nach oben

Lasers in Medical Science

Erschienen in:

19.07.2017 | Original Article

Evaluation of the effects of photobiomodulation on vertebras in two rat models of experimental osteoporosis

verfasst von: Mohammadjavad Fredoni, Mahdi Ghatrehsamani, Mohammad-amin Abdollahifar, Sahar Bayat, Mohammad Bayat

Erschienen in: Lasers in Medical Science | Ausgabe 7/2017

Einloggen, um Zugang zu erhalten

Abstract

The aim of this study was to evaluate the effects of photobiomodulation (PBM) on cancellous bone in rat models of ovariectomized induced osteoporosis (OVX-D) and glucocorticoid-induced osteoporosis (GIOP). The experiment comprised of nine groups. A group of healthy rats was used for baseline evaluations. The OVX-D rats were further divided into groups as follows: control rats with osteoporosis, OVX-D rats that received alendronate (1 mg/kg 60 days), OVX-D rats treated with pulsed wave laser (890 nm, 80 Hz, 900 s, 0.0061 W/cm², 5.5 J/cm², three times a week, 60 days), and OVX-D rats treated with alendronate + pulsed laser. Dexamethasone was administered to the remaining rats that were split into four groups: control, alendronate-treated rats, laser-treated rats, and GIOP rats treated with alendronate + laser. T12, L1, L2, and L3 vertebrae were subjected to laser. Results of the current study demonstrated that OVX-D and GIOP significantly decreased some stereological parameters, and type 1 collagen gene expression compared to the healthy group. There was a significant increase in osteoclast number in both OVX-D and glucocorticoid administration compared to the healthy group. However, the detrimental effect of the OVX-D procedure on bone was more serious than glucocorticoid administration. Results showed that laser alone had a detrimental effect on trabecular bone volume, and cortical bone volume in groups GIOP and OVX-D compared to those in the healthy group. Alendronate significantly improved total vertebral bone volume, trabecular bone volume, and cortical bone volume, in GIOP and OVX-D groups compared to the laser-treated groups. Furthermore, the alendronate + laser in OVX-D rats and GIOP rats produced significantly increased osteoblast number and type 1 collagen gene expression and caused a significant decrease in osteoclast number compared to the controls.

Bidwell JP, Alvarez MB, Hood M Jr, Childress P (2013) Functional impairment of bone formation in the pathogenesis of osteoporosis: the bone marrow regenerative competence. Curr Osteoporos Rep 1:117–125. doi:10.1007/s11914-013-0139-2 CrossRef

Briot K, Roux C (2015) Glucocorticoid-induced osteoporosis. RMD Open 1:e000014. doi:10.1136/rmdopen-2014-000014 CrossRefPubMedPubMedCentral

Bouillon R, Burckhardt P, Christiansen C, Fleisch H, Fujita T, Gennari C et al (1991) Consensus development conference: prophylaxis and treatment of osteoporosis. Am J Med 90:107–110CrossRef

Wright NC, Looker AC, Saag KG, Curtis JR, Delzell ES, Randall S et al (2014) The recent prevalence of osteoporosis and low bone mass in the United States based on bone mineral density at the femoral neck or lumbar spine. J Bone Miner Res 29:2520–2526. doi:10.1002/jbmr.2269 CrossRefPubMedPubMedCentral

Mansjur KQ, Kuroda S, Izawa T, Maeda Y, Sato M, Watanabe K, et al (2016) The effectiveness of human parathyroid hormone and low-intensity pulsed ultrasound on the fracture healing in osteoporotic bones. Ann Biomed Eng 1–9. doi:10.1007/s10439-015-1533-y

Izzah Ibrahim N, Mohamad S, Mohamed N, Nazrun SA (2013) Experimental fracture protocols in assessments of potential agents for osteoporotic fracture healing using rodent models. Curr Drug Targets 14:1642–1650. doi:10.2174/1389450114666131216224003 CrossRef

McNamara L (2010) Perspective on post-menopausal osteoporosis: establishing an interdisciplinary understanding of the sequence of events from the molecular level to whole bone fractures. J R Soc Interface 7:353–372. doi:10.1098/rsif.2009.0282 CrossRefPubMed

Khosla S, Westendorf JJ, Oursler MJ (2008) Building bone to reverse osteoporosis and repair fractures. J Clin Invest 118:421–428. doi:10.1172/JCI33612 CrossRefPubMedPubMedCentral

Cashman KD (2007) Diet, nutrition, and bone health. J Nutr 137:2507S–2512SPubMed

10.

Tee S-I, Yosipovitch G, Chan YC, Chua SH, Koh ET, Chan YH, Tan SS, Tsou IY, Tan SH (2012) Prevention of glucocorticoid-induced osteoporosis in immunobullous diseases with alendronate: a randomized, double-blind, placebo-controlled study. Arch Dermatol 148:307–314. doi:10.1001/archdermatol.2011.354 CrossRefPubMed

11.

da Silva JP, da Silva MA, Almeida APF, Junior IL, Matos AP (2010) Laser therapy in the tissue repair process: a literature review. Photomed Laser Surg 28:17–21. doi:10.1089/pho.2008.2372 CrossRefPubMed

12.

Ueda Y, Shimizu N (2003) Effects of pulse frequency of low-level laser therapy (LLLT) on bone nodule formation in rat calvarial cells. J Clin Laser Med Surg 21:271–277. doi:10.1089/104454703322564479 CrossRefPubMed

13.

Ueda Y, Shimizu N (2001) Pulse irradiation of low-power laser stimulates bone nodule formation. J Oral Sci 43:55–60. doi:10.2334/josnusd.43.55 CrossRefPubMed

14.

Sohn H, Ko Y, Park M, Kim D, Moon YL, Jeong YJ, Lee H, Moon Y, Jeong BC, Kim O, Lim W (2015) Effects of light-emitting diode irradiation on RANKL-induced osteoclastogenesis. Lasers Surg Med 47:745–755. doi:10.1002/lsm.22413 CrossRefPubMed

15.

Kiyosaki T, Mitsui N, Suzuki N, Shimizu N (2010) Low-level laser therapy stimulates mineralization via increased Runx2 expression and ERK phosphorylation in osteoblasts. Photomed Laser Surg 28(Suppl 1):S167–S172. doi:10.1089/pho.2009.2693 PubMed

16.

Xu M, Deng T, Mo F, Deng B, Lam W, Deng P, Zhang X, Liu S (2009) Low-intensity pulsed laser irradiation affects RANKL and OPG mRNA expression in rat calvarial cells. Photomed Laser Surg 27:309–315. doi:10.1089/pho.2008.2283 CrossRefPubMed

17.

Bayat M, Abdi S, Javadieh F, Mohsenifar Z, Rashid MR (2009) The effects of low-level laser therapy on bone in diabetic and nondiabetic rats. Photomed Laser Surg 27:703–708. doi:10.1089/pho.2008.2351 CrossRefPubMed

18.

Freidouni M, Nejati H, Salimi M, Bayat M, Amini A, Noruzian M, Asgharie MA, Rezaian M (2015) Evaluating glucocorticoid administration on biomechanical properties of rats’ tibial diaphysis. Iran Red Crescent Med J 17(3):e19389. doi:10.5812/ircmj.19389 eCollection 2015CrossRefPubMedPubMedCentral

19.

Najar A, Fridoni M, Rezaei F, Bayat S, Bayat M (2015) Supraphysiologic glucocorticoid administration increased biomechanical bone strength of rats’ vertebral body. Lab Anim Res 31:180–187. doi:10.5625/lar.2015.31.4.180 CrossRefPubMedPubMedCentral

20.

Cressoni MD, Giusti HH, Piao AC, de Paiva Carvalho RL, Anaruma CA, Casarotto RA (2010) Effect of GaAlAs laser irradiation on the epiphyseal cartilage of rats. Photomed Laser Surg 28:527–532. doi:10.1089/pho.2009.2572 CrossRefPubMed

21.

Bayat M, Fridoni M, Nejati H, Mostafavinia A, Salimi M, Ghatrehsamani M et al (2016) An evaluation of the effect of pulsed wave low-level laser therapy on the biomechanical properties of the vertebral body in two experimental osteoporosis rat models. Lasers Med Sci 31:305-14. doi:10.1007/s10103-015-1842-2 CrossRefPubMed

22.

Ko CY, Kang H, Ryu Y, Jung B, Kim H, Jeong D, Shin HI, Lim D, Kim HS (2013) The effects of minimally invasive laser needle system on suppression of trabecular bone loss induced by skeletal unloading. Lasers Med Sci 28:1495–1502. doi:10.1007/s10103-013- CrossRefPubMed

23.

Kang H, Ko CY, Ryu Y, Seo DH, Kim HS, Jung B (2012) Development of a minimally invasive laser needle system: effects on cortical bone of osteoporotic mice. Lasers Med Sci 27:965–969. doi:10.1007/s10103-011-1014-y CrossRefPubMed

24.

Chen Y, Cao Z, Zhang L, Xu X, Chen Y, Chen Y (2011) Low level laser can be a novel adjuvant method for orthodontic tooth movement on postmenopausal women. Med Hypotheses 76:479–481. doi:10.1016/j.mehy.2010.11.025 CrossRefPubMed

25.

Mohsenifar Z, Fridoni M, Ghatrehsamani M, Abdollahifar MA, Abbaszadeh H, Mostafavinia A, Fallahnezhad S, Asghari M, Bayat S, Bayat M (2016) Evaluation of the effects of pulsed wave LLLT on tibial diaphysis in two rat models of experimental osteoporosis, as examined by stereological and real-time PCR gene expression analyses. Lasers Med Sci 31:721–732. doi:10.1007/s10103-016-1916-9 CrossRefPubMed

26.

Fridoni M, Masteri Farahani R, Nejati H, Salimi M, Gharavi SM, Bayat M, Amini A, Torkman G, Bayat S (2015) Evaluation of the effects of LLLT on biomechanical properties of tibial diaphysis in two rat models of experimental osteoporosis by a three point bending test. Lasers Med Sci 30:1117–1125. doi:10.1007/s10103-014-1706-1 CrossRefPubMed

27.

Medalha CC, Amorim BO, Ferreira JM, Oliveira P, Pereira RM, Tim C, Lirani-Galvão AP, da Silva OL, Renno AC (2010) Comparison of the effects of electrical field stimulation and low-level laser therapy on bone loss in spinal cord-injured rats. Photomed Laser Surg 28:669–674. doi:10.1089/pho.2009.2691 CrossRefPubMed

28.

Diniz JS, Nicolau RA, de Melo ON, do Carmo Magalhães F, de Oliveira Pereira RD, Serakides R (2009) Effect of low-power gallium-aluminum-arsenium laser therapy (830 nm) in combination with bisphosphonate treatment on osteopenic bone structure: an experimental animal study. Lasers Med Sci 24:347–352. doi:10.1007/s10103-008-0568-9 CrossRefPubMed

29.

Renno AC, de Moura FM, dos Santos NS, Tirico RP, Bossini PS, Parizotto NA (2006) Effects of 830-nm laser light on preventing bone loss after ovariectomy. Photomed Laser Surg 24:642–645. doi:10.1089/pho.2006.24.642 CrossRefPubMed

30.

Muniz Renno AC, de Moura FM, dos Santos NS, Tirico RP, Bossini PS, Parizotto NA (2006) The effects of infrared-830 nm laser on exercised osteopenic rats. Lasers Med Sci 21:202–207. doi:10.1007/s10103-006-0396-8 CrossRefPubMed

31.

Kanis J, McCloskey E, Johansson H, Cooper C, Rizzoli R, Reginster J-Y (2013) European guidance for the diagnosis and management of osteoporosis in postmenopausal women. Osteoporos Int 24:23–57. doi:10.1007/s00198-012-2074-y CrossRefPubMed

32.

Hashmi JT, Huang YY, Sharma SK, Kurup DB, De Taboada L, Carroll JD, Hamblin MR (2010) Effect of pulsing in low-level light therapy. Lasers Surg Med 42:450–466. doi:10.1002/lsm.20950 CrossRefPubMedPubMedCentral

33.

Je A, Nb M, Sagreiya K (1987) The microanatomy of trabecular bone loss in normal aging men and women. Clin Orthop Relat Res 215:260–271 3802645

34.

Nordin B, Speed R, Aaron J, Crilly R (1981) Bone formation and resorption as the determinants of trabecular bone volume in postmenopausal osteoporosis. Lancet 318:277–279 6114324CrossRef

35.

Van Brussel M, Bultink I, Lems W (2009) Prevention of glucocorticoid-induced osteoporosis. Expert Opin Drug Deliv 10:997–1005. doi:10.1517/14656560902868225

36.

Yeh J, Chen M-M, Aloia J (1996) Ovariectomy-induced high turnover in cortical bone is dependent on pituitary hormone in rats. Bone 18:443–450CrossRefPubMed

37.

Jagtap VR, Ganu JV, Nagane NS (2011) BMD and serum intact osteocalcin in postmenopausal osteoporosis women. Indian J Clin Biochem 26:70–73CrossRefPubMed

38.

Green D, Wallace H (2003) Late effects of childhood cancer. CRC Press

39.

Parfitt AM (1987) Trabecular bone architecture in the pathogenesis and prevention of fracture. Am J Med 82:68–72CrossRefPubMed

40.

Lane NE, Yao W, Balooch M, Nalla RK, Balooch G, Habelitz S et al (2006) Glucocorticoid-treated mice have localized changes in trabecular bone material properties and osteocyte lacunar size that are not observed in placebo-treated or estrogen-deficient mice. J Bone Miner Res 21:466–476. doi:10.1359/JBMR.051103 CrossRefPubMed

41.

Russ JC, Dehoff RT (2012) Practical stereology. Springer Science & Business Media

42.

Lane NE, Thompson JM, Haupt D, Kimmel DB, Modin G, Kinney JH (1998) Acute changes in trabecular bone connectivity and osteoclast activity in the ovariectomized rat in vivo. J Bone Miner Res 13:229–236. doi:10.1359/jbmr CrossRefPubMed

43.

Waarsing J, Day J, Van der Linden J, Ederveen A, Spanjers C, De Clerck N, Sasov A, Verhaar JA, Weinans H (2004) Detecting and tracking local changes in the tibiae of individual rats: a novel method to analyse longitudinal in vivo micro-CT data. Bone 34:163–169CrossRefPubMed

44.

Waarsing JH, Day JS, Verhaar JA, Ederveen AG, Weinans H (2006) Bone loss dynamics result in trabecular alignment in aging and ovariectomized rats. J Orthop Res 24:926–935. doi:10.1002/jor.20063 CrossRefPubMed

45.

Ammann P (2002) Determining factors of bone mechanical resistance. Therapie 58:403–407. doi:10.2515/Therapie:2003065 CrossRef

46.

Viguet-Carrin S, Garnero P, Delmas P (2006) The role of collagen in bone strength. Osteoporos Int 17:319–336. doi:10.1007/s00198-005-2035-9 CrossRefPubMed

47.

Eyre DR, Dickson I, Van Ness K (1988) Collagen cross-linking in human bone and articular cartilage. Age-related changes in the content of mature hydroxypyridinium residues. Biochem J 252:495–500CrossRefPubMedPubMedCentral

48.

Bailey A, Wotton S, Sims T, Thompson P (1993) Biochemical changes in the collagen of human osteoporotic bone matrix. Connect Tissue Res 29:119–132CrossRefPubMed

49.

Fujimoto K, Kiyosaki T, Mitsui N, Mayahara K, Omasa S, Suzuki N, Shimizu N (2010) Low-intensity laser irradiation stimulates mineralization via increased BMPs in MC3T3-E1 cells. Lasers Surg Med 42:519–526. doi:10.1002/lsm.20880/full CrossRefPubMed

50.

Donoso O, Pino AM, Seitz G, Osses N, Rodríguez JP (2015) Osteoporosis-associated alteration in the signaling status of BMP-2 in human MSCs under adipogenic conditions. J Cell Biochem 116:1267–1277. doi:10.1002/jcb.25082 CrossRefPubMed

51.

Bayat M (2014) The necessity for increased attention to pulsed low-level laser therapy. Photomed Laser Surg 32(8):427–428. doi:10.1089/pho.2014.9858 CrossRefPubMed

Titel: Evaluation of the effects of photobiomodulation on vertebras in two rat models of experimental osteoporosis
verfasst von: Mohammadjavad Fredoni
Mahdi Ghatrehsamani
Mohammad-amin Abdollahifar
Sahar Bayat
Mohammad Bayat
Publikationsdatum: 19.07.2017
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 7/2017
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-017-2278-7

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 7/2017

Discrimination model applied to urinalysis of patients with diabetes and hypertension aiming at diagnosis of chronic kidney disease by Raman spectroscopy

Nevus spilus: treatment with fractional CO2 laser in combination with MedLite C6 laser: a preliminary study

Comment on “Effectiveness of antimicrobial photodynamic therapy (AmPDT) on Staphylococcus aureus using phenothiazinecompound with red laser”

Noninvasive assessment of cutaneous alterations in mice exposed to whole body gamma irradiation using optical imaging techniques

Effects of PBM in different energy densities and irradiance on maintaining cell viability and proliferation of pulp fibroblasts from human primary teeth

Laser treatments of active acne