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06.02.2018 | Original Article

Evaluation of the genotoxic effects of formocresol application in vital pulp therapy of primary teeth: a clinical study and meta-analysis

verfasst von: Arnoldo Vasconcelos de Alencar Filho, Valdeci Elias dos Santos Junior, Merilane da Silva Calixto, Neide Santos, Monica Vilela Heimer, Aronita Rosenblatt

Erschienen in: Clinical Oral Investigations | Ausgabe 7/2018

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Abstract

Objectives

This in vivo research investigated whether pulp treatments using formocresol for 7 days would cause mutagenic changes in children’s lymphocytes.

Materials and methods

The mutagenicity was tested in lymphocyte cultures established from the peripheral blood of children living in Brazil. The samples consisted of 2000 cells from teeth undergoing formocresol pulpotomies in which the formocresol pellet was sealed in the primary tooth for 7 days. It was removed on the seventh day, the base was placed, and the tooth was restored. Two venous blood samples (6–8 ml) were collected from each child; the first was prior to pulp therapy, and the second was 7 days later. Two thousand metaphases were analyzed. The level of significance adopted for the statistics was P < 0.05, and a random effects meta-analysis was performed combining this and two previous studies.

Results

There was no significant difference found in the metaphase analysis between the blood samples taken before and after the pulpotomy treatment (Wilcoxon signed rank test); however, the meta-analysis showed a significant difference between the combined studies.

Conclusions

This study did not reveal any mutagenic effects, but based on the combined meta-analysis, we recommend the careful use of formocresol.

Clinical relevance

This research helps to bring scientific evidence of the safe use of formocresol in deciduous pulpotomy treatments.

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Titel: Evaluation of the genotoxic effects of formocresol application in vital pulp therapy of primary teeth: a clinical study and meta-analysis
verfasst von: Arnoldo Vasconcelos de Alencar Filho
Valdeci Elias dos Santos Junior
Merilane da Silva Calixto
Neide Santos
Monica Vilela Heimer
Aronita Rosenblatt
Publikationsdatum: 06.02.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 7/2018
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2352-y

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Evaluation of the genotoxic effects of formocresol application in vital pulp therapy of primary teeth: a clinical study and meta-analysis

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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