Erschienen in:
01.05.2010 | Poster presentation
Evaluating gene expression in formalin-fixed, paraffin-embedded breast cancer tissues using DASL®
verfasst von:
T Burr, R Dixon, A Green, I Ellis, C Murray
Erschienen in:
Breast Cancer Research
|
Sonderheft 1/2010
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Excerpt
The study of gene expression in conventionally processed tissues is hampered by degradation of mRNA. Expensive, low-multiplex, quantitative PCR methods can be unreliable due to the limited template sizes. One way to overcome this problem is to use array-based methods. DASL® technology relies on random priming for production of cDNA, in concert with universal bead arrays to allow the detection and relative quantitation of expression of specific gene subsets. …