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01.12.2012 | Research | Ausgabe 1/2012 Open Access

Malaria Journal 1/2012

Entomologic investigation of Plasmodium knowlesi vectors in Kuala Lipis, Pahang, Malaysia

Zeitschrift:
Malaria Journal > Ausgabe 1/2012
Autoren:
Adela I Jiram, Indra Vythilingam, Yusuf M NoorAzian, Yusri M Yusof, Abdul H Azahari, Mun-Yik Fong
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1475-2875-11-213) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

IV conceived the study. AIJ, IV and FMY were responsible for the preparation of the manuscript. IV, AIJ, NAY, YY and AAH were responsible for field collection, supervision, identification and processing of mosquitoes. AIJ and IV were responsible for the molecular work. AIJ, FMY and IV analysed sequence data. All authors have read and approved the manuscript.

Abstract

Background

The first natural infection of Plasmodium knowlesi in humans was recorded in 1965 in peninsular Malaysia. Extensive research was then conducted and it was postulated that it was a rare incident and that simian malaria will not be easily transmitted to humans. However, at the turn of the 21st century, knowlesi malaria was prevalent throughout Southeast Asia and is life threatening. Thus, a longitudinal study was initiated to determine the vectors, their seasonal variation and preference to humans and macaques.

Methods

Monthly mosquito collections were carried out in Kuala Lipis, Pahang, peninsular Malaysia, using human-landing collection and monkey-baited traps at ground and canopy levels. All mosquitoes were identified and all anopheline mosquitoes were dissected and the gut and gland examined for oocysts and sporozoites. Nested polymerase chain reaction (PCR) was conducted on positive samples, followed by sequencing of the csp gene.

Results and discussion

Anopheles cracens was the predominant mosquito biting humans as well as the macaques. It comprised 63.2% of the total collection and was the only species positive for sporozoites of P. knowlesi. It was exophagic and did not enter houses. Besides An. cracens, Anopheles kochi was also found in the monkey-bait trap. Both species preferred to bite monkeys at ground level compared to canopy.

Conclusion

Anopheles cracens, which belongs to the Dirus complex, Leucosphyrus subgroup, Leucosphyrus group of mosquitoes, has been confirmed to be the only vector for this site from Pahang during this study. It was the predominant mosquito at the study sites and with deforestation humans and villages are entering deeper in the forests, and nearer to the mosquitoes and macacques. The close association of humans with macaques and mosquitoes has led to zoonotic transmission of malaria.
Zusatzmaterial
Authors’ original file for figure 1
12936_2012_2176_MOESM1_ESM.pdf
Authors’ original file for figure 2
12936_2012_2176_MOESM2_ESM.pdf
Authors’ original file for figure 3
12936_2012_2176_MOESM3_ESM.pdf
Literatur
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