Background
Methods
Patients
Microbiology
Virus | Primer | Polarity | Sequence(5′-3′) | Product size (bp) | Reference |
---|---|---|---|---|---|
Group B rotavirus | B5-2 | + | GGCAATAAAATGGCTTCATTGC | 814 | [13] |
B5-3 | - | GGGTTTTTACAGCTTCGGCT | |||
GroupC rotavirus | NG8S1 | + | ATTATGCTCAGACTATCGCCAC | 352 | [13] |
NG8S2 | - | GTTTCTGTACTAGCTGGTGAAC | |||
Adenovirus | Ad1 | + | TTCCCCATGGCICAYAACAC | 482 | [13] |
Ad2 | - | CCCTGGTAKCCRATRTTGTA | |||
Norovirus (GI) | G1-SKF | + | CTGCCCGAATTYGTAAATGA | 330 | [14] |
GI-SKR | - | CCAACCCARCCATTRTACA | |||
Norovirus (GII) | CoG2F | + | CARGARBCNATGTTYAGRTGGATGAG | 387 | [14] |
G2-SKR | - | CCRCCNGCATRHCCRTTRTACAT | |||
Sapovirus | SLV-5317 | + | CTCGCCACCTACRAWGCBTGGTT | 434 | [14] |
SLV-5749 | - | CGGRCYTCAAAVSTACCBCCCCA | |||
Astrovirus | PreCAP1 | + | GGACTGCAAAGCAGCTTCGTG | 719 | [14] |
82b | - | GTGAGCCACCAGCCATCCCT |
Characterization of diarrheagenic E. coli by multiplex PCR
Antimicrobial susceptibility testing
Data analysis
Results
Characteristic | No. of case (%) |
---|---|
Seasons | |
Spring | 446(19.2) |
Summer | 855(36.9) |
Fall | 414(17.9) |
Winter | 603(26.0) |
Age (years) | |
< 1 | 1013(43.7) |
1–2 | 711(30.7) |
3–5 | 594(25.6) |
Mean age ± SD | 1.17 ± 1.23 |
Sex ratio (M/F) | 1.72/1 |
Diarrhea | |
Frequency | 4.17 ± 2.43 |
Loose | 772(33.3) |
Mucus | 248(10.7) |
Watery | 361(15.6) |
Bloody | 13(0.5) |
Unknown | 924(39.9) |
Vomiting | 781(32.5) |
Frequency | 2.28 ± 1.71 |
Watery | 57(2.7) |
Stomach contents | 724(29.8) |
Fever | 212(8.9) |
Abdominal pain | 147(6.0) |
Antibiotic consumption | 114(4.4) |
Pathogen | No. (%) of isolates in patients | No. (%) of isolates in patients of the following ages |
p value | No. (%) of isolates in patients of the following seasons |
p value | |||||
---|---|---|---|---|---|---|---|---|---|---|
<1 year (n = 1013) | 1–2 years (n = 711) | 2–5 years (n = 594) | Spring (n = 446) | Summer (n = 855) | Fall (n = 414) | Winter (n = 603) | ||||
Rotavirus | 443(19.1) | 184(18.2) | 170(23.9) | 89(15.0) |
0.000
| 96(21.5) | 81(9.5) | 69(16.7) | 197(32.7) |
0.000
|
Calicivirus | 411(17.7) | 151(14.9) | 123(17.3) | 137(23.1) |
0.000
| 101(22.6) | 130(15.2) | 61(14.7) | 119(19.7) |
0.002
|
Norovirus I | 52(2.2) | 14(1.4) | 15(2.1) | 23(3.9) |
0.005
| 4(0.9) | 18(2.1) | 16(3.9) | 14(2.3) |
0.033
|
Norovirus II | 320(13.8) | 127(12.5) | 93(13.1) | 100(16.8) |
0.044
| 90(20.2) | 101(11.8) | 41(9.9) | 88(14.6) |
0.000
|
Sappovirus | 39(1.7) | 10(1.0) | 15(2.1) | 14(2.4) | 0.068 | 7(1.6) | 11(1.3) | 4(1.0) | 17(2.8) | 0.077 |
Astrovirus | 38(1.6) | 13(1.3) | 12(1.7) | 13(2.2) | 0.383 | 9(2.0) | 9(1.1) | 5(1.2) | 15(2.5) | 0.145 |
Adenovirus | 51(2.2) | 22(2.2) | 24(3.4) | 5(0.8) |
0.008
| 9(2.0) | 11(1.3) | 14(3.4) | 17(2.8) | 0.067 |
Diarrheagenic E. coli
| 177(7.6) | 6 (6.0) | 43(6.0) | 73(12.3) |
0.000
| 24(5.4) | 98(11.5) | 21(5.1) | 34(5.6) |
0.000
|
EAEC | 104(4.5) | 39(3.8) | 22(3.1) | 43(7.2) |
0.001
| 14(3.1) | 62(7.3) | 7(1.7) | 21(3.5) |
0.000
|
EPEC | 40(1.7) | 8(0.8) | 13(1.8) | 19(3.2) |
0.002
| 5(1.1) | 19(2.2) | 9(2.2) | 7(1.2) | 0.281 |
ETEC | 24(1.0) | 7(0.7) | 6(0.8) | 11(1.9) | 0.071 | 5(1.1) | 11(1.3) | 2(0.5) | 6(1.0) | 0.651 |
EHEC | 7(0.3) | 5(0.5) | 2(0.3) | 0(0) | NA | 0(0) | 4(0.5) | 3(0.7) | 0(0) | NA |
EIEC | 2(0.1) | 2(0.2) | 0 0) | 0 0) | NA | 0 0) | 2 0.2) | 0 0) | 0(0) | NA |
Shigella spp. | 34(1.5) | 15(1.5) | 12(1.7) | 7(1.2) | 0.747 | 4(0.9) | 21(2.5) | 7(1.7) | 2(0.3) |
0.006
|
S. flexneri
| 2(0.1) | 0(0) | 0(0) | 2(0.3) | NA | 0(0) | 2(0.2) | 0(0) | 0(0) | NA |
S. sonnei
| 26(1.1) | 11(1.1) | 10(1.4) | 5(0.8) | 0.621 | 4(0.9) | 18(2.1) | 3(0.7) | 1(0.2) |
0.004
|
S. boydii
| 6(0.3) | 4(0.4) | 2(0.3) | 0(0) | NA | 0(0) | 1(0.1) | 4(1.0) | 1(0.2) | NA |
Vibrio parahaemolyticus
| 29(1.3) | 10(1.0) | 6(0.8) | 13(2.2) | 0.056 | 2(0.4) | 16(1.9) | 7(1.7) | 4(0.7) | 0.062 |
Salmonella spp. | 15(0.6) | 4(0.4) | 2(0.3) | 9(1.5) |
0.009
| 2(0.4) | 9(1.1) | 2(0.5) | 2(0.3) | 0.312 |
Campylobacter jejuni
| 10(0.4) | 5(0.5) | 2(0.3) | 3(0.5) | 0.764 | 0(0) | 5(0.6) | 3(0.7) | 2(0.3) | NA |
Plesiomonas shigelloides
| 9(0.4) | 2(0.2) | 2(0.3) | 5(0.8) | 0.115 | 2(0.4) | 5(0.6) | 2(0.5) | 0(0) | NA |
Vibrio cholerae
| 6(0.3) | 4(0.4) | 0(0) | 2(0.3) | NA | 0(0) | 3(0.4) | 0(0) | 3(0.5) | NA |
Aeromonas hydrophila
| 5(0.2) | 2(0.2) | 0(0) | 3(0.5) | NA | 0(0) | 5(0.6) | 0(0) | 0(0) | NA |
Yersinia enterocolitica
| 4(0.2) | 5(0.5) | 0(0) | 0(0) | NA | 1(0.2) | 3(0.4) | 0(0) | 0(0) | NA |
Campylobacter spp. | 3(0.1) | 1(0.1) | 0(0) | 2(0.3) | NA | 0(0) | 0(0) | 2(0.5) | 1(0.2) | NA |
Amoeba
| 14(0.6) | 1(0.1) | 2(0.3) | 10(1.7) |
0.000
| 2(0.4) | 8(0.9) | 4(1.0) | 0(0) | NA |
Giardia lamblia
| 2(0.1) | 0(0) | 2(0.3) | 0(0) | NA | 0(0) | 2(0.2) | 0(0) | 0(0) | NA |
Cryptosporidiosis
| 2(0.1) | 0(0) | 0(0) | 2(0.3) | NA | 0(0) | 0(0) | 2(0.5) | 0(0) | NA |
Patterns of infection | No. of case (%) |
---|---|
Rotavirus + Calicivirus | 121(5.2) |
Rotavirus + Calicivirus + Diarrheagenic E. coli
| 21(0.9) |
Rotavirus + Diarrheagenic E. coli
| 19(0.8) |
Calicivirus + Diarrheagenic E. coli
| 14(0.6) |
Rotavirus + Adenovirus | 12(0.5) |
Rotavirus + Astrovirus | 11(0.5) |
Rotavirus + Calicivirus + Adenovirus | 9(0.4) |
Calicivirus + Adenovirus | 7(0.3) |
Calicivirus + Astrovirus | 7(0.3) |
Rotavirus + Calicivirus + Astrovirus | 5(0.2) |
Rotavirus + Vibrio parahaemolyticus | 3(0.1) |
Calicivirus + Salmonella spp. | 3(0.1) |
Calicivirus + Shigella spp. | 2(0.1) |
Salmonella spp. + Vibrio cholerae
| 2(0.1) |
Calicivirus + Vibrio parahaemolyticus
| 2(0.1) |
Salmonella spp. + Yersinia intestinal colon | 1(0.0) |
Vibrio parahaemolyticus + Vibrio cholerae
| 1(0.0) |
Rotavirus + Calicivirus + Astrovirus + Adenovirus | 1(0.0) |
Calicivirus + Adenovirus + Shigella spp. | 1(0.0) |
Diarrheagenic E. coli + Campylobacter jejuni + Yersinia enterocolitica
| 1(0.0) |
Rotavirus + Adenovirus + Diarrheagenic E. coli
| 1(0.0) |
Calicivirus + Diarrheagenic E. coli + Campylobacter jejuni
| 1(0.0) |
Rotavirus + Calicivirus + Yersinia enterocolitica
| 1(0.0) |
Rotavirus + Calicivirus + Astrovirus + Diarrheagenic E. coli
| 1(0.0) |
Antimicrobial | DEC | EAEC | ETEC | EPEC | STEC |
P
a
| |||||
---|---|---|---|---|---|---|---|---|---|---|---|
(n = 177) | (n = 104) | (n = 40) | (n = 24) | (n = 7) | |||||||
%R | %S | %R | %S | %R | %S | %R | %S | %R | %S | ||
AMP | 93.2 | 2.8 | 96.2 | 1.9 | 95.0 | 0 | 83.3 | 4.2 | 71.4 | 28.6 | 0.055 |
TCY | 60.0 | 38.3 | 61.5 | 38.5 | 55.0 | 37.5 | 79.2 | 20.8 | 0 | 100 | 0.258 |
CZO | 57.7 | 26.9 | 55.8 | 27.9 | 55.0 | 27.5 | 79.2 | 20.8 | 28.6 | 28.6 | 0.093 |
SXT | 57.2 | 39.5 | 63.5 | 33.7 | 55.0 | 37.5 | 50.0 | 50.0 | 0 | 100 | 0.467 |
PIP | 51.4 | 32.5 | 49.0 | 31.7 | 55.0 | 27.5 | 62.5 | 33.3 | 28.6 | 71.4 | 0.209 |
CXM | 46.3 | 50.3 | 51.0 | 49.0 | 37.5 | 55.0 | 45.8 | 54.2 | 28.6 | 28.6 | 0.347 |
SAM | 45.1 | 37.1 | 42.3 | 36.5 | 55.0 | 37.5 | 45.8 | 29.2 | 28.6 | 71.4 | 0.392 |
CTX | 42.3 | 54.9 | 49.0 | 51.0 | 27.5 | 62.5 | 41.7 | 54.2 | 28.6 | 71.4 | 0.064 |
GEN | 35.4 | 60.0 | 36.5 | 59.6 | 37.5 | 55.0 | 37.5 | 58.3 | 0 | 100 | 0.992 |
ATM | 21.7 | 68.6 | 27.9 | 63.5 | 10.0 | 90.0 | 20.8 | 54.2 | 0 | 71.4 | 0.070 |
CIP | 17.7 | 79.4 | 23.1 | 72.1 | 7.5 | 92.5 | 16.7 | 83.3 | 0 | 100 | 0.095 |
CAZ | 14.8 | 85.2 | 19.2 | 80.8 | 10.0 | 90.0 | 8.3 | 91.7 | 0 | 100 | 0.226 |
AMC | 12.0 | 69.1 | 14.4 | 72.1 | 10.0 | 62.5 | 0.0 | 66.7 | 28.6 | 71.4 | 0.127 |
FEP | 11.4 | 76.5 | 14.4 | 74.0 | 10.0 | 72.5 | 4.2 | 95.8 | 0 | 71.4 | 0.343 |
FOX | 5.1 | 93.7 | 6.7 | 91.3 | 0 | 100 | 0 | 100 | 28.6 | 71.4 | 0.106 |
TZP | 4.0 | 90.9 | 6.7 | 85.6 | 0 | 100 | 0 | 95.8 | 0 | 100 | 0.106 |
CSL | 2.3 | 74.3 | 3.8 | 72.1 | 0 | 72.5 | 0 | 79.2 | 0 | 100 | 0.283 |
AMK | 1.1 | 93.7 | 1.9 | 93.3 | 0 | 90.0 | 0 | 100 | 0 | 100 | 0.536 |
IPM | 0 | 100 | 0 | 100 | 0 | 100 | 0 | 100 | 0 | 100 | NAb
|
MEM | 0 | 100 | 0 | 100 | 0 | 100 | 0 | 100 | 0 | 100 | NAb
|