Skip to main content

01.01.2005 | Original Investigation | Ausgabe 1-2/2005

Medical Microbiology and Immunology 1-2/2005

Enhancement of antimicrobial effects by glucocorticoids

Medical Microbiology and Immunology > Ausgabe 1-2/2005
Jochen Türck, Claudia Oberdörfer, Tanja Vogel, Colin R. MacKenzie, Walter Däubener


In the past few years a body of evidence has accumulated showing that stimulation of human astrocytes and microvascular endothelial cells with IFN-γ induces a potent antibacterial and anti-parasitic effect. We have found that the IFN-γ-mediated activation of the tryptophan-degrading enzyme indoleamine 2,3-dioxygenase (IDO) is, at least in part, responsible for this antimicrobial activity. Glucocorticoids are frequently used in inflammatory central nervous system diseases to reduce the inflammatory reaction and cerebral edema. Since in many inflammatory conditions infection is either a primary or secondary factor, steroids are administered, in these circumstances, during infection. We investigated whether steroids could affect the antimicrobial effect of IFN-γ-induced IDO activation. We found that hydrocortisone and dexamethasone enhance IFN-γ-mediated IDO activity in both human astrocytoma cells and native human astrocytes. Furthermore, we found that the amounts of IDO mRNA and of IDO protein are enhanced in cells treated with IFN-γ and glucocorticoids. In addition, we were able to demonstrate that both steroids enhance the IFN-γ-mediated antimicrobial activity against Toxoplasma gondii, Staphylococcus aureus and group B streptococci. The enhanced antimicrobial effect of IFN-γ in the presence of glucocorticoids is due to the enhancement of the IDO-mediated tryptophan degradation, demonstrated by the complete abrogation of this antimicrobial effect by tryptophan resupplementation. These data show that glucocorticoids, which were often used to inhibit proinflammatory processes, do not decrease IDO-mediated antimicrobial effects. In contrast, high doses of steroids were able to enhance the IFN-γ-induced antimicrobial activity.

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

e.Med Interdisziplinär

Mit e.Med Interdisziplinär erhalten Sie Zugang zu allen CME-Fortbildungen und Fachzeitschriften auf

Jetzt e.Med zum Sonderpreis bestellen!

Sichern Sie sich jetzt Ihr e.Med-Abo und sparen Sie 50 %!

Über diesen Artikel

Weitere Artikel der Ausgabe 1-2/2005

Medical Microbiology and Immunology 1-2/2005 Zur Ausgabe