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11.06.2024 | Original Article

Evaluation of the Role of Myofibroblast and Fibronectin in the Aetiopathogenesis of Cholesteatoma

verfasst von: Ramya Ravindra Kamath, Prahlada Nayaka Basavanthappa, B. J. Bindu, C. Narayana Murthy, Gouri Priya Rajeev, S. Raisa

Erschienen in: Indian Journal of Otolaryngology and Head & Neck Surgery | Ausgabe 5/2024

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Abstract

Background

Primary acquired Cholesteatoma is a complex issue for otolaryngologists, with its development mechanisms still unclear due to the intricate anatomy of this region. It’s aetiopathogenesis remains poorly understood and this aggressive clinical condition often leads to various complications. Recent research explores myofibroblast and fibronectin's potential roles in pathomechanisms of Cholesteatoma.

Objective

To determine and analyze the role of myofibroblast and fibronectin in the aetiopathogenesis of Cholesteatoma.

Methodology

In a cross-sectional study at a tertiary care hospital, 30 patients with chronic suppurative otitis media with cholesteatoma were surgically treated, and intraoperative biopsy specimens were collected. These specimens were processed and subjected to histopathological examination, including immunohistochemical staining with Alpha-smooth muscle actin and anti-fibronectin antibody to identify myofibroblast and fibronectin presence. The data were then analyzed to investigate the aetiopathogenesis of cholesteatoma in this cohort.

Results

On immunostaining, 25 blocks (83.33%) were positively stained for Alpha-SMA (p-value—0.0007), whereas 29 blocks (96.67%) were positively stained for fibronectin (p-value < 0.0001), suggesting a statistically significant association between the presence of both myofibroblast and fibronectin with cholesteatoma perimatrix. Additionally, a statistically significant association was noted between complications and positive staining for myofibroblast (p-value − 0.0415) and positive staining for fibronectin (p-value—0.0254).

Conclusions

Our study indicates that Cholesteatoma retraction and progression are driven by myofibroblast and fibronectin mechanisms, and also links them to disease severity. This understanding opens avenues for innovative diagnostics and treatments targeting these biomarkers.
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Metadaten
Titel
Evaluation of the Role of Myofibroblast and Fibronectin in the Aetiopathogenesis of Cholesteatoma
verfasst von
Ramya Ravindra Kamath
Prahlada Nayaka Basavanthappa
B. J. Bindu
C. Narayana Murthy
Gouri Priya Rajeev
S. Raisa
Publikationsdatum
11.06.2024
Verlag
Springer India
Erschienen in
Indian Journal of Otolaryngology and Head & Neck Surgery / Ausgabe 5/2024
Print ISSN: 2231-3796
Elektronische ISSN: 0973-7707
DOI
https://doi.org/10.1007/s12070-024-04784-8

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