Background
Methods
Chemicals
Collection of plant materials
Preparation of extraction
Test animals
Acute toxicity test
Phytochemical screening
Pharmacological tests
Open field test
Hole cross test
Forced swimming test
Tail suspension test
Thiopental sodium-induced sleeping time test
Statistical analysis
Results
Phytochemical screening
Plant constituents | Inference |
---|---|
Alkaloids | + |
Flavonoids | + |
Saponins | + |
Tannins | + |
Cardiac glycosides | + |
Carbohydrates | – |
Reducing sugars | – |
Proteins | – |
Glucosides | – |
Terpenoids | + |
Steroids | + |
Acute toxicity
Open field test
Treatment | Dose (mg/kg) | Number of square crossed | ||||
---|---|---|---|---|---|---|
0 min | 30 min | 60 min | 90 min | 120 min | ||
Control | 0.1 mL/mouse | 76.00 ± 1.64 | 72.20 ± 3.48 | 61.00 ± 2.66 | 41.40 ± 1.07 | 32.60 ± 1.36 |
Diazepam | 1 | 71.80 ± 3.26 | 55.80 ± 2.69* | 30.40 ± 2.04* | 15.00 ± 0.70* | 6.80 ± 0.58* |
MECD | 50 | 59.80 ± 1.96 | 49.00 ± 4.19* | 44.00 ± 1.22* | 27.60 ± 1.12* | 15.40 ± 0.74* |
MECD | 100 | 56.40 ± 2.42 | 43.20 ± 1.28* | 40.40 ± 2.06* | 19.60 ± 0.92* | 9.80 ± 0.66* |
MECD | 200 | 63.60 ± 1.93 | 51.00 ± 1.76* | 33.60 ± 1.83* | 17.40 ± 0.92* | 8.20 ± 0.58* |
Hole cross test
Treatment | Dose (mg/kg) | Number of hole crossed | ||||
---|---|---|---|---|---|---|
0 min | 30 min | 60 min | 90 min | 120 min | ||
Control | 0.1 mL/mouse | 16.40 ± 0.92 | 12.40 ± 1.12 | 9.40 ± 0.51 | 6.60 ± 0.81 | 4.00 ± 0.70 |
Diazepam | 1 | 14.00 ± 1.41 | 5.40 ± 0.51* | 3.40 ± 0.24* | 1.20 ± 0.37* | 0.60 ± 0.24* |
MECD | 50 | 14.40 ± 1.69 | 7.60 ± 0.40* | 6.20 ± 1.02* | 3.00 ± 0.54* | 2.40 ± 0.51 |
MECD | 100 | 13.40 ± 0.92 | 6.60 ± 0.74* | 5.00 ± 0.89* | 2.40 ± 0.67* | 1.60 ± 0.51* |
MECD | 200 | 15.00 ± 0.83 | 6.40 ± 0.51* | 4.20 ± 0.37* | 1.80 ± 0.37* | 1.00 ± 0.31* |
Forced swimming test
Treatment | Dose (mg/kg) | Immobility time (s) |
---|---|---|
Control | 0.1 mL/mouse | 62.00 ± 2.92 |
Diazepam | 1 | 177.00 ± 2.55* |
MECD | 50 | 73.20 ± 1.99 |
MECD | 100 | 87.20 ± 3.61* |
MECD | 200 | 141.40 ± 4.25* |
Tail suspension test
Treatment | Dose (mg/kg) | Immobility time(s) |
---|---|---|
Control | 0.1 mL/mouse | 84.80 ± 2.41 |
Diazepam | 1 | 219.80 ± 3.98* |
MECD | 50 | 106.00 ± 2.12* |
MECD | 100 | 130.80 ± 2.22* |
MECD | 200 | 191.20 ± 2.35* |
Thiopental sodium-induced sleeping time test
Treatment | Dose (mg/kg) | Onset of action (min) | Duration of sleeping time (min) |
---|---|---|---|
Control | 0.1 mL/mouse | 4.95 ± 0.41 | 27.00 ± 1.92 |
Diazepam | 1 | 3.01 ± 0.13* | 91.20 ± 1.93* |
MECD | 50 | 4.78 ± 0.22 | 38.80 ± 1.36* |
MECD | 100 | 4.56 ± 0.22 | 46.80 ± 1.28* |
MECD | 200 | 3.74 ± 0.24* | 76.20 ± 2.15* |