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Lasers in Medical Science

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23.08.2017 | Original Article

Enhanced survival of ischemic skin flap by combined treatment with bone marrow-derived stem cells and low-level light irradiation

verfasst von: Jeong Hwan Moon, Yun-Hee Rhee, Jin-Chul Ahn, Bongkyun Kim, Sang Joon Lee, Phil-Sang Chung

Erschienen in: Lasers in Medical Science | Ausgabe 1/2018

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Abstract

The aim of this study is to examine the enhanced survival effect of ischemic skin flap by combined treatment with bone marrow-derived stem cells (BMSCs) and low-level light irradiation (LLLI). The neovasculogenic effect of BMSCs induced by LLLI was detected using a wound healing and tube formation assay. ICR mice were divided into four groups: control group, LLLI group, BMSCs group, and combine-treated group. The percentage of skin flap necrosis area was calculated on the seventh post-operative day. Specimens were harvested for histologic analyses. LLLI promoted BMSC migration and tube formation. The flap survival rate of combined treated group was significantly higher than that of the control group. Histologic results demonstrated a significant increase in neovascularization in the combined treatment group. This study demonstrates that combination treatment of BMSCs and LLLI could enhance the survival of ischemic skin flap in a mouse model.

Risau W (1997) Mechanisms of angiogenesis. Nature 386:671–674CrossRefPubMed

Tepper OM, Galiano RD, Kalka C, Gurtner GC (2003) Endothelial progenitor cells: the promise of vascular stem cells for plastic surgery. Plast Reconstr Surg 111:846–854CrossRefPubMed

Lyden D, Hattori K, Dias S et al (2001) Impaired recruitment of bone-marrow-derived endothelial and hematopoietic precursor cells blocks tumor angiogenesis and growth. Nat Med 7:1194–1201CrossRefPubMed

Simman R, Craft C, McKinney B (2005) Improved survival of ischemic random skin flaps through the use of bone marrow nonhematopoietic stem cells and angiogenic growth factors. Ann Plast Surg 54:546–552CrossRefPubMed

Ichioka S, Kudo S, Shibata M, Ando J, Sekiya N, Nakatsuka T (2004) Bone marrow cell implantation improves flap viability after ischemia-reperfusion injury. Ann Plast Surg 52:414–418CrossRefPubMed

Corazza AV, Jorge J, Kurachi C, Bagnato VS (2007) Photobiomodulation on the angiogenesis of skin wounds in rats using different light sources. Photomed Laser Surg 25:102–106CrossRefPubMed

Kipshidze N, Nikolaychik V, Keelan MH et al (2001) Low-power helium: neon laser irradiation enhances production of vascular endothelial growth factor and promotes growth of endothelial cells in vitro. Lasers Surg Med 28:355–364CrossRefPubMed

Hou JF, Zhang H, Yuan X, Li J, Wei YJ, Hu SS (2008) In vitro effects of low-level laser irradiation for bone marrow mesenchymal stem cells: proliferation, growth factors secretion and myogenic differentiation. Lasers Surg Med 40:726–733CrossRefPubMed

Abramovitch-Gottlib L, Gross T, Naveh D et al (2005) Low level laser irradiation stimulates osteogenic phenotype of mesenchymal stem cells seeded on a three-dimensional biomatrix. Lasers Med Sci 20:138–146CrossRefPubMed

10.

Arnaoutova I, Kleinman HK (2010) In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract. Nat Protoc 5:628–635CrossRefPubMed

11.

Tang YH, Pennington LA, Scordino JW, Alexander JS, Lian T (2016) Dynamics of early stem cell recruitment in skin flaps subjected to ischemia reperfusion injury. Pathophysiology 23(3):221–228CrossRefPubMed

12.

Schweizer R, Kamat P, Schweizer D et al (2014) Bone marrow-derived mesenchymal stromal cells improve vascular regeneration and reduce leukocyte-endothelium activation in critical ischemic murine skin in a dose-dependent manner. Cytotherapy 16(10):1345–1360CrossRefPubMed

13.

Lamalice L, Le Boeuf F, Huot J (2007) Endothelial cell migration during angiogenesis. Circ Res 100:782–794CrossRefPubMed

14.

Maleki M, Ghanbarvand F, Reza Behvarz M, Ejtemaei M, Ghadirkhomi E (2014) Comparison of mesenchymal stem cell markers in multiple human adult stem cells. Int J Stem Cells 7:118–126CrossRefPubMedPubMedCentral

15.

Liu L, Gao J, Yuan Y, Chang Q, Liao Y, Lu F (2013) Hypoxia preconditioned human adipose derived mesenchymal stem cells enhance angiogenic potential via secretion of increased VEGF and bFGF. Cell Biol Int 37:551–560CrossRefPubMed

16.

Sun XT, Ding YT, Yan XG et al (2004) Angiogenic synergistic effect of basic fibroblast growth factor and vascular endothelial growth factor in an in vitro quantitative microcarrier-based three-dimensional fibrin angiogenesis system. World J Gastroenterol: WJG 10:2524–2528CrossRefPubMedPubMedCentral

17.

Yang M, Sheng L, Li H, Weng R, Li QF (2010) Improvement of the skin flap survival with the bone marrow-derived mononuclear cells transplantation in a rat model. Microsurgery 30:275–281CrossRefPubMed

18.

Cury V, Moretti AI, Assis L et al (2013) Low level laser therapy increases angiogenesis in a model of ischemic skin flap in rats mediated by VEGF, HIF-1alpha and MMP-2. J Photochem Photobiol B 125:164–170CrossRefPubMedPubMedCentral

19.

Uysal AC, Mizuno H, Tobita M, Ogawa R, Hyakusoku H (2009) The effect of adipose-derived stem cells on ischemia-reperfusion injury: immunohistochemical and ultrastructural evaluation. Plast Reconstr Surg 124:804–815CrossRefPubMed

20.

Prado RP, Liebano RE, Hochman B, Pinfildi CE, Ferreira LM (2006) Experimental model for low level laser therapy on ischemic random skin flap in rats. Acta cirurgica brasileira/Sociedade Brasileira para Desenvolvimento Pesquisa em Cirurgia 21:258–262CrossRef

21.

Briers JD, Fercher AF (1982) Retinal blood-flow visualization by means of laser speckle photography. Invest Ophthalmol Vis Sci 22:255–259PubMed

22.

Boas DA, Dunn AK (2010) Laser speckle contrast imaging in biomedical optics. J Biomed Opt 15:011109CrossRefPubMedPubMedCentral

23.

Lu F, Mizuno H, Uysal CA, Cai X, Ogawa R, Hyakusoku H (2008) Improved viability of random pattern skin flaps through the use of adipose-derived stem cells. Plast Reconstr Surg 121:50–58CrossRefPubMed

24.

Horvat-Karajz K, Balogh Z, Kovacs V, Drrernat AH, Sreter L, Uher F (2009) In vitro effect of carboplatin, cytarabine, paclitaxel, vincristine, and low-power laser irradiation on murine mesenchymal stem cells. Lasers Surg Med 41:463–469CrossRefPubMed

25.

Keck PJ, Hauser SD, Krivi G et al (1989) Vascular permeability factor, an endothelial cell mitogen related to PDGF. Science 246:1309–1312CrossRefPubMed

26.

Folkman J, Klagsbrun M (1987) Angiogenic factors. Science 235:442–447CrossRefPubMed

27.

Suri C, Jones PF, Patan S et al (1996) Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis. Cell 87:1171–1180CrossRefPubMed

28.

Yang EY, Moses HL (1990) Transforming growth factor beta 1-induced changes in cell migration, proliferation, and angiogenesis in the chicken chorioallantoic membrane. J Cell Biol 111:731–741CrossRefPubMed

Titel: Enhanced survival of ischemic skin flap by combined treatment with bone marrow-derived stem cells and low-level light irradiation
verfasst von: Jeong Hwan Moon
Yun-Hee Rhee
Jin-Chul Ahn
Bongkyun Kim
Sang Joon Lee
Phil-Sang Chung
Publikationsdatum: 23.08.2017
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 1/2018
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-017-2312-9

Springer Medizin

Abstract

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