents can be requested for further research from
www.clinicalstudydatarequest.com.
Patients
Patients aged 18–75 years who had a diagnosis of symptomatic or severe PAH (WHO FC II or III), as defined in Group 1 of the current treatment guidelines [
18], were enrolled in the study. Patients were included if they performed the 6MWT with a minimum distance of 150 m and a maximum distance of 450 m, had a right heart catheterisation performed within 6 months of screening with mean pulmonary artery pressure ≥ 25 mmHg; pulmonary vascular resistance ≥240
dyne.sec.cm− 5; and pulmonary capillary wedge pressure or left ventricular end diastolic pressure ≤ 15 mmHg. Patients with serum transaminase values > 2 times upper limit of normal (ULN), serum bilirubin > 1.5 times ULN, or haemoglobin concentration < 10 g/dL at baseline were excluded from the study. CTD patients were in an inactive state after the treatment of steroid with or without immunosuppresants. The detailed inclusion and exclusion criteria of patients have been described previously [
17].
Study assessments
This subgroup analysis assessed all primary and secondary endpoints from the primary study except for echocardiography assessments. The primary efficacy endpoint was change from baseline to week 12 in the exercise capacity, as measured by 6MWT. Other efficacy endpoints included change in 6MWT from baseline to week 24 and change in N-terminal pro B type natriuretic peptide (NT-proBNP) plasma levels, WHO FC and Borg Dyspnoea Index (BDI) scores from baseline to weeks 12 and 24. Changes in 6MWT, WHO FC and BDI scores were assessed at every visit, scheduled at 4-week intervals and changes in NT-pro BNP levels were assessed at weeks 12 and 24. Additionally, the effect of ambrisentan on heart rate recovery (defined as difference in heart rate at the end of 6MWT and at 1/2/3 min after completion of the 6MWT) at 1 min (HRR1min), 2 min (HRR2min) and 3 min (HRR3min) post-exercise was assessed.
Time to clinical worsening of PAH (defined as time from baseline to first occurrence of death, lung transplantation, hospitalisation for PAH treatment, atrial septostomy or discontinuation of ambrisentan due to change to other PAH treatment) was assessed.
Safety and tolerability assessments included monitoring and recording of adverse events (AEs) and serious AEs (SAEs), laboratory assessments, liver function tests, 12-lead electrocardiogram (ECG) and vital sign measurements.
Statistical analysis
Efficacy analyses were performed on the intent-to-treat (ITT) population, which consisted of all patients with CTD-PAH and IPAH/HPAH who received at least one dose of ambrisentan and had baseline and at least one post-baseline efficacy assessment.
The change from baseline in 6MWT was assessed using a paired t-test. Changes in plasma NT-proBNP levels and BDI were assessed using a Wilcoxon Signed-Rank test. WHO FC outcomes were summarised descriptively in both CTD-PAH and IPAH/HPAH subgroups and the comparison between the CTD-PAH and IPAH/HPAH subgroups was performed using Chi-square test. The change in 6MWT was compared between CTD-PAH and IPAH/HPAH subgroups using a 2-group t-test. The last observation carried forward (LOCF) method was used to impute missing data for 6MWT, WHO FC and BDI assessments.
Time to clinical worsening was reported using Kaplan-Meier estimates along with corresponding 95% confidence intervals. Overall safety data were summarised descriptively. The safety population included all patients with CTD-PAH and IPAH/HPAH who received at least one dose of ambrisentan. Statistical analyses were conducted using SAS® Version 9.4.
Discussion
The results of this subgroup analysis showed that ambrisentan treatment significantly improved exercise capacity, as measured by the 6MWT, in Chinese patients with CTD-PAH as well as IPAH/HPAH. It was noteworthy that almost all the patients (97%) were women. Differential signalling, altered levels and altered metabolism of oestrogen have been reported to be the underlying factors responsible for the frequent occurrence of PAH in women than in men [
19]. Almost all the patients in the CTD-PAH subgroup showed no clinical worsening up to 24 weeks of treatment with ambrisentan.
Following 12 weeks of treatment, the exercise capacity was significantly improved for patients in both CTD-PAH and IPAH/HPAH subgroups. The increase was noted further up to 24 weeks of treatment. The improvement seen in the CTD-PAH subgroup was more pronounced than that observed in the IPAH/HPAH subgroup; however, the between-group difference was not statistically significant. The improvement in exercise capacity in patients with CTD-PAH was also greater than that observed in the overall Chinese PAH population (53.6 m at week 12 and 64.4 m at week 24 from a baseline of 377.1 m) [
17]. Additionally, the improvement in exercise capacity observed in this study in Chinese patients with CTD-PAH was substantially higher than that observed in a previous study in Caucasian patients with CTD-PAH [
12,
20]. Although in this study, the magnitude of increase in 6MWT was greater in Chinese patients compared with Caucasians in other studies; this could be due to difference in disease characteristics of PAH associated with CTD between Chinese and Caucasian populations. The increased prevalence of SLE as underlying disease in Chinese patients compared with SSc in Caucasians may have resulted in better improvement in 6MWT in this subgroup analysis [
12,
21].
The increased exercise capacity in 6MWT after treatment with ambrisentan was further supported by the significant improvement in BDI scores for patients with CTD-PAH but not for patients with IPAH/HPAH. In patients with CTD-PAH, the improvement seen in BDI scores was higher than the corresponding improvement seen in the overall population in the study (− 0.6 vs. -0.2 at week 12 and − 0.4 vs. -0.2 at week 24) indicating the pronounced effect of ambrisentan in CTD-PAH population [
17]. These results are in agreement with previous studies, where improvement in BDI scores were observed at 12 and 24 weeks with both 5 mg and 10 mg doses [
13,
22].
Clinical improvements were also observed in other efficacy parameters in this analysis. The NT-proBNP levels were significantly reduced in both CTD-PAH as well as IPAH/HPAH subgroups at 12 weeks of treatment, but the effect was more pronounced in the CTD-PAH subgroup. CTD-PAH and IPAH/HPAH subgroups did not show further improvements in plasma BNP levels, following 24 weeks of treatment. The decrease in NT-proBNP levels at 12 weeks of treatment with ambrisentan in patients with CTD-PAH was slightly higher to that observed in the overall PAH population in the study [
17]. In contrast, there was 30 and 45% reduction in BNP levels at 5 mg and 10 mg doses of ambrisentan, respectively, in the ARIES-1 study [
13].
Considering the progressive nature of disease, it is important to note the effect of ambrisentan on WHO FC. More than 40% of CTD-PAH patients and approximately 30% of IPAH/HPAH patients showed improvement in WHO FC in the present analysis. Among the overall PAH population in the study, 33.1 and 38.3% of the patients showed improvement in WHO FC at 12 and 24 weeks, respectively [
17]. In the IPAH/HPAH subgroup, majority of the patients had not shown improvement in WHO FC, indicating less pronounced effect of ambrisentan in this subgroup. Previously, ambrisentan treatment has shown improvement in WHO FC in dose groups ranging from 2.5–10 mg in 12 weeks as well as 2 years [
13,
22].
The heart rate recovery at 1 and 2 min post-exercise was faster following ambrisentan treatment at week 24. This finding was of significance as heart rate recovery post-exercise is known to be a strong predictor of clinical worsening and survival in patients with PAH [
23,
24].
The majority of the AEs observed with ambrisentan were mild to moderate in intensity. The safety findings observed in the present analysis were similar to that in the earlier studies conducted in Western and overall Chinese PAH population [
13,
17,
22,
24].
The results of the current subgroup analysis showed that the efficacy of ambrisentan is at least of a similar magnitude to, and possibly greater in, CTD/PAH than IPAH/HPAH. However, due to limited sample size of the subgroup and short-term treatment duration, definite conclusions cannot be derived. Nonetheless, results of the current analysis provide substantial evidence regarding the efficacy of ambrisentan in CTD-PAH population and warrant further research in this direction.
Acknowledgements
The authors acknowledge the support of investigators, staff and study volunteers for participation in the study. Medical writing assistance was provided by Sanchika Agarwal and Ruchi Gupta (Tata Consultancy Services, India) and funded by GlaxoSmithKline.
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