Listeria monocytogenes as the main causative agent of human listeriosis is an intracellular bacterium that has the capability to infect a wide range of cell types. Human listeriosis is a sporadic foodborne disease, which is epidemiologically linked with consumption of contaminated food products. Listeriosis may range from mild and self-limiting diseases in healthy people to severe systemic infections in susceptible populations. This study aimed to investigate the prevalence of L. monocytogenes in food resources and human samples from Iran.
Methods
A systematic search was performed by using electronic databases from papers that were published by Iranian authors Since January of 2000 to the end of April 2017. Then, 47 publications which met our inclusion criteria were selected for data extraction and analysis by Comprehensive Meta-Analysis Software.
Results
The pooled prevalence of L. monocytogenes in human origin was 10% (95% CI: 7–12%) ranging from 0 to 28%. The prevalence of L. monocytogenes in animals was estimated at 7% (95% CI: 4–10%) ranging from 1 to 18%. Moreover, the pooled prevalence of L. monocytogenes in Iranian food samples was estimated at 4% (95% CI: 3–5%) ranging from 0 to 50%. From those 12 studies which reported the distribution of L. monocytogenes serotypes, it was concluded that 4b, 1/2a, and 1/2b were the most prevalent serotypes.
Conclusions
The prevalence of L. monocytogenes and prevalent serotypes in Iran are comparable with other parts of the world. Although the overall prevalence of human cross-contamination origin was low, awareness about the source of contamination is very important because of the higher incidence of infections in susceptible groups.
Preferred Reporting Items for Systematic Reviews and Meta-Analyses
Background
Listeria is ubiquitous Gram-positive bacteria, which are rod-shaped, facultative anaerobic, and non-spore forming, with a low C + G content [1]. The genus Listeria is composed of several species, of which Listeria monocytogenes is an opportunistic pathogen of humans and animals [1]. Due to ubiquitous nature of Listeria spp., and their unique ability to survive across a broad range of environmental stress including pH, temperature, and salt, they are considered as important foodborne pathogens [2].
L. monocytogenes as the main causative agent of human listeriosis is an intracellular bacterium that has the capability to infect a wide range of cell types and cross the intestinal, blood-brain and placental barriers [3]. Human listeriosis is a sporadic foodborne disease, which is epidemiologically linked with consumption of contaminated food products [4]. In human, listeriosis may range from a mild and self-limiting flu-like sickness or febrile gastroenteritis in healthy people to severe systemic infections including meningitis, septicemia, and abortion in susceptible people [3]. High-risk individuals are the pregnant women, neonates, elderly, immunocompromised individuals and adults with malignancy [5]. Listeriosis can be a serious disease with an approximate 20% mortality; that case–fatality rate may increase in groups at highest risk [4]. Regarding the wide distribution of L. monocytogenes in food resource and high fatality rate of listeriosis, L. monocytogenes has been considered as a major public health concern [1].
Anzeige
Variation in Iranian food tastes results in consumption of different kinds of foods, which may be considered as a risk factor of listeriosis outbreaks. Despite some local information on the prevalence of Listeria spp. in various food resources in Iran, there is no comprehensive data available on its prevalence to estimate the burden of L. monocytogenes. Therefore, this study aimed to investigate the prevalence of L. monocytogenes in food resources and human samples from Iran by using a systematic review and meta-analysis based method. This finding can provide good epidemiological background contributing to the international data of L. monocytogenes distribution.
Methods
Search strategies
A systematic literature search was conducted in the Web of Science, PubMed, Scopus and Google Scholar electronic databases from papers that were published by Iranian authors since January of 2000 to the end of April 2017. The following terms, “Listeriosis” or “Listeria” or “L. monocytogenes”, in combination with “Food”, “Animal”, “Human”, and “Iran” were searched as scientific keywords in the present survey both separately and simultaneously in March and April 2017.
Selection criteria and quality assessment
Two reviewers independently screened the databases with the related keywords and reviewed the titles, abstracts, and full texts to determine the articles which met the inclusion criteria; any discrepancies were resolved by consensus. The articles published in English or Persian language with English abstract which indexed in Pubmed or Scopus and had met the inclusion criteria were considered in our survey: standard methods (Culture methods, the results based on antibodies (ELISA) and molecular techniques) were used for Listeria detection, present data on the prevalence of L. monocytogenes, and samples were collected from foods or clinical samples. The criteria for identifying Iranian authors were the author or location of the work and also affiliations of authors. Additionally, research that has been conducted by non-Iranian authors on the Iranian population or samples were also assessed. Studies that did not use standardized methods, the sample size was less than 10 isolates, duplicate reports, and articles, samples obtained from environment sources or the origin of samples was unclear in them, articles that were written in Persian with Persian abstract and studies which did not detect L. monocytogenes were excluded. The quality of eligible studies was judged independently by two authors in accordance with the Joanna Briggs Institute. Eventually, the studies that obtained more than 60% were included in this study [6].
Data extraction
The following details were extracted for each of the included studies: the first author’s name, the time of performing the study, publication date, the study setting, sample size, source of isolation, the frequency of Listeria spp., and L. monocytogenes serotypes.
Anzeige
Statistical analysis
To estimate the overall prevalence meta-analyses, “metaprop program” in STATA version 14.0 (STATA, College Station, TX, USA) statistical software was used [7]. Meta-analysis was performed by using the random-effects model to estimate the pooled prevalence and corresponding 95% confidence interval (CI). Statistical heterogeneity groups were estimated using the Cochran Chi-square test and the Cochrane-I2. The funnel plot, Begg’s rank correlation test, and Egger’s weighted regression tests were used to evaluate possible publication bias (P < 0.05 was considered as an indication of a statistically significant publication bias). Possible sources of heterogeneity were evaluated by sensitivity analysis, meta-regression and subgroup analysis based on the location of the study and diagnostic methods [8, 9]. Sensitivity analysis was applied to determine that the exclusion of any study has a significant effect on the estimated pooled prevalence while ignoring each individual one. The present study designed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines (Additional file 1).
Results
The database search yielded 4990 citations. Among them, 4931 were removed by index, title and abstract screening and 59 were accessed in full text. Of 59 reviewed studies, three studies had a sample size less than 10 isolates, three studies did not report the prevalence of L. monocytogenes, two studies had a methodological problem, two studies collected samples from environment sources, and results of two studies were unclear. Finally, 47 studies matched with eligibility criteria and were subjected to meta-analysis, [2‐4, 10‐53]. However, out of 47 included studies, three studies reported prevalence in animals and/ humans and/food, simultaneously. The searching procedure for selection of eligible studies is demonstrated in Fig. 1.
×
The full results of the included articles, sample size, the prevalence of L. monocytogenes and predominant serotypes are presented in Table 1.
Table 1
Characteristics of studies included in the meta-analysis
Author
Publication year
Years of study
City or Province/Region
Diagnostic method
Sample source
Types food/animal species/Human
Study design
Sample size
L. monocytogenes
Predominant serotypes
L. innocua
Total
Ref
Firouzi et al.
2000
UN
Shiraz/South
Culture
Human
Slaughter houses
Cross sectional
130
0
–
0
3
6
Akhondzadeh Basti et al.
2004
UN
Tehran and Guilan/North
Culture
Food
Fresh fish, salted and smoked fish
Cross sectional
120
4
4b
–
–
7
Moshtaghi et al.
2007
2005
Shahrekord/Central
Culture
Food
Raw milk
Cross sectional
500
8
4b
3
11
8
Rahimi et al.
2008
2006–2007
Isfahan/Central
Culture
Animal
Cattle
Cross sectional
200
6
–
–
–
9
Jalali et al.
2008
2003–2005
Isfahan/ Central
PCR
Food
Meat, diary, vegetables products ready to eat food
Cross sectional
461
7
–
13
27
4
Jamshidi et al.
2009
2002–2003
Bandar Abbas/South
Serology
Human
‘Spontaneous abortion, control group
Case-control study
450
124
–
–
–
10
Jami et al.
2010
2008
Mashhad/Northeast
PCR
Food
Raw milk
Cross sectional
100
4
–
–
–
11
Rahimi et al.
2010
2007–2009
Isfahan/Central
PCR
Food
Milk,dairy products
Cross sectional
594
18
–
32
55
12
Mahmoodi et al.
2010
UN
Noorabad/South
Culture
Food
Raw milk, White cheese, yoghurt
Cross sectional
360
6
–
–
–
13
Lotfollahi et al.
2011
2009–2010
Tehran/North
Culture
Human
Spontaneous abortions
Cross sectional
100
9
–
–
–
14
Rahimi et al.
2011
UN
Tehran/North
Culture
Human
Pregnant mothers
Cross sectional
512
5
–
–
–
15
Rahimi et al.
2011
2009–2010
Isfahan and Shahrekord/Central
PCR
Food
Sea food
Cross sectional-
264
5
–
15
20
16
Ghasemian Safaei et al.
2011
2008
Shahrekord/Central
Culture
Food
Eggs
Cross sectional
100
0
–
–
–
17
Goudarzi et al.
2012
2011
Karaj/North
PCR
Human
Women with septic abortion
Cross sectional
87
12
–
–
–
18
Fallah et al.
2012
2010–2011
Shahrekord/Central
PCR
Food
Poultry product
Cross sectional
402
52
4b, 1/2a, 1/2b, 1/2c
62
134
2
Zarei et al.
2012
UN
Ahvaz/Southwest
PCR
Food
Raw/fresh,frozen, and ready-to-eat (RTE) seafood
Cross sectional
245
2
–
–
–
19
Hosseinzadeh et al.
2012
2009
Shiraz/South
PCR
Animal
Poultry flocks
Cross sectional
100
7
–
–
–
20
Safarpoor Dehkordi et al.
2012
2011
Various parts of Iran
Real-Time PCR
Food
Milk
Cross sectional-
596
69
–
–
–
21
Animal
Vaginal swab/Urine samples
1575
158
Rahimi et al.
2012
2009–2010
Chahar Mahal & Bakhtiyari/Central and South
Culture and PCR
Food
Dairy products
Cross sectional
290
5
–
14
21
22
Rahimi et al.
2012
2010–2011
Various parts of Iran
PCR
Food
Raw meats
Cross sectional
1107
27
–
98
141
23
Seifi et al.
2012
2009–2010
North and west
PCR
Animal
Broiler fl ocks
Cross sectional
490
44
–
–
–
24
Fallah et al.
2013
2011–2012
Shahrekord/ Central
PCR
Food
Raw and RTE seafood product
Cross sectional
462
35
1/2a, 4b, 1/2c, 1/2b, 4c
–
–
25
Jamali et al.
2013a
2008–2010
Tehran/North
PCR
Food
Raw milk
Cross sectional
446
18
1/2a, 3a; 1/2c, 3c; 4b, 4d, 4e
48
83
26
Jamali et al.
2013b
2008–2010
Tehran/North
PCR
Food
Milk
Cross sectional
207
17
(4b, 4d or 4e), (1/2a or 3a), (1/2b, 3b or 7), (1/2c or 3c)
3
21
27
Sohrabi et al.
2013
UN
Isfahan/Central
Culture
Food
Poultry meat
Cross sectional
52
1
–
11
12
28
Vahedi et al.
2013
2011
Sari/North
Culture
Food
Milk
Cross sectional
200
0
–
–
–
29
Momtaz et al.
2013
2010–2011
Isfahan and Shahrekord/Central
PCR
Food
Fresh fish/shrimp samples
Cross sectional
300
18
4b, 1/2b, 1/2a
2
24
30
Salehian et al.
2013
2012
Sari/North
Culture
Food
Traditional ice cream
Cross sectional
50
1
–
–
31
Zarei et al.
2013
UN
Ahvaz/ Southwest
PCR
Food
Beef, buffalo and lamb meats
Cross sectional
210
7
–
–
–
32
Akya et al.
2013
UN
Kermanshah/West
Culture
Food
Dairy, meat, products,RTE
Cross sectional
530
3
–
56
66
33
Shakib et al.
2013
UN
Lorestan/West
PCR
Human
Pregnant women
Cross sectional
100
0
–
–
–
34
Rahimi et al.
2014
2010–2011
Fars and Khuzestan/South and Southwest
PCR
Food
Bulk milk, camel, Water, buffalo, ovine, caprine,
Cross sectional
260
7
–
13
27
3
Eslami et al.
2014
2012–2013
Tehran/North
PCR
Human
Women with abortion
Cross sectional
96
16
–
–
–
35
Alidoosti et al.
2014
2012
Isfahan/Central
Culture
Animal
domestic dogs
92
1
1/2b
–
–
36
Jamali et al.
2014
2008–2010
Tehran/North
Culture
Animal
Duck, goose intestinal contents
Cross sectional
471
19
–
5
58
37
Moosavy et al.
2014
UN
Tabriz/Northwest
Culture
Food
Raw milk
Cross sectional
18
9
–
–
–
38
Haghi et al.
2015
2014
Zanjan/West
PCR
Food
Bovine milk, ovine milk
Cross sectional
60
0
–
–
–
39
Haghroosta et al.
2015
UN
Ahvaz/ Southwest
Serologic
Human
Pregnant women with spontaneous abortion and healthy pregnant women
Cross sectional
180
43
–
–
–
40
Jamali et al.
2015
2012–2014
Mazandaran/North
PCR
Food
Raw fish
Cross sectional
488
104
1/2a, 4b, 1/2b
34
37
41
Soltan Dallal et al.
2015
2013
Tehran/North
Culture
Food
Vegetables, salads
Cross sectional
200
1
–
–
48
42
Mashak et al.
2015
2011–2012
Tehran/North
Culture
Food
Fresh, frozen meats
Cross sectional
410
115
1/2a, 4b, 4c, 3b
–
–
43
Mansouri-Najand et al.
2015
2011
Kerman/Central
PCR
Food
Raw milk
Cross sectional
100
5
–
–
–
44
Pourkaveh et al.
2016
2015
Tehran/North
PCR
Human
Women with spontaneous abortion
Cross sectional
317
54
–
–
–
45
Abdollahzadeh et al.
2016
2014–2015
Karaj and Tehran/North
PCR
Food
Fish, shrimp, RTE seafood
Cross sectional
201
5
1/2b, 3b, 7, 1/2a, 3a
–
18
46
Pournajaf et al.
2016
2012–2015
Tehran/North
PCR
Human
Patients with spontaneous abortions
Cross sectional
170
14
–
–
–
47
Food
Dairy products/meat,
317
20
Animal
Domestic animals
130
12
Zeinali et al.
2017
2013
Mashhad/North east
PCR
Animal
Fresh chicken carcasses
Cross sectional
200
36
–
23
80
48
Lotfollahi et al.
2017
2013–2015
Tabriz/North west
PCR
Food
Sausage, milk, cheese, chicken, meat
Cross sectional
267
8
(1/2c or 3c), (4b, 4d or 4e), (1/2a or 3a)
–
–
49
Human
Pregnant woman with a abortion
125
11
Animal
Goat and sheep carcasses
50
3
Eleven studies investigated the prevalence of L. monocytogenes in humans. From those studies, the pooled prevalence of L. monocytogenes was 10% (95% CI: 7–12%) ranging from 0 to 28% (Fig. 2). There was a significant heterogeneity among the 11 studies (χ2 = 331.98; p < 0.001; I2 = 97.2%). The funnel plot for publication bias showed evidence of asymmetry. Additionally, Begg’s and Egger’s tests were performed to quantitatively evaluate the publication biases. According to the results of Begg’s test (z = 1.48, p = 0.02) and Egger’s test (t = 5.21, p < 0.001) a significant publication bias was observed.
×
According to the included publications, in nine studies the prevalence of L. monocytogenes was investigated in animals. The pooled prevalence of L. monocytogenes was estimated at 7% (95% CI: 4–10%) ranging from 1 to 18% (Fig. 3). There was a significant heterogeneity among the nine studies (χ2 = 85.46; p < 0.001; I2 = 90.64%). The symmetric funnel plot showed no evidence of publication bias and confirmed by the results of Begg’s test (z = 0.21, p = 0.835) and Egger’s test (t = 1.62, p = 0.116).
×
We found 32 articles which investigated the prevalence of L. monocytogenes in foods samples. The pooled prevalence of L. monocytogenes in Iranian food samples was estimated at 4% (95% CI: 3–5%) ranging from 0 to 50% (Fig. 4). Based on Q statistic and the I2 index heterogeneity was significant (χ2 = 573.757; p < 0.001; I2 = 94.97%). There was evidence of strong publication bias from the funnel plot of the included articles (Fig. 5); it was confirmed by Begg’s rank correlation analysis (z = 3.73, p < 0.001). However, Egger’s regression analysis showed a significant publication bias (t = 1.62, p = 0.116).
×
×
Of the totally included articles, only in 12 studies the distribution of L. monocytogenes serotypes was reported. From those studies, it was concluded that 4b, 1/2a, and 1/2b were the most prevalent serotype. Furthermore, the pooled prevalence of L. inocua was 5.6% ranging from 4.1 to 7.7%.
The results of subgroup analysis based on geographic location in human samples showed that pooled prevalence of L. monocytogenes was 14% (95% CI: 1–36%; n = 3 studies), 10% (95% CI: 4–18%; n = 7 studies), and 1% (95% CI: 0–5%; n = 1 studies) in South, North (West and East) and West of Iran, respectively (Additional file 2: Figure S1). The results of subgroup analysis based on diagnostic methods in human samples showed that pooled prevalence of L. monocytogenes was 1% (95% CI: 0–3%; n = 3 studies), 26% (95% CI: 23–30%; n = 2 studies), and 11% (95% CI: 4–17%; n = 6 studies) based on culture, serology and PCR methods, respectively (Additional file 2: Figure S1).
Anzeige
The results of subgroup analysis based on geographic location in food samples showed that pooled prevalence of L. monocytogenes was 7% (95% CI: 4–10%; n = 13 studies), 4% (95% CI: 2–5%; n = 11 studies), and 2% (95% CI: 1–3%; n = 4 studies), 3% (95% CI: 3–4%; n = 2 studies) in North (West and East), Central, South and all parts of Iran, respectively (Additional file 2: Figure S1).
The results of subgroup analysis based on the diagnostic methods in food samples showed that pooled prevalence of L. monocytogenes was 3% (95% CI: 1–4%; n = 11 studies), 5% (95% CI: 3–6%; n = 19 studies), and 12% (95% CI: 9–14%; n = 1 studies), 2% (95% CI: 1–4%; n = 1 studies) based on culture, PCR, Real-Time PCR and culture and serology methods, respectively (Additional file 2: Figure S1).
The results of subgroup analysis based on geographic location in Animal samples showed that pooled prevalence of L. monocytogenes was 9% (95% CI: 5–13%; n = 5 studies), 2% (95% CI: 0–4%; n = 2 studies), and 7% (95% CI: 3–14%; n = 1 studies) in North (West and East), Central and South of Iran, respectively (Additional file 2: Figure S1). The results of subgroup analysis based on the diagnostic methods in Animal samples showed that pooled prevalence of L. monocytogenes was 10% (95% CI: 6–13%; n = 5 studies) and 3% (95% CI: 1–5%; n = 3 studies) based on PCR and methods, respectively (Additional file 2: Figure S1).
Sensitivity analysis and meta-regression
The sample size of included studies could not be accounted as the causes of heterogeneity due to the result of carried meta-regression analysis in which no possible associated effect was observed between a sample size of included studies and pooled prevalence.
Anzeige
Besides, sensitivity analysis’s results concluded that none of the incorporated studies has the ability to change the overall prevalence substantially (Additional file 3: Figure S2).
Discussion
Direct transmission of L. monocytogenes from the infected animals or contaminated raw products is the main route of human cross-contamination [54]. The unique ability of this microorganism to survive food preservation or hostile environments and the presence of numerous bacterial surface components and extracellular virulence factors make L. monocytogenes as a serious threat to food safety [1, 55]. To the best of our knowledge, this study is the first comprehensive systematic review of the prevalence of L. monocytogenes in foods, animal and human origin from Iran, simultaneously. Based on the meta-analysis results, the overall estimate of L. monocytogenes prevalence among human origin with 10% was slightly higher than animal and food resources, i.e. 7% and 4%, respectively. However, some reasons may explain the higher prevalence of L. monocytogenes in Iranian population compared to environmental sources. First, most of the human origin studies were performed on susceptible groups including pregnant women or hospitalized patients, so the burden of L. monocytogenes infections would be expected to be lower in the general community. Second, in two studies with the highest isolation rate, authors used the serological method for detection of L. monocytogenes among the participants [14, 44] because antigenic cross-reactivity serological methods have lower discriminatory power in epidemiological studies compared to molecular methods [56].
Due to the multifactorial nature of L. monocytogenes prevalence, its international comparison is challenging. It seems that some factors have more profound effects on the prevalence of L. monocytogenes. Regarding the role of sample type, with some variation incidence of L. monocytogenes contamination in dairy products tends to be lower than other resources such as vegetables or meat products (mostly less than 10%) [57‐64]. Based on previous reports, the infection rate of domestic and wild animals is frequently higher than foods origin and has a much more variation [65‐71].
Gain a global estimate of L. monocytogenes infections in human is even more challenging since most of the studies looking in the distinct range of society or samples [72‐78]. Besides the variation according to the origin of isolation, various incidence rates of L. monocytogenes may arise from differences in the sample size, seasonal variability, and geographical distribution.
Anzeige
Listeria innocua is a ubiquitous non-pathogenic membrane of genus Listeria. This bacterium does not seem to carry the virulence-associated genes described in pathogenic species [79]. However, recently it has been shown that L. innocua can invade bovine trophoblasts, but it is unable to multiply in the intracellular environment [80]. In our findings the isolation rate of L. innocua among Iranian food resources was remarkable. To date, there is no report of human complication by this bacterium from Iran; however, two cases of L. innocua human infections were reported in European countries [79, 81]. These observations make us keep in mind that we should not rule out the potential risk of Listeria contamination rather than L. monocytogenes.
Analysis of the included studies revealed serotypes 4b, 1/2a, and 1/2b as the most prevalent serotypes. From annual trends of serotypes changes, it seems that 4b serotype is losing its dominant position and replaced by 1/2a and 1/2b. However, serotypes can be variable during different time periods, seasons or geographical distributions, and different sample type. Wang et al. showed 648 food samples collected within years 2013–2014 in Shanghai, China the majority of the isolates (more than 80%) belonged to serotypes 1/2a, and 1/2b [58]. Kevenk et al. from Turkey reported the presence of four different serotypes (1/2a, 1/2b, 1/2c, and 4b) in isolates obtained from milk and dairy products [61]. Haley et al. showed the predominance of 3 serogroups (1/2a, 1/2b, and 4b) in the isolates collected during 2004 and 2010 within a U.S. dairy herd [82]. In a study on several regions of Brazil from 1975 to 2013, with the same serotype distribution, Almeida and colleagues introduced 4b, 1/2b, and 1/2c, as the main serotypes in human and food sources [83]. Serotypes l/2a, l/2b, and 4b were the most prevalent serotypes in sows and fattening pigs in France in 2008 [70]. Hasegawa et al. showed the predominance of 1/2b, 1/2a, and 4b serotypes among black beef cattle in Japan [68]. Surveillance of invasive listeriosis within the years 2006–2010 in Italy, revealed serotypes 1/2a, 4b, and 1/2b as the frequent types [84]. When rank correlation methods show bias, the bias is likely evidence of small studies effect [85]. Meanwhile, meta-regression analysis showed that weight of studies could not be considered as a confounding factor. Also, sensitivity analysis on included studies indicated that exclusion of any study has no significant effect on the estimated pooled prevalence.
The limitations of our systematic review include the following: Firstly, due to the extent of L. monocytogenes has not yet been examined in many regions of Iran, we cannot fully represent the frequency of L. monocytogenes in the country. Secondly, the studies could not fully indicate the prevalence of L. inocua in Iran, because the prevalence of L. inocua has not yet been surveyed in many studies conducted in Iran. Third, heterogeneity was detected among the included studies therefore, the results should be interpreted with caution.
Conclusions
The results of the present study provide good epidemiological information about the contamination status and distribution of L. monocytogenes among Iranian resources. The prevalence of L. monocytogenes and prevalent serotypes in Iran is comparable with other parts of the world. Although the overall prevalence of human cross-contamination source was low, awareness about the source of contamination is very important because of a higher incidence of infections in susceptible groups.
Acknowledgments
We thanks from all member of the Clinical Research Development Center of Baqiyatallah hospital. This study was financially supported in part by “Clinical Research Development Center of Baqiyatallah hospital, Tehran, Iran.
Funding
We would like to thank from the “Clinical Research Development Center of Baqiyatallah hospital” for their kindly cooperation. This study was financially supported in part by “Clinical Research Development Center of Baqiyatallah hospital”.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Ethics approval and consent to participate
Not applicable.
Consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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