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Clinical Oral Investigations
Erschienen in: Clinical Oral Investigations 4/2019

30.08.2018 | Original Article

Evaluation of bone turnover after bisphosphonate withdrawal and its influence on implant osseointegration: an in vivo study in rats

verfasst von: Fausto Frizzera, Mario Henrique Arruda Verzola, Rafael Scaf de Molon, Guilherme Jose Pimentel Lopes de Oliveira, Gabriela Giro, Luis Carlos Spolidorio, Rosa Maria Rodrigues Pereira, Sotirios Tetradis, Joni Augusto Cirelli, Silvana Regina Peres Orrico

Erschienen in: Clinical Oral Investigations | Ausgabe 4/2019

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Abstract

Objectives

The aim of this study was to investigate bone turnover alterations after alendronate (ALD) withdrawal and its influence on dental implants osseointegration.

Materials and methods

Seventy female Wistar rats were randomly divided in 2 groups that received on day 0 either placebo (control group—CTL; n = 10) or 1 mg/kg sodium alendronate (ALD; n = 60) once a week for 4 months. At day 120, ALD treatment was suspended for 50 animals. Then, a titanium implant was placed in the left tibia of each rat that were randomly allocated in five subgroups of ten animals each, according to the period of evaluation: day 0 (INT-0), day 7 (INT-7), day 14 (INT-14), day 28 (INT-28), and day 45 (INT-45) after ALD withdrawal. CTL group and a group that received ALD until the end of the experimental period (non-interrupted group—non-INT; n = 10) underwent implant placement on day 120. Animals were euthanized 28 days after implant surgery. Bone mineral density (BMD) of femur and lumbar vertebrae were evaluated by DXA, biochemical markers of bone turnover were analyzed by ELISA, and bone histomorphometry was performed to measure bone-to-implant contact (BIC) and bone area fraction occupancy (BAFO).

Results

All groups receiving ALD showed higher BMD values when compared to CTL group, which were maintained after its withdrawal. Decreased concentrations in all bone turnover markers were observed in the non-INT group, and in the groups in which ALD was discontinued compared to the CTL group. The non-INT group showed lower %BIC and notably changes in bone quality, which was persistent after drug withdrawal.

Conclusion

Collectively, the findings of this study demonstrated that ALD therapy decreased bone turnover and impaired bone quality and quantity around dental implants, and that its discontinuation did not reverse these findings.

Clinical relevance

The severe suppression of bone turnover caused by the prolonged use of ALD may alter the capacity of bone tissue to integrate with the implant threads impairing the osseointegration process.
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Metadaten
Titel
Evaluation of bone turnover after bisphosphonate withdrawal and its influence on implant osseointegration: an in vivo study in rats
verfasst von
Fausto Frizzera
Mario Henrique Arruda Verzola
Rafael Scaf de Molon
Guilherme Jose Pimentel Lopes de Oliveira
Gabriela Giro
Luis Carlos Spolidorio
Rosa Maria Rodrigues Pereira
Sotirios Tetradis
Joni Augusto Cirelli
Silvana Regina Peres Orrico
Publikationsdatum
30.08.2018
Verlag
Springer Berlin Heidelberg
Erschienen in
Clinical Oral Investigations / Ausgabe 4/2019
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-018-2612-x

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