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05.05.2024 | Original Article

Evaluation of early bacterial adhesion on CAD/CAM dental materials: an in situ study

verfasst von: Stefan Vulović, Aleksandar Todorović, Boško Toljić, Nataša Nikolić-Jakoba, Tamara Vlajić Tovilović, Aleksandra Milić-Lemić

Erschienen in: Odontology | Ausgabe 1/2025

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Abstract

The aim of this research was to determine if there are differences in early bacterial adhesion among CAD/CAM dental materials after 24 h exposure in the oral environment. One hundred twenty specimens were prepared according to the manufacturer’s recommendations and divided into six groups: RBC (resin-based composite), PMMA (polymethyl methacrylate), PEEK (polyether ether ketone), ZP (zirconia polished), ZG (zirconia glazed), and cobalt-chromium alloy (CoCr alloy). Twenty healthy participants were instructed to carry an intraoral device with six specimens, one per group, for 24 h. Thereafter, real-time polymerase chain reaction (qPCR) and scanning electron microscopy (SEM) analyses enabled quantification and 2D view of biofilm formed on the specimens’ surfaces. Kruskal–Wallis test and Dunn’s post hoc analysis were used for inter-group comparison and data were presented as median (minimum–maximum). RBC specimens accumulated less bacteria, in comparison with ZG (p = 0.017) and PEEK specimens (p = 0.030), that dominated with the highest amount of adhered bacterial biofilm. PMMA, CoCr, and ZP specimens adhered more bacteria than RBC (p > 0.05), and less than ZG (p > 0.05) and PEEK (p > 0.05). The bacterial number varied considerably among participants. The obtained results enable a closer view into the susceptibility of CAD/CAM materials to microorganisms during the presence in the oral environment, which can be beneficial for a proper selection of these materials for a variety of dental restorations.

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Titel: Evaluation of early bacterial adhesion on CAD/CAM dental materials: an in situ study
verfasst von: Stefan Vulović
Aleksandar Todorović
Boško Toljić
Nataša Nikolić-Jakoba
Tamara Vlajić Tovilović
Aleksandra Milić-Lemić
Publikationsdatum: 05.05.2024
Verlag: Springer Nature Singapore
Erschienen in: Odontology / Ausgabe 1/2025
Print ISSN: 1618-1247
Elektronische ISSN: 1618-1255
DOI: https://doi.org/10.1007/s10266-024-00944-y

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Evaluation of early bacterial adhesion on CAD/CAM dental materials: an in situ study

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