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01.12.2017 | Primary Research | Ausgabe 1/2017 Open Access

Cancer Cell International 1/2017

Establishment and evaluation of four different types of patient-derived xenograft models

Zeitschrift:
Cancer Cell International > Ausgabe 1/2017
Autoren:
Xiaoqian Ji, Siyu Chen, Yanwu Guo, Wende Li, Xiaolong Qi, Han Yang, Sa Xiao, Guang Fang, Jinfang Hu, Chuangyu Wen, Huanliang Liu, Zhen Han, Guangxu Deng, Qingbin Yang, Xiangling Yang, Yuting Xu, Zhihong Peng, Fengping Li, Nvlue Cai, Guoxin Li, Ren Huang
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1186/​s12935-017-0497-4) contains supplementary material, which is available to authorized users.
Xiaoqian Ji and Siyu Chen contributed equally to this work

Abstract

Background

Patient-derived xenografts (PDX) have a biologically stable in tumor architecture, drug responsiveness, mutational status and global gene-expression patterns. Numerous PDX models have been established to date, however their thorough characterization regarding the tumor formation and rates of tumor growth in the established models remains a challenging task. Our study aimed to provide more detailed information for establishing the PDX models successfully and effectively.

Methods

We transplanted four different types of solid tumors from 108 Chinese patients, including 21 glioblastoma (GBM), 11 lung cancers (LC), 54 gastric cancers (GC) and 21 colorectal cancers (CRC), and took tumor tissues passaged for three successive generations. Here we report the rate of tumor formation, tumor-forming times, tumor growth curves and mortality of mice in PDX model. We also report H&E staining and immunohistochemistry for HLA-A, CD45, Ki67, GFAP, and CEA protein expression between patient cancer tissues and PDX models.

Results

Tumor formation rate increased significantly in subsequent tumor generations. Also, the survival rates of GC and CRC were remarkably higher than GBM and LC. As for the time required for the formation of tumors, which reflects the tumor growth rate, indicated that tumor growth rate always increased as the generation number increased. The tumor growth curves also illustrate this law. Similarly, the survival rate of PDX mice gradually improved with the increased generation number in GC and CRC. And generally, there was more proliferation (Ki67+) in the PDX models than in the patient tumors, which was in accordance with the results of tumor growth rate. The histological findings confirm similar histological architecture and degrees of differentiation between patient cancer tissues and PDX models with statistical analysis by GraphPad Prism 5.0.

Conclusion

We established four different types of PDX models successfully, and our results add to the current understanding of the establishment of PDX models and may contribute to the extension of application of different types of PDX models.
Zusatzmaterial
Additional file 1: Table S1. Clinical characteristics of GBM patients.
Additional file 2: Table S2. Clinical and pathological characteristics of LC patients.
Additional file 3: Table S3. Clinical characteristics of GC patients.
Additional file 4: Table S4. Clinical and pathological characteristics of CRC patients.
Literatur
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