Introduction
Materials and methods
Study design
Patient population
Case | Sex | Age | Diagnosis | Localization | WHO Grade | Serum MMPs (vol × 10−3) | Tissue MMP-9 | ||||
---|---|---|---|---|---|---|---|---|---|---|---|
MMP-9 (240 kDa) | MMP-9 (130 kDa) | MMP-9 (92 kDa) | MMP-2 (72 kDa) | Neoplastic vessels | Neoplastic glial cells | ||||||
1 | M | 43 | A | Cerebellum | I | nd | nd | 243 | 574 | 0 | 0 |
2 | F | 17 | A | Encephalon | I | 207 | 415 | 1482 | 249 | 0 | 0 |
3 | M | 39 | G | Cerebellum | I | 106 | 50 | 1243 | 576 | 0 | 0 |
4 | F | 32 | O | Left temporal lobe | II | 248 | 113 | 1765 | 942 | 0 | +++ |
5 | M | 40 | O | Left frontal lobe | II | 62 | 58 | 1051 | 547 | 0 | +++ |
6 | M | 49 | AO | Right frontal lobe | III | 103 | 123 | 1262 | 410 | 0 | +++ |
7 | F | 82 | AO | Right temporal lobe | III | 342 | 214 | 1684 | 893 | 0 | ++ |
8 | F | 73 | AO | Left frontal lobe | III | 185 | 102 | 1262 | 359 | 0 | ++ |
9 | M | 73 | AO | Encephalon | III | 110 | 61 | 708 | 310 | 0 | ++ |
10 | M | 68 | GMB | Left temporal lobe | IV | 88 | 38 | 1015 | 460 | ++ | 0 |
11 | M | 56 | GMB | Left temporal lobe | IV | 111 | 115 | 1394 | 493 | ++ | ++ |
12 | M | 48 | GMB | Left frontal lobe | IV | 47 | nd | 667 | 464 | +++ | + |
13 | M | 62 | GMB | Right temporal lobe | IV | 140 | nd | 1190 | 566 | ++ | + |
14 | F | 76 | GMB | Right parietal lobe | IV | 82 | 61 | 892 | 540 | ++ | ++ |
15 | M | 77 | GMB | Encephalon | IV | 117 | nd | 1096 | 392 | Not done | Not done |
16 | M | 75 | GMB | Right parietal lobe | IV | 194 | 96 | 1408 | 205 | Not done | Not done |
17 | M | 58 | GMB | Left temporal lobe | IV | 203 | nd | 1361 | 1026 | 0 | + |
18 | F | 63 | GMB | Right temporal lobe | IV | 101 | nd | 1130 | 526 | ++ | ++ |
19 | M | 78 | GMB | Right temporal lobe | IV | 162 | 61 | 470 | 70 | 0 | + |
20 | F | 45 | GMB | Left frontal lobe | IV | 77 | nd | 1117 | 308 | Not done | Not done |
Case | Sex | Age | Diagnosis | Localization | WHO Grade | Serum MMPs (vol × 10−3) | Ki-67 (%) (LI) | PR (%) (LI) | Tissue MMP-9 | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|
MMP-9 (240 kDa) | MMP-9 (130 kDa) | MMP-9 (92 kDa) | MMP-2 (72 kDa) | |||||||||
21 | M | 65 | Syncytial | Planum etmoidale | I | 62 | nd | 437 | 575 | 2 | 90 | 0 |
22 | F | 50 | Secretory | Left parietal lobe | I | 160 | 149 | 1318 | 196 | 6 | <10 | 0 |
23 | F | 70 | Microcystic | Encephalon | I | 93 | 60 | 1032 | 399 | 2 | <10 | 0 |
24 | F | 54 | Psammomatous | Spinal (D8–D9) | I | 58 | 111 | 1185 | 227 | 3 | 50 | 0 |
25 | F | 71 | Psammomatous | Left tentorium | I | 199 | 107 | 1008 | 290 | <1 | <10 | 0 |
26 | M | 36 | Psammomatous | Encephalon | I | 205 | 145 | 1494 | 1205 | 1–2 | 80 | + |
27 | F | 45 | Fibroblastic | Left ventricular trine | I | 90 | 52 | 1046 | 916 | 3 | 80 | +++ |
28 | F | 75 | Transitional | Planum etmoidale | I | 233 | 222 | 1217 | 470 | <3 | 70 | 0 |
29 | F | 68 | Transitional | Left parietal lobe | I | 56 | nd | 665 | 399 | 2 | <10 | +++ |
30 | F | 59 | Transitional | Cerebellum | I | 183 | 247 | 1425 | 1083 | 3 | 10 | ++ |
31 | F | 54 | Transitional | Right anterior cranial fossa | I | 77 | 130 | 1000 | 565 | 2 | 30 | + |
32 | F | 47 | Transitional | Right spheno-orbital | I | 74 | nd | 525 | 587 | 4 | <1 | + |
33 | F | 38 | Transitional | Planum etmoidale | I | 104 | 78 | 1336 | 318 | 2–3 | 90 | + |
34 | F | 49 | Transitional | Sphenoid | I | 49 | nd | 769 | 396 | 2 | 70 | + |
35 | M | 66 | Transitional | Parasagittal | I | 131 | 67 | 1092 | 643 | <1 | <1 | 0 |
36 | F | 36 | Transitional | Planum etmoidale | I | 44 | nd | 750 | 202 | <1 | <1 | + |
37 | M | 76 | Atypical | Right parietal convexity | II | 190 | nd | 1099 | 618 | 5 | 80 | + |
38 | F | 72 | Atypical | Dorsal (D8-D9) | II | 223 | 109 | 1362 | 546 | 13 | <10 | +++ |
39 | M | 70 | Atypical | Right frontal convexity | II | 157 | 138 | 1563 | 754 | 6 | 50 | 0 |
40 | F | 58 | Atypical | Left frontal convexity | II | 33 | nd | 572 | 569 | 7 | 50 | +++ |
41 | M | 84 | Atypical | Left parietal convexity | II | 148 | 106 | 1298 | 712 | 7–8 | <10 | ++ |
42 | M | 64 | Atypical | Parasagittal | II | 58 | nd | 803 | 461 | 10 | <10 | +++ |
43 | M | 77 | Atypical | Parasagittal | II | 73 | 74 | 1248 | 753 | 10–12 | <10 | + |
44 | M | 80 | Atypical | Left frontal convexity | II | 72 | 46 | 551 | 381 | 6–7 | 30 | +++ |
45 | F | 50 | Atypical | Right petrous | II | 114 | 124 | 1238 | 324 | 10 | <10 | +++ |
46 | F | 82 | Atypical | Right anterior cranial fossa | II | 156 | 54 | 1186 | 794 | 10 | 75 | + |
47 | F | 73 | Atypical | Left parietal lobe | II | 85 | nd | 937 | 511 | 6–7 | 50 | 0 |
48 | M | 51 | Atypical | Parasagittal | II | nd | nd | 756 | 48 | 50 | 40 | + |
Case | Sex | Age | Diagnosis | Localization | Serum MMPs (vol × 10−3) | |||
---|---|---|---|---|---|---|---|---|
MMP-9 (240 kDa) | MMP-9 (130 kDa) | MMP-9 (92 kDa) | MMP-2 (72 kDa) | |||||
49 | M | 64 | Melanoma | Left temporal lobe | 212 | nd | 1233 | 755 |
50 | M | 61 | Melanoma | Right frontal lobe | 160 | 67 | 1426 | 703 |
51 | M | 66 | Melanoma | Cerebellum | 208 | 104 | 1717 | 330 |
52 | M | 50 | Melanoma | Frontal lobe | 100 | 45 | 940 | 384 |
53 | F | 58 | Melanoma | Encephalon, NOS | 198 | 318 | 1183 | 540 |
54 | F | 52 | Melanoma | Left frontal lobe | 145 | 111 | 1450 | 1228 |
55 | M | 32 | Melanoma | Parietal lobe | 149 | 233 | 1194 | 334 |
56 | M | 77 | Melanoma | Encephalon, NOS | 306 | 719 | 2040 | 1132 |
57 | M | 67 | Melanoma | Right temporal lobe | 305 | 217 | 1531 | 470 |
58 | M | 80 | Melanoma | Right occipital lobe | 89 | nd | 556 | 353 |
59 | F | 87 | NSCLC | Right cerebellum | 254 | nd | 1202 | 518 |
60 | M | 57 | NSCLC | Left occipital lobe | 361 | 195 | 1672 | 485 |
61 | M | 48 | NSCLC | Encephalon, NOS | 185 | 72 | 1473 | 781 |
62 | M | 72 | NSCLC | Left frontal lobe | 140 | nd | 1277 | 535 |
63 | M | 55 | NSCLC | Left cerebellum | 403 | 235 | 1740 | 1012 |
64 | M | 71 | SCLC | Cerebellum | 122 | 147 | 1217 | 237 |
65 | F | 67 | Breast Ca | -- | 80 | nd | 1097 | 634 |
66 | F | 77 | Colon Ca | Cerebellum | 121 | 51 | 1066 | 334 |
67 | M | 73 | Kidney Ca | Left frontal lobe | 117 | 103 | 1077 | 213 |
68 | F | 77 | Urogenital Ca | Left frontal lobe | 135 | 164 | 1143 | 125 |
Serum samples
Materials
Gelatin zymography
Immunohistochemistry
Statistical analysis
Results
MMP-9 (240 kDa) | MMP-9 (130 kDa) | MMP-9 (92 kDa) | MMP-2 (72 kDa) | |
---|---|---|---|---|
Healthy controls | 51.7 [27; 112.5] | 61.4 [48.8; 90.4] | 762.6 [248.7; 1274.8] | 438.9 [201.9; 1117.6] |
High grade gliomas | 111.2 [47; 342.5]* | 98.8 [38.4; 213.8] | 1129.9 [470.2; 1684.2]* | 459.6 [69.8; 1025.7] |
Low grade meningiomas | 91.5 [48.6; 232.9]* | 120.2 [51.6; 247.2] | 1038.9 [436.6; 1493.9]* | 490.3 [195.8; 1205.4] |
High grade meningiomas | 113.7 [32.8; 222.6]* | 106 [45.6; 137.7] | 1185.9 [550.8; 1562.6]* | 617.8 [324.4; 794.4] |
Intracranial metastasis | 154.7 [79.6; 403.1]* | 146.8 [45.2; 719.1] | 1224.9 [556.2; 2039.7]* | 501.9 [125.1; 1228.4] |