Background
Methods
Ethics statement
Placentas
Microarray hybridization
Quantitative real-time PCR analysis
LncRNA | Primer |
---|---|
GAPDH
| 5′GAGTGGGTGTCGCTGTTGA3′ |
ENST00000437593
| 5′ GACCCATTCAAACTCTTTCACC3′ |
BC017431
| 5′ AAGCTGTCTGGTGCTGCTCTG3′ |
BF328678
| 5′ GGTAGAAGCGGATGAGTAGAAATAC3′ |
ENST00000423797
| 5′ GCAAGGAGAAGTGCCCAGAT3′ |
BX483760
| 5′ CCAGGCTGGTTTCAAACTCC3′ |
AA451649
| 5′ CTGAAGTGGAAGTTACAAGGAGGT3′ |
DN918055
| 5′ CTCCGCCATATTTGCCGTAC3′ |
AX747492
| 5′ CCACCGATGTCTGCCTATGTC 3′ |
BG258490
| 5′ CCAGTCTAGCCAACATAGCAAAC3′ |
BF667001
| 5′ GTTGTGGGTCGGTGTTTCC3′ |
mRNA | Primers | Annealing (°C) | Amplicom (bp) |
---|---|---|---|
GAPDH
| F:5′GGGAAACTGTGGCGTGAT3′ | 60 | 299 |
R:5′GAGTGGGTGTCGCTGTTGA3′ | |||
ADAMTS15
| F:5′TGTGAAAGTCTGTGAGGAGGTGT3′ | 60 | 127 |
R:5′CAGGAAGTCGGTGATGATGG3′ | |||
AQPEP
| F:5′GGACACGGAATACATGGTGC3′ | 60 | 137 |
R:5′CCTGGTCGGTGTAGACGTTG3′ | |||
EML4
| F:5′TGAAGAGCCATGCAACGAGA3′ | 60 | 95 |
R:5′AGCCAGGGTGTTAGGACGAG3′ | |||
NBPF10
| F:5′ATCAGCTTCGCCCTTTACG3′ | 60 | 82 |
R:5′TGACTCCCATCTGGAACACC3′ | |||
PAM
| F:5′CCAGACCCGTAGTTCCTATTGA3′ | 60 | 96 |
R:5′ACATGCAGAAGTATGTATCGGACT3′ | |||
PDE4B
| F:5′CAGAAAGCAAGACCAGGAAGC3′ | 60 | 185 |
R:5′GGATAAGTTCCCGAAACTTAGTGC3′ | |||
PPP2R5C
| F:5′TTCATCGAGGTCTCGGGTAT3′ | 60 | 97 |
R:5′CTTGAAGTGACAGCAGTGGG3′ | |||
STAM
| F:5′TGGCAGTGACACCAAAGATAGAG3′ | 60 | 141 |
R:5′TAGCAAGGGATTAGACAGACGAA3′ | |||
TACC2
| F:5′GACACCTGTGATGAGTCCGTTG3′ | 60 | 149 |
R:5′TTCTTGGCGGGCTTGTTTAG3′ | |||
TATDN1
| F:5′TGAAAACTGAAGCTAATTTGGAA3′ | 60 | 211 |
R:5′ GCAGGGTTCATTTCTGTCTTT3′ |
Results
Identification of differentially expressed lncRNAs in comparisons of group AC (PTB & PPROM) vs. group BD (FTB & PROM) and group CD (PPROM & PROM) vs. group AB (PTB & FTB)
Metabolic pathways involved in group AC (PTB & PPROM) vs. group BD (FTB & PROM) and group CD (PPROM & PROM) vs. group AB (PTB & FTB)
Quantitative real-time PCR validation of differentially expressed lncRNAs and their associated mRNAs
PPROM vs. sPTB | PPROM vs. PROM | PPROM vs. FTB | sPTB vs. FTB | PROM vs. FTB | sPTB vs. PROM | [PPROM + sPTB] vs. [FTB + PROM] | [PPROM + PROM] vs. [sPTB + FTB] | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
lncRNA | mRNA | lncRNA | mRNA | lncRNA | mRNA | lncRNA | mRNA | lncRNA | mRNA | lncRNA | mRNA | lncRNA | mRNA | lncRNA | mRNA | lncRNA | mRNA |
BF328678
|
ADAMTS15
|
Down
|
Down
| ||||||||||||||
BG258490
|
PPP2R5C
|
Down
|
Down
|
Down
|
Down
|
Down
|
Down
| ||||||||||
AA451649
|
TATDN1
|
Down
|
Up
|
Up
|
Down
|
Down
|
Up
| ||||||||||
BF667001
|
STAM
|
Down
|
Up
|
Up
|
Down
|
Down
|
Up
|
Down
|
Up
|
Down
|
Up
| ||||||
ENST00000423797
|
EML4
|
Down
|
Up
| ||||||||||||||
AX747492
|
NBPF10
|
Up
|
Up
|
Down
|
Down
|
Up
|
Up
|
Up
|
Up
| ||||||||
BC107431
|
PAM
|
Up
|
Up
| ||||||||||||||
BX483760
|
AQPEP
|
Up
|
Up
|
Down
|
Up
|
Up
|
Up
| ||||||||||
DN918055
|
PDE4B
|
Up
|
Down
|
Down
|
Up
|
Up
|
Down
|
Up
|
Down
|
Up
|
Down
| ||||||
ENST00000437593
|
TACC2
|
Up
|
Down
|
Seqname | PPROM vs. sPTB | PPROM vs. PROM | PPROM vs. FTB | sPTB vs. FTB | PROM vs. FTB | sPTB vs. PROM | [PPROM + sPTB] vs. [FTB + PROM] | [PPROM + PROM] vs. [sPTB + FTB] | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
lncRNA | mRNA | lncRNA | mRNA | lncRNA | mRNA | lncRNA | mRNA | lncRNA | mRNA | lncRNA | mRNA | lncRNA | mRNA | lncRNA | mRNA | lncRNA | mRNA |
BF328678
|
ADAMTS15
|
down
|
up
|
up
|
up
|
up
|
up
|
down
|
up
|
down
|
down
|
down
|
Up
|
up
|
up
|
down
|
down
|
BG258490
|
PPP2R5C
|
down
|
down
|
up
|
up
|
up
|
down
|
down
|
down
|
down
|
down
|
down
|
Up
|
up
|
up
|
down
|
down
|
AA451649
|
TATDN1
|
down
|
down
|
up
|
up
|
down
|
up
|
down
|
up
|
down
|
down
|
down
|
Up
|
down
|
up
|
down
|
down
|
BF667001
|
STAM
|
down
|
up
|
up
|
down
|
up
|
down
|
down
|
up
|
down
|
up
|
up
|
Up
|
up
|
up
|
down
|
up
|
ENST00000423797
|
EML4
|
down
|
up
|
up
|
down
|
up
|
down
|
down
|
up
|
down
|
up
|
up
|
Up
|
up
|
up
|
down
|
up
|
AX747492
|
NBPF10
|
up
|
Up
|
down
|
down
|
down
|
down
|
up
|
up
|
up
|
up
|
up
|
Down
|
down
|
down
|
up
|
up
|
BC107431
|
PAM
|
down
|
Up
|
up
|
up
|
up
|
up
|
down
|
up
|
down
|
up
|
up
|
Up
|
up
|
up
|
down
|
up
|
BX483760
|
AQPEP
|
up
|
down
|
down
|
up
|
down
|
up
|
up
|
down
|
up
|
up
|
up
|
Down
|
down
|
down
|
up
|
down
|
DN918055
|
PDE4B
|
up
|
down
|
down
|
up
|
up
|
down
|
up
|
down
|
up
|
down
|
up
|
Up
|
up
|
down
|
up
|
down
|
ENST00000437593
|
TACC2
|
up
|
Up
|
down
|
up
|
up
|
down
|
up
|
up
|
up
|
down
|
up
|
Up
|
up
|
up
|
up
|
up
|
The target mRNAs of IncRNA interaction relationship analysis by GeneMANIA
Discussion
PPROM vs. PTB | Strand* (lncRNA/mRNA) | lncRNA | mRNA | Gene name | Possible regulation mechanism |
---|---|---|---|---|---|
lncRNA and mRNA with same expression pattern and located in the same strand
|
+/+
|
↑
|
↑
|
TACC2
|
LncRNAs have miRNA-binding sites and compete for the miRNAs of mRNA.
|
NBPF10
| |||||
↓
|
↓
|
PPP2R5C
| |||
lncRNA and mRNA with opposite expression patterns and located in the same strand
|
+/+
|
↓
|
↑
|
PAM
|
The product of gene is a bifunctional transcript, with one isoform as lncRNA and another as mRNA.
|
↑
|
↓
|
AQPEP
| |||
PDE4B
| |||||
lncRNA and mRNA with opposite expression patterns and located in opposite strands
|
−/+
|
↓
|
↑
|
ADAMTS15
|
LncRNA is the host to miRNAs which regulate mRNA through RNAi.
|
EML4
| |||||
STAM
| |||||
ncRNA and mRNA with same expression pattern and located in opposite strands
|
+/−
|
↓
|
↓
|
TATDN1
|
LncRNA increases mRNA stability through masking miRNA site of mRNA.
|