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01.12.2018 | Research article | Ausgabe 1/2018 Open Access

BMC Cancer 1/2018

Epithelial cell adhesion molecule (EpCAM) is involved in prostate cancer chemotherapy/radiotherapy response in vivo

Zeitschrift:
BMC Cancer > Ausgabe 1/2018
Autoren:
Jie Ni, Paul Cozzi, Julia Beretov, Wei Duan, Joseph Bucci, Peter Graham, Yong Li
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1186/​s12885-018-5010-5) contains supplementary material, which is available to authorized users.

Abstract

Background

Development of chemo−/radioresistance is a major challenge for the current prostate cancer (CaP) therapy. We have previously demonstrated that epithelial cell adhesion molecule (EpCAM) is associated with CaP growth and therapeutic resistance in vitro, however, the role of EpCAM in CaP in vivo is not fully elucidated. Here, we aimed to investigate how expression of EpCAM is involved in CaP growth and chemo−/radiotherapy response in NOD/SCID mouse models in vivo and to validate its role as a therapeutic target for CaP therapy.

Methods

EpCAM was knocked down in PC-3 CaP cell line using short hairpin RNA (shRNA). The effect of EpCAM-knockdown (KD) on tumour growth, chemo−/radiotherapy response and animal survival was evaluated on subcutaneous (s.c) and orthotopic mouse models.

Results

We found that KD of EpCAM significantly inhibited tumour growth, increased xenograft sensitivity to chemotherapy/radiotherapy, and prolonged the survival of tumour-bearing mice. In addition, we demonstrated that KD of EpCAM is associated with downregulation of the PI3K/Akt/mTOR pathway.

Conclusions

In conclusion, our data confirms that CaP growth and chemo−/radioresistance in vivo is associated with over-expression of EpCAM, which serves both a functional biomarker and promising therapeutic target.
Zusatzmaterial
Additional file 1: Table S1. List of antibodies used in Western Blot and immunohistochemistry. (DOCX 14 kb)
12885_2018_5010_MOESM1_ESM.docx
Additional file 2: Table S2. Survival information of the subcutaneous mouse CaP model. (DOCX 13 kb)
12885_2018_5010_MOESM2_ESM.docx
Additional file 3: Table S3. The staining intensity of various markers in subcutaneous xenografts CaP model. (DOCX 13 kb)
12885_2018_5010_MOESM3_ESM.docx
Literatur
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