Elotuzumab for the treatment of extramedullary myeloma: a retrospective analysis of clinical efficacy and SLAMF7 expression patterns
verfasst von:
Sophia Danhof, Leo Rasche, Anja Mottok, Tabea Steinmüller, Xiang Zhou, Martin Schreder, Teresa Kilian, Susanne Strifler, Andreas Rosenwald, Michael Hudecek, Hermann Einsele, Elena Gerhard-Hartmann
Extramedullary disease (EMD) represents a high-risk state of multiple myeloma (MM) associated with poor prognosis. While most anti-myeloma therapeutics demonstrate limited efficacy in this setting, some studies exploring the utility of chimeric antigen receptor (CAR)-modified T cells reported promising results. We have recently designed SLAMF7-directed CAR T cells for the treatment of MM. SLAMF7 is a transmembrane receptor expressed on myeloma cells that plays a role in myeloma cell homing to the bone marrow. Currently, the only approved anti-SLAMF7 therapeutic is the monoclonal antibody elotuzumab, but its efficacy in EMD has not been investigated thoroughly. Thus, we retrospectively analyzed the efficacy of elotuzumab-based combination therapy in a cohort of 15 patients with EMD. Moreover, since the presence of the target antigen is an indispensable prerequisite for effective targeted therapy, we investigated the SLAMF7 expression on extramedullary located tumor cells before and after treatment. We observed limited efficacy of elotuzumab-based combination therapies, with an overall response rate of 40% and a progression-free and overall survival of 3.8 and 12.9 months, respectively. Before treatment initiation, all available EMD tissue specimens (n = 3) demonstrated a strong and consistent SLAMF7 surface expression by immunohistochemistry. Furthermore, to investigate a potential antigen reduction under therapeutic selection pressure, we analyzed samples of de novo EMD (n = 3) outgrown during elotuzumab treatment. Again, immunohistochemistry documented strong and consistent SLAMF7 expression in all samples. In aggregate, our data point towards a retained expression of SLAMF7 in EMD and encourage the development of more potent SLAMF7-directed immunotherapies, such as CAR T cells.
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Introduction
Multiple myeloma (MM) is a malignant plasma cell dyscrasia that accounts for approximately 10% of all hematological malignancies. Albeit primarily a disease of the bone marrow, extramedullary myeloma manifestations occur in up to 37% of patients [28], and incidence increases over the course of the disease [3]. It has been postulated that myeloma cells must undergo relevant alterations of the adhesion, migration, and/or chemokine receptor profile [34] in order to lose their strong dependence on the bone marrow microenvironment and to obtain capacities required for extramedullary spread. In patients with extramedullary disease (EMD), high-risk cytogenetics are overrepresented [1, 4, 9, 31]. Furthermore, an association of altered expression of specific non-coding RNA molecules and EMD has been reported [2, 18]. However, the exact molecular mechanisms underlying the development of EMD remain largely unclear. Clinical studies consistently demonstrate an inferior prognosis for patients with EMD, with overall survival periods of less than 12 months in the relapsed/refractory setting in some cohorts [16, 40, 42]. In non-randomized treatment series, even regimens containing highly potent novel agents such as carfilzomib [42], pomalidomide [20], and daratumumab [39] achieve response rates of less than 30% in this context. Despite the urgent medical need, randomized clinical studies explicitly investigating the treatment of EMD are yet to be performed.
Based on the promising results of the ELOQUENT-2 trial, elotuzumab was the first monoclonal antibody that gained FDA/EMA approval [11] for patients with relapsed/refractory MM (RRMM) in combination with dexamethasone and lenalidomide. Subsequently, the ELOQUENT-3 trial [10] reported encouraging data for elotuzumab in combination with pomalidomide and dexamethasone in heavily pretreated patients with an overall response rate (ORR) of 53% and a median progression-free survival (PFS) of 10.3 months, leading to the additional approval of this combination therapy. Regrettably, although patients with EMD were included in the approval studies, detailed treatment response and outcome data for this subgroup have so far not been reported. Elotuzumab targets the signaling lymphocytic activation molecule (SLAM) family member 7 (SLAMF7, aliases: CD319, CS1, CRACC), an immunomodulatory transmembrane receptor that is highly and uniformly expressed on malignant plasma cells [19], natural killer cells, and other immune cells [6, 15]. SLAMF7 is a homotypic receptor that plays a role for myeloma cell homing to the bone marrow niche [38]. However, the downstream signaling network induced upon SLAMF7 activation in myeloma cells-and thus the functional relevance of SLAMF7 expression for the maintenance of the disease-remains unclear to date [17].
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As in other tumor entities, the concept of chimeric antigen receptor (CAR)-modified T cells opens up novel treatment options for patients suffering from MM. CAR-modified T cells targeting the B-cell maturation antigen (BCMA, CD269) have recently demonstrated promising efficacy in high-risk RRMM [33], including deep responses in patients with substantial extraosseous disease [5, 7, 29, 35]. However, the emergence of antigen loss variants during therapy with BCMA-directed CAR T cells has been documented, leading to fulminant recurrence of the disease [5]. Interestingly, antigen reduction under therapeutic selection pressure has so far not been reported for SLAMF7 [36]. Thus, we have generated a SLAMF7-directed CAR equipped with an antigen-binding domain derived from elotuzumab [15]. As SLAMF7 mediates myeloma cell adherence to the bone marrow, we hypothesized that SLAMF7 expression could be reduced in EMD and hence limit the efficacy of SLAMF7-directed therapy. Therefore, we retrospectively analyzed the efficacy of elotuzumab-based therapy, as well as expression levels of SLAMF7 in a small series of patients with RRMM and EMD. We show that elotuzumab had limited efficacy for the treatment of EMD; however, this was not due to reduced SLAMF7 expression levels as those were comparably high and consistent in all tissue specimens investigated. In our view, these data encourage the further development of SLAMF7-directed CAR T cells for the treatment of high-risk MM and phase I clinical evaluation is currently ongoing (NCT04499339).
Patients and methods
Patients
We performed a retrospective analysis at our tertiary care hospital of all patients with RRMM and EMD receiving an elotuzumab-based combination therapy from January 2016 to January 2020. We defined RRMM based on the current consensus recommendations of the International Myeloma Working Group (IMWG) [30]. Evidence of infiltration with aberrant plasma cells in tissue biopsies from extraosseous sites led to the diagnosis of EMD. All histopathological diagnoses were performed by expert hematopathologists. Radiological evaluation (CT, PET/CT, MRI) was used for the diagnosis of EMD, if tissue sampling was impossible. Retrospective analysis of clinical data and evaluation of SLAMF7 expression on myeloma samples were approved by the ethical committee of the Medical Faculty of the University of Wuerzburg, Germany (reference number: 61/18).
Assessment of response and outcome
We performed response assessment according to the IMWG Response Criteria [22]. According to the Kaplan–Meier method, PFS was calculated from initiation of elotuzumab-based treatment to confirmed serological or EMD progression or death attributed to disease progression, whichever occurred first; overall survival (OS) was calculated from initiation of elotuzumab-based treatment to death from any cause. The remaining patients were censored at the last date of follow-up. For statistical analyses, we used the Prism Software 7.0 (GraphPad, San Diego, CA).
Immunohistochemistry
Immunohistochemistry for SLAMF7 and CD138 was performed on formalin-fixed paraffin-embedded tissue slides (HPA055945/Sigma-Aldrich, dilution 1:1000 and MI15/DAKO, dilution 1:100, respectively) using the MEDAC HiDef Detection™ HRP Polymer System and DAB-2V (Histofine) according to the manufacturers’ instructions and standard immunohistochemical protocols. Normal tonsil specimens were used as control tissue.
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Results
Patient characteristics
From January 2016 to January 2020, we treated a total of 74 RRMM patients with an elotuzumab-based combination therapy, 15 (21%) of whom showed EMD. Only the latter were included for further analysis (Table 1). Within this collective, patients were diagnosed with MM at a median age of 62 (range 32-80) years and received elotuzumab-based therapy at a median of 4.5 (0.8-19) years after initial diagnosis. Approximately half of the patients were female (n = 8; 53%), the majority of the patients had initially presented with advanced disease (Salmon&Durie stage III, n = 12; 80%), and one-third of the patients had high-risk cytogenetics (n = 5; 33%) [27]. In half of the patients (n = 8; 53%), all detectable EMD lesions were adjacent to bone, while a minority had only EMD without any bone-adjacent lesions (n = 3; 20%) and the rest of the patients presented with a mixed picture (n = 4; 27%). Most frequent localizations of EMD manifestations were paravertebral (n = 12; 80%), soft tissues without adjacency to bone (n = 4; 27%), parenchymal organs, and lymph nodes (both n = 3; 20%). Prior to elotuzumab treatment, the patients had received a median of four (range 1-9) therapy lines. A majority of patients had previously undergone high-dose chemotherapy followed by autologous hematopoietic stem cell transplantation (n = 11; 73%). All patients had received treatment with bortezomib, and half of them had received treatment with a next-generation proteasome inhibitor (n = 8; 53%). All but one patient had been exposed to lenalidomide (n = 14; 93%), and half of them had additionally been exposed to one (n = 5; 33%) or two (n = 2, 13%) alternative immunomodulatory drugs (IMiDs). Almost half of the patients were refractory to their last line of therapy (n = 7; 47%).
Table 1
Clinical patient characteristics
Patients, n (%)
15 (100)
Age at dx, median (range)
62 (32-80)
Subtype, n (%)
IgG
11 (73)
IgA
2 (13)
LC
2 (13)
Salmon&Durie
I
2 (13)
II
1 (7)
IIIA
10 (67)
IIIB
2 (13)
Sex, n (%)
Female
8 (53)
Male
7 (47)
Cytogenetics, n (%)
High risk*
5 (33)
Standard risk
7 (47)
NA
3 (20)
Start of elotuzumab, median time from dx in months (range)
54 (10-228)
Prior lines of therapy, n (%)
1-3
7 (47)
4-6
5 (33)
> 6
3 (20)
Prior treatment, n (%)
Stem cell transplantation
11 (73)
Autologous
11 (73)
Allogeneic
3 (20)
Proteasome inhibitors
15 (100)
Bortezomib
15 (100)
Carfilzomib
7 (47)
Ixazomib
1 (7)
Immunomodulatory drugs
14 (93)
Thalidomide
3 (20)
Lenalidomide
14 (93)
Pomalidomide
6 (40)
Daratumumab
7 (47)
Localization of EMD
Paravertebral
12 (80)
Soft tissue without adjacency to bone
4 (27)
Parenchymal organ
3 (20)
Lymph nodes
3 (20)
Pleura
2 (13)
Skin
1 (7)
dx diagnosis; Ig immunoglobulin; LC light chain; NA not applicable; EMD extramedullary disease
*High risk as defined by the presence of del(17p) and/or t(4;14) and/or t(14;16) [27]
Treatment protocol
Two 4-week cycles of weekly elotuzumab applications (10 mg/kg body weight (BW) i.v.) were followed by fortnightly elotuzumab infusions (10 mg/kg BW) in combination with lenalidomide (n = 5; 33%), or monthly elotuzumab infusions (20 mg/kg BW) in combination with pomalidomide (n = 10; 67%). The IMiDs were administered orally throughout the first 3 weeks of each cycle at doses according to the treating physician’s choice. Dexamethasone was administered once weekly (20-40 mg). Treatment was continued until progression.
Response to therapy
In this cohort, patients received a median number of three (range 1-17) treatment cycles. Upon evaluation of the best serological response, the ORR was 40%, with one patient (7%) achieving a very good partial response and five patients (33%) achieving a partial response (PR). In five other patients (33%), we observed temporary stabilization of disease, while four patients (27%) were refractory to the elotuzumab-based therapeutic regimen.
Follow-up imaging was available for two-thirds of patients (n = 10; 67%). Regression or stable disease of the extramedullary lesions was noted in four patients (27%). Progressive EMD was observed in six patients (40%). Radiological and serological evaluation of response was consistent in most patients (n = 8; 53%). Two patients (13%) demonstrated better disease control on imaging than on serological evaluation: One individual achieved complete regression of the extramedullary lesion despite serological PR and another individual maintained PR on imaging despite continuous serological progression. At time of relapse, two patients (13%) experienced extramedullary progression despite ongoing serological response.
Upon survival analysis, the median PFS and OS in this cohort were 3.8 and 12.9 months, respectively (Fig. 1). The 1-year PFS and OS rates were 21% and 57%, respectively. Due to the limited number of patients in this study, we did not perform additional subgroup analyses.
Fig. 1
Progression-free survival (PFS, dashed line) and overall survival (OS, continuous line) of patients (n = 15). One-year PFS and OS are visualized by the dotted line
×
Expression of SLAMF7 on extramedullary myeloma cells prior to elotuzumab treatment
In search of reasons for the relatively poor response and outcome rates in the investigated patient collective, we considered reduced expression of SLAMF7 on the myeloma cells at extramedullary sites as a potential mechanism for limited elotuzumab efficacy. Thus, we aimed to investigate SLAMF7 protein expression on myeloma cells at extramedullary sites. We queried our database for patients who had undergone biopsies of EMD before initiation of elotuzumab treatment and identified a small number of patients (n = 3; 20%) for whom the EMD material was available in our tissue biobank. We further retrieved matching bone marrow biopsies from the respective individuals prior to elotuzumab therapy. Immunohistochemical stainings to evaluate SLAMF7 expression in CD138+ myeloma cells (Figs 2) documented strong and consistent SLAMF7 expression in all EMD and bone marrow biopsies (Table 2), indicating a preserved expression of the antigen in myeloma cells even when located outside the bone marrow niche. Interestingly, the clinical course under elotuzumab treatment was highly variable in these patients and PFS ranged from 1.3 to 9.4 months (Table 2).
Fig. 2
SLAMF7 expression on intramedullary and extramedullary myeloma cells prior to elotuzumab treatment. Giemsa (a, d) and immunohistochemical staining for CD138 (b, e) as well as SLAMF7 (c, f) in the bone marrow (a-c) and in a skin biopsy (d-f) obtained simultaneously prior to elotuzumab treatment (a-f: original magnification ×400; length of the scale bar in d: 50 μm). SLAMF7 shows a strong and consistent expression in the CD138-positive myeloma cells. VCD, bortezomib/cyclophosphamide/dexamethasone; HD-Mel/autoSCT, high-dose melphalan/autologous stem cell transplantation; Len-MT, lenalidomide maintenance; Dara, daratumumab; KRd, carfilzomib/lenalidomide/dexamethasone; BM, bone marrow; EMD, extramedullary disease; VTD-PACE, bortezomib/thalidomide/dexamethasone-cisplatin/adriamycin/cyclophosphamide/etoposide); EloPd, elotuzumab/pomalidomide/dexamethasone; BSC, best supportive care
Table 2
Clinical and immunohistochemical profile of patients with histologically confirmed extramedullary myeloma
Identifier
E1
E3#1
E8
E16
E17
E18#2
General
Age at dx
47
68
46
60
54
55
Sex
m
m
m
m
m
m
Subtype
λLC
IgGκ
IgGλ
IgGκ
IgAκ
IgGκ
S&D at dx
IIIB
IA
IIIA
IIIA
IIIB
IIIA
ISS at dx
2
1
ND
2
3
ND
FISH
Normal
t(4;14), +1q21
ND
del13q14, del1p32, +1q21
del17p, t(4;14), del13q14, +1q21
Normal
Previous lines of therapy
3
3
4
5
3
2
Prior SCT, type (number)
Auto (2)
Auto (2)
Auto (1)
allo (1)
Auto (2)
Auto (2)
Auto (3)
Prior IMiDs
Len
Thal, Len
Len
Len, Pom
Len, Pom
None
Prior PIs
Bort
Bort, Carf
Bort, Carf
Bort
Bort
Bort
Prior mAbs
None
Dara
Dara
None
None
None
EMD prior to elotuzumab
Yes
Yes
Yes
No
No
No
Elotuzumab treatment
Time from dx (months)
26
27
144
41
15
186
Combination IMiD
Pom
Pom
Pom
Pom
Len, Pom
Len
Treatment cycles
10
3
1
1
3
7
Best response
PR
PR
PD
SD
SD
PR
PFS (months)
9.4
2.9
1.3
0.9
2.7
9.0
OS (months)
12.9
4.4
5.4
1.0
4.7
>9.8#3
Type of progression
Serological
+ EMD
Serological + EMD
EMD
EMD
EMD
Serological + EMD
IHC
Localization of EMD
Paravertebral soft tissue
Skin
Paravertebral soft tissue
Maxillary mucosa
Lymph node
Thoracic wall
SLAMF7 on CD138+ EM MM
+++#4
+++
+++
+++
+++
+++
SLAMF7 on CD138+ BM MM
+++
+++
+++
+++
+++
+++
dx diagnosis; S&D Salmon&Durie; LC light chain; Ig immunoglobulin; ISS International Staging System; ND not done; FISH fluorescence in situ hybridization; SCT stem cell transplantation; auto autologous; allo allogeneic; IMiD immunomodulatory drug; Len lenalidomide; Thal thalidomide; Pom pomalidomide; PI proteasome inhibitor; Bort bortezomib; Carf carfilzomib; mAb monoclonal antibody; Dara daratumumab; EMD extramedullary disease; Elo elotuzumab; PR partial response; PD progressive disease; SD stable disease; PFS progression-free survival; OS overall survival; IHC immunohistochemistry; EM extramedullary; MM multiple myeloma; BM bone marrow
Expression of SLAMF7 on de novo extramedullary myeloma during elotuzumab treatment
A subset of patients in our study cohort developed additional or progressive EMD manifestations during elotuzumab therapy, but tissue biopsies from those lesions were not available. Thus, to investigate the expression of SLAMF7 on myeloma cells of EMD under therapeutic selection pressure, we screened our electronic database for patients with de novo EMD during elotuzumab treatment. We identified five (out of 59) patients who were newly diagnosed with EMD after initiation of elotuzumab treatment, but before the start of the subsequent treatment line. For three patients, tissue specimens of EMD biopsies were available. As baseline controls, we identified bone marrow biopsies from the respective patients prior to elotuzumab therapy. When we compared immunohistochemical stainings of SLAMF7 on bone marrow myeloma cells before treatment and on extramedullary myeloma cells after treatment (Fig. 3), we found strong and consistent SLAMF7 expression in all cases. This indicated that outgrowth of SLAMF7+ extramedullary myeloma occurred despite SLAMF7-directed selection pressure. The time span from initiation of elotuzumab treatment to diagnosis of EMD ranged from 1 to 7 treatment cycles and PFS of the respective patients ranged from 0.9 to 9.0 months (Table 2). In aggregate, SLAMF7 was strongly and consistently expressed on all bone marrow and EMD specimens of MM, irrespective of previous exposition and clinical response to anti-SLAMF7 targeted immunotherapy with elotuzumab.
Fig. 3
SLAMF7 expression on bone marrow myeloma cells prior to and on extramedullary myeloma cells after elotuzumab treatment. Giemsa (a, d) and immunohistochemical staining for CD138 (b, e) as well as SLAMF7 (c, f) in the bone marrow (a-c) and a biopsy from the chest wall (d-f) (a-f: original magnification ×400; length of the scale bar in d: 50 μm). The former was obtained prior to elotuzumab treatment, and the latter was obtained 9 months later, after 7 cycles of elotuzumab-based combination therapy. SLAMF7 shows a strong and consistent expression in the CD138-positive myeloma cells. IdaD, idarubicin/dexamethasone; HD-Mel/autoSCT, high-dose melphalan/autologous stem cell transplantation; IFNα-MT, interferon α maintenance; VCD, bortezomib/cyclophosphamide/dexamethasone; BM, bone marrow; EloRd, elotuzumab/lenalidomide/dexamethasone; EMD, extramedullary disease; BSC, best supportive care
×
Discussion
Monoclonal antibodies have become an important weapon in the therapeutic armamentarium against MM. However, their optimal incorporation into existing treatment paradigms remains to be defined, especially for patients with high-risk disease. To the best of our knowledge, this is the first study investigating the role of elotuzumab in EMD. While EMD patients were eligible for both elotuzumab approval trials [10, 23], EMD was removed as covariate from the regression analyses in the ELOQUENT-2 trial as consequence of reduced imaging interventions as per protocol amendment [23]. In ELOQUENT-3, lactate dehydrogenase (LDH) ≥ 300 U/l was used as surrogate parameter for EMD and subgroup analysis showed only moderate efficacy in this patient cohort with a hazard ratio of 0.75 (95% CI 0.31-1.84) [10]. However, Oka et al. reported a case of a patient with EMD resistant to lenalidomide/dexamethasone who responded to the addition of elotuzumab [26], whereas our group documented the occurrence of EMD during elotuzumab treatment in a small number of patients [8]. Taken together, the therapeutic value of elotuzumab for the treatment of EMD in patients with RRMM is unclear.
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In the present study, the elotuzumab-based regimen demonstrated limited efficacy with an ORR of 40% and a moderate outcome with a 1-year PFS rate of 21%, respectively. ELOQUENT-2 and ELOQUENT-3, by contrast, had documented ORRs of 79% and 53%, and 1-year PFS rates of 68% and 43%, respectively [10, 23]. One possible explanation for the observed limited efficacy in the herein investigated patient cohort might be an overrepresentation of patients having undergone a high number of previous lines of therapy (median of 4 vs. 2 and 3, respectively), including intensive pretreatment regimens (prior stem cell transplantation in 73% in the present study vs. 52% in both ELOQUENT trials). Furthermore, in our patient cohort, previous exposure to IMiDs was considerably higher than in ELOQUENT-2 (exposure to lenalidomide in 93% vs. 5%) and in our study, more patients had high-risk cytogenetics than in ELOQUENT-3 (33% vs. 22%).
As the presence of the respective antigen is an obligatory prerequisite for efficient targeted therapy, we hypothesized that another reason for the observed inferior outcome data in our cohort could be reduced SLAMF7 expression on myeloma cells located outside the bone marrow niche. Previous analyses of SLAMF7 expression in MM focused mainly on phenotypic characterization of myeloma cells originating from the bone marrow; however, Hsi et al. [19] demonstrated mostly preserved SLAMF7 levels on myeloma cells in a small number of EMD biopsies. Here, we observed strong and consistent SLAMF7 protein expression determined by immunohistochemistry in all investigated tissue specimens, including six EMD biopsies. Considering that a functional relevance of SLAMF7 for the homing of myeloma cells to the bone marrow was postulated [38], our findings suggest that SLAMF7 does not imperatively restrict myeloma cells to the bone marrow microenvironment. Furthermore, we did not identify primary absence of the target antigen as reason for reduced efficacy of elotuzumab in extramedullary lesions.
Downregulation or reduction of target antigens under selection pressure is a common mechanism of resistance in cancer cells [12, 14, 21, 24]. In MM, this phenomenon has been observed for CD38 during daratumumab treatment [25] as well as for BCMA during CAR T cell therapy [5]. To the best of our knowledge, data on SLAMF7 negative myeloma cells has not been published. Indeed, in our small cohort, we did not detect reduced antigen expression despite extramedullary spread under the anti-SLAMF7 selection pressure of the elotuzumab treatment. This might point towards either a relatively weak therapeutic pressure of elotuzumab or a relevant role of SLAMF7 for the maintenance of the disease. Given that SLAMF7 is a self-ligand, we cannot exclude that for extramedullary spread of single cells, e.g. into peritoneum, pleural cavity, or cerebrospinal fluid, accompanied by a disintegration of myeloma cell aggregates, SLAMF7 might become more dispensable for the persistence of the disease. This is in line with a previously reported case of EMD, where the malignant pleural effusion harbored myeloma cells with lower SLAMF7 expression than the bone marrow [8]. However, the evaluation of subtle differences in SLAMF7 expression levels requires analysis by flow cytometry or other methodologies which outperform immunohistochemistry with regard to antigen quantification.
Other potential reasons for the here observed limited efficacy of elotuzumab in EMD could result from the biology of extramedullary myeloma cell growth. Myeloma cells that are capable of surviving outside the bone marrow have previously undergone a biological evolution, including chromosomal aberrations, mutations of individual genes, and epigenetic changes [3]. Furthermore, they demonstrate an increased proliferative index compared to bone marrow myeloma cells [13, 31]. Therefore, it is plausible that the therapeutic efficacy of elotuzumab may be limited by the overall more aggressive nature of the disease in this setting. Another important factor to be considered is difference in the microenvironment in bone marrow and extramedullary myeloma niche, but unfortunately, the immunological composition and interactions of EMD have been studied poorly to date. However, preclinical observations suggest that microenvironment-dependent inhibitory mechanisms might impair the efficacy of immunotherapy at extramedullary myeloma sites [37]. Also, the high incidence of EMD after allogeneic stem cell transplantation [28, 32] could be interpreted as spread to more immune-deprived sites.
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We are aware of the limitations of the current study that are, in particular, related to its retrospective nature and the small sample size. However, we feel that such real-life data can provide valuable insights, especially for rare disease manifestations, and potentially guide the setup of prospective studies. In conclusion, our analyses demonstrate limited efficacy of current elotuzumab-based IMiD combination treatments in patients with EMD, despite strong and consistent presence of SLAMF7 on extramedullary myeloma cells. In our opinion, this observation encourages the further development of other potentially more potent SLAMF7-directed immunotherapies, such as antibody-drug conjugates [41] and CAR T cells [15].
Availability of data and material
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Code availability
Not applicable.
Declarations
Ethics approval
This study involving human participants was in accordance with the 1964 Helsinki Declaration and its later amendments and with the ethical standards of the institutional committee; the retrospective analysis of clinical data and evaluation of SLAMF7 expression on myeloma specimens were approved by the ethical committee of the Medical Faculty of the University of Wuerzburg (reference number: 61/18).
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Consent to participate
Informed consent was obtained from all individual participants prior to invasive tissue sampling. Informed consent was obtained from all patients of whom material was included in this study, or, if patients were retrospectively identified to be included in this study and had passed away in the meantime, informed consent was obtained from their next of kin.
Consent for publication
Not applicable.
Competing interests
The authors declare no competing interests.
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Elotuzumab for the treatment of extramedullary myeloma: a retrospective analysis of clinical efficacy and SLAMF7 expression patterns
verfasst von
Sophia Danhof Leo Rasche Anja Mottok Tabea Steinmüller Xiang Zhou Martin Schreder Teresa Kilian Susanne Strifler Andreas Rosenwald Michael Hudecek Hermann Einsele Elena Gerhard-Hartmann
