Skip to main content
Erschienen in: Comparative Clinical Pathology 3/2017

15.02.2017 | Original Article

Evaluation of wound healing activity of St. John’s Wort (Hypericum perfoliatum) in horses

verfasst von: Giudice Elisabetta, Crinò Chiara, Salerno Gaetano, Rizzo Maria, Levanti Maria, Di Pietro Simona

Erschienen in: Comparative Clinical Pathology | Ausgabe 3/2017

Einloggen, um Zugang zu erhalten

Abstract

St. John’s Wort (SJW) is an herbaceous medical plant. Since ancient times, SJW has been used to treat different kinds of mental and physical diseases and, for its antiseptic, anti-inflammatory and antibacterial properties. The aim of this study was to evaluate the wound healing activity of a phytotherapeutic product from St. John’s Wort (SJW), herbaceous medical perennial plant, on skin lesions of different origins, in horses housed in Sicily (Italy). Six horses of different breeds, age and attitude were enrolled in the study. All the lesions were treated with an oil prepared from the aerial parts of Hypericum perfoliatum (H. perfoliatum), macerated in oil under the sun. Topical application of the oil determined a significant improvement of skin lesions in all the horses involved in the study. Wound healing begun (Time1) in a period ranging from 2 days to 2 weeks (mean ± SD, 4 ± 1.89 days). The treatment resulted in complete resolution of wounds (Time2) in a period between 1 and 5 weeks (mean ± SD, 14.5 ± 8.2 days), while hair re-growth (Time3) was completed in period between 25 days and 2 months (mean ± SD, 33.25 ± 12.58 days). This study has certainly given a scientific point of view to a century of empiricism about the use of SJW in veterinary practice. The findings of this study seem to suggest that SJW may be considered an “all-in-one” remedy for wound healing, in order to make the management of skin injuries easier even for severe and complicated lesions.
Literatur
Zurück zum Zitat Benkiki N, Kabouche Z, Tillequin F, Vérité P, Chosson E, Seguin E (2003) A new polyisoprenylated phloroglucinol derivative from Hypericum perfoliatum (Clusiaceae). Z Naturforsch C 58:655–658CrossRefPubMed Benkiki N, Kabouche Z, Tillequin F, Vérité P, Chosson E, Seguin E (2003) A new polyisoprenylated phloroglucinol derivative from Hypericum perfoliatum (Clusiaceae). Z Naturforsch C 58:655–658CrossRefPubMed
Zurück zum Zitat Caccia S, Gobbi M (2010) St’s John Wort components and brain: uptake, concentrations reached and the mechanisms underlying pharmacological effects. Cur Drug Metab 10:1055–1065CrossRef Caccia S, Gobbi M (2010) St’s John Wort components and brain: uptake, concentrations reached and the mechanisms underlying pharmacological effects. Cur Drug Metab 10:1055–1065CrossRef
Zurück zum Zitat Campanini E (2012) Dizionario di fitoterapia e piante medicinali, 3rd edn. Tecniche Nuove, Milano Campanini E (2012) Dizionario di fitoterapia e piante medicinali, 3rd edn. Tecniche Nuove, Milano
Zurück zum Zitat Castro FCB, Magre A, Cherpinski R, Zelante PM, Neves LMG, Esquisatto MAM, Mendonça FAS, Santos GMT (2012) Effects of microcurrent application alone or in combination with topical Hypericum perforatum L. and Arnica Montana L. on surgically induced wound healing in Wistar rats. Homeopathy 101:147–153CrossRefPubMed Castro FCB, Magre A, Cherpinski R, Zelante PM, Neves LMG, Esquisatto MAM, Mendonça FAS, Santos GMT (2012) Effects of microcurrent application alone or in combination with topical Hypericum perforatum L. and Arnica Montana L. on surgically induced wound healing in Wistar rats. Homeopathy 101:147–153CrossRefPubMed
Zurück zum Zitat Del Monte D, De Martino L, Marandino A, Fratianni F, Nazzaro F, De Feo V (2015) Phenolic content, antimicrobial and antioxidant activities of Hypericum perfoliatum L. Ind Corps Prod 74:342–347CrossRef Del Monte D, De Martino L, Marandino A, Fratianni F, Nazzaro F, De Feo V (2015) Phenolic content, antimicrobial and antioxidant activities of Hypericum perfoliatum L. Ind Corps Prod 74:342–347CrossRef
Zurück zum Zitat Dikmen M, Öztürk Y, Sagratini G, Ricciutelli M, Vittori S, Maggi F (2011) Evaluation of the wound healing potentials of two subspecies of Hypericum perforatum on cultured NIH3T3 fibroblasts. Phytother Res 25:208–214PubMed Dikmen M, Öztürk Y, Sagratini G, Ricciutelli M, Vittori S, Maggi F (2011) Evaluation of the wound healing potentials of two subspecies of Hypericum perforatum on cultured NIH3T3 fibroblasts. Phytother Res 25:208–214PubMed
Zurück zum Zitat do Rego JC, Benkiki N, Chosson E, Kabouche Z, Seguin E, Costentin E (2007) Antidepressant-like effect of hyperfoliatin, a polyisoprenylated phloroglucinol derivative from Hypericum perfoliatum (Clusiaceae) is associated with an inhibition of neuronal monoamines uptake. Eur J Pharmacol 569:197–203CrossRefPubMed do Rego JC, Benkiki N, Chosson E, Kabouche Z, Seguin E, Costentin E (2007) Antidepressant-like effect of hyperfoliatin, a polyisoprenylated phloroglucinol derivative from Hypericum perfoliatum (Clusiaceae) is associated with an inhibition of neuronal monoamines uptake. Eur J Pharmacol 569:197–203CrossRefPubMed
Zurück zum Zitat Fukunaga K, Orito K (2012) Time-course of St John’s Wort on the pharmacokinetics of cyclosporine in dogs: interactions between herbal extracts and drugs. J Vet Pharmacol Ther 35:446–451CrossRefPubMed Fukunaga K, Orito K (2012) Time-course of St John’s Wort on the pharmacokinetics of cyclosporine in dogs: interactions between herbal extracts and drugs. J Vet Pharmacol Ther 35:446–451CrossRefPubMed
Zurück zum Zitat Galeotti N, Vivoli E, Bilia AR, Souto EB, Calpena AC, Garcia ML (2010) St. John’s Wort reduces neuropathic pain through a hypericin-mediated inhibition of the protein kinase C gamma and epsilon activity. Biochem Pharmacol 79:1327–1336CrossRefPubMed Galeotti N, Vivoli E, Bilia AR, Souto EB, Calpena AC, Garcia ML (2010) St. John’s Wort reduces neuropathic pain through a hypericin-mediated inhibition of the protein kinase C gamma and epsilon activity. Biochem Pharmacol 79:1327–1336CrossRefPubMed
Zurück zum Zitat Groning R, Breitkreutz J, Muller RS (2003) Physico-chemical interactions between extracts of Hypericum perforatum L. and drugs. Eur J Pharm Biopharm 56:231–236CrossRefPubMed Groning R, Breitkreutz J, Muller RS (2003) Physico-chemical interactions between extracts of Hypericum perforatum L. and drugs. Eur J Pharm Biopharm 56:231–236CrossRefPubMed
Zurück zum Zitat Heimann M, Janda J, Sigurdardottir OG, Svansson V, Klukowska J, Von Tscharner C, Doherr M, Broström H, Andersson LS, Einarsson S, Marti E, Torsteinsdottir S (2011) Skin-infiltrating T cells and cytokine expression in Icelandic horses affected with insect bite hypersensitivity: a possible role for regulatory T cells. Vet Immunol Immunopathol 140:63–74CrossRefPubMed Heimann M, Janda J, Sigurdardottir OG, Svansson V, Klukowska J, Von Tscharner C, Doherr M, Broström H, Andersson LS, Einarsson S, Marti E, Torsteinsdottir S (2011) Skin-infiltrating T cells and cytokine expression in Icelandic horses affected with insect bite hypersensitivity: a possible role for regulatory T cells. Vet Immunol Immunopathol 140:63–74CrossRefPubMed
Zurück zum Zitat Hostanska K, Rostock M, Melzer J, Baumgartner S, Saller R (2012) A homeopathic remedy from arnica, marigold, St. John’s Wort and comfrey accelerates in vitro wound scratch closure of NIH 3T3 fibroblasts. Complement Altern Med 12:100–109CrossRef Hostanska K, Rostock M, Melzer J, Baumgartner S, Saller R (2012) A homeopathic remedy from arnica, marigold, St. John’s Wort and comfrey accelerates in vitro wound scratch closure of NIH 3T3 fibroblasts. Complement Altern Med 12:100–109CrossRef
Zurück zum Zitat Khan AU, Gilani AH, Naieeb U-R (2011) Pharmacological studies on Hypericum perforatum fractions and constituents. Pharm Biol 49:46–56CrossRefPubMed Khan AU, Gilani AH, Naieeb U-R (2011) Pharmacological studies on Hypericum perforatum fractions and constituents. Pharm Biol 49:46–56CrossRefPubMed
Zurück zum Zitat Kiyan S, Uyanikgil Y, Altunci YA, Çavuşoğlu T, Uyanikgil EOC, Karabey F (2015) Investigation of acute effects of Hypericum perforatum (St. John’s Wort-Kantaron) treatment in experimental thermal burns and comparison with silver sulfadiazine treatment. Ulus Travma Acil Cerrahi Derg 21:323–336PubMed Kiyan S, Uyanikgil Y, Altunci YA, Çavuşoğlu T, Uyanikgil EOC, Karabey F (2015) Investigation of acute effects of Hypericum perforatum (St. John’s Wort-Kantaron) treatment in experimental thermal burns and comparison with silver sulfadiazine treatment. Ulus Travma Acil Cerrahi Derg 21:323–336PubMed
Zurück zum Zitat Koeberle A, Rossi A, Bauer J, Dehm F, Verotta L, Northoff H, Sautebin L, Werz O (2011) Hyperforin, an anti-inflammatory constituent from St. John’s Wort, inhibits microsomal prostaglandin E2 synthase-1 and suppresses prostaglandin E2 formation in vivo. Front Pharmacol 2:1–10CrossRef Koeberle A, Rossi A, Bauer J, Dehm F, Verotta L, Northoff H, Sautebin L, Werz O (2011) Hyperforin, an anti-inflammatory constituent from St. John’s Wort, inhibits microsomal prostaglandin E2 synthase-1 and suppresses prostaglandin E2 formation in vivo. Front Pharmacol 2:1–10CrossRef
Zurück zum Zitat Lu WD, Atkins WM (2004) A novel antioxidant role for ligand behavior of glutathione S-transferases: attention of the photodynamic effects of hypericin. Biochemistry-US 43:12761–12769CrossRef Lu WD, Atkins WM (2004) A novel antioxidant role for ligand behavior of glutathione S-transferases: attention of the photodynamic effects of hypericin. Biochemistry-US 43:12761–12769CrossRef
Zurück zum Zitat Mainetti S, Carnevalli F (2013) An experience with paediatric burn wounds treated with a plant-derived wound therapeutic. J Wound Care 22:681–689CrossRefPubMed Mainetti S, Carnevalli F (2013) An experience with paediatric burn wounds treated with a plant-derived wound therapeutic. J Wound Care 22:681–689CrossRefPubMed
Zurück zum Zitat Meinke MC, Schanzer S, Haag SF, Casetti F, Muller LM, Wofle U, Kleemann A, Lademann J, Schempp CM (2012) In vivo photoprotective and anti-inflammatory effect of hyperforin is associated with high antioxidant activity in vitro and ex vivo. Eur J Pharm Biopharm 81:346–350CrossRefPubMed Meinke MC, Schanzer S, Haag SF, Casetti F, Muller LM, Wofle U, Kleemann A, Lademann J, Schempp CM (2012) In vivo photoprotective and anti-inflammatory effect of hyperforin is associated with high antioxidant activity in vitro and ex vivo. Eur J Pharm Biopharm 81:346–350CrossRefPubMed
Zurück zum Zitat Montoya A, Daza A, Muñoz D, Ríos K, Taylor V, Cedeño D, Vélez I, Echeverri F, Robledo SM (2015) Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies. Antimicrob Agents Ch 59:5804–5813CrossRef Montoya A, Daza A, Muñoz D, Ríos K, Taylor V, Cedeño D, Vélez I, Echeverri F, Robledo SM (2015) Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies. Antimicrob Agents Ch 59:5804–5813CrossRef
Zurück zum Zitat Nogueira T, Medeiros MA, Marcelo-Curto MJ, Garcia-Perez BE, Luna-Herrera J, Costa MC (2013) Profile of antimicrobial potential of fifteen Hypericum species from Portugal. Ind Crop Prod 47:126–131CrossRef Nogueira T, Medeiros MA, Marcelo-Curto MJ, Garcia-Perez BE, Luna-Herrera J, Costa MC (2013) Profile of antimicrobial potential of fifteen Hypericum species from Portugal. Ind Crop Prod 47:126–131CrossRef
Zurück zum Zitat Onoue S, Seto Y, Ochi M, Inoue R, Ito H, Hatano T, Yamada S (2011) In vitro photochemical and phototoxicological characterization of major constituents in St. John’s Wort (Hypericum perforatum) extracts. Phytochemistry 72:1814–1820CrossRefPubMed Onoue S, Seto Y, Ochi M, Inoue R, Ito H, Hatano T, Yamada S (2011) In vitro photochemical and phototoxicological characterization of major constituents in St. John’s Wort (Hypericum perforatum) extracts. Phytochemistry 72:1814–1820CrossRefPubMed
Zurück zum Zitat Prisăcaru AI, Andriţoiu CV, Andriescu C, Hăvârneanu EC, Popa M, Motoc AGM, Sava A (2013) Evaluation of the wound-healing effect of a novel Hypericum perforatum ointment in skin injury. Romanian J Morphol Embryol 54:1053–1059 Prisăcaru AI, Andriţoiu CV, Andriescu C, Hăvârneanu EC, Popa M, Motoc AGM, Sava A (2013) Evaluation of the wound-healing effect of a novel Hypericum perforatum ointment in skin injury. Romanian J Morphol Embryol 54:1053–1059
Zurück zum Zitat Saddiqe Z, Naeem I, Maimoona A (2010) A review of the antibacterial activity of Hypericum perforatum L. J Ethnopharmacol 131:511–521CrossRefPubMed Saddiqe Z, Naeem I, Maimoona A (2010) A review of the antibacterial activity of Hypericum perforatum L. J Ethnopharmacol 131:511–521CrossRefPubMed
Zurück zum Zitat Sayar H, Gergerlioglu N, Seringec N, Ozturk P, Bulbuloglu E, Karaba G (2014) Comparison of efficacy of topical phenytoin with hypericin in second-degree burn wound healing: an experimental study in rats. Med Sci Monit Basic Res 20:36–46CrossRefPubMedPubMedCentral Sayar H, Gergerlioglu N, Seringec N, Ozturk P, Bulbuloglu E, Karaba G (2014) Comparison of efficacy of topical phenytoin with hypericin in second-degree burn wound healing: an experimental study in rats. Med Sci Monit Basic Res 20:36–46CrossRefPubMedPubMedCentral
Zurück zum Zitat Singh S, Sarma S, Katiyar SP, Das M, Bhardwaj R, Sundar D, Dubey VK (2015) Probing the molecular mechanism of hypericin-induced parasite death provides insight in the role of spermidine beyond redox metabolism in Leishmania donovani. Antimicrob Agents Chemother 59:15–24CrossRefPubMed Singh S, Sarma S, Katiyar SP, Das M, Bhardwaj R, Sundar D, Dubey VK (2015) Probing the molecular mechanism of hypericin-induced parasite death provides insight in the role of spermidine beyond redox metabolism in Leishmania donovani. Antimicrob Agents Chemother 59:15–24CrossRefPubMed
Zurück zum Zitat Stevens PF (2006) Clusiaceae-Guttiferae. In: Kubitzki K (ed) The families and genera of vascular plants: Vol. IX, Flowering Plants, Eudicots. Springer, Berlin/Heidelberg/New York, pp 48–66 Stevens PF (2006) Clusiaceae-Guttiferae. In: Kubitzki K (ed) The families and genera of vascular plants: Vol. IX, Flowering Plants, Eudicots. Springer, Berlin/Heidelberg/New York, pp 48–66
Zurück zum Zitat Suntar IP, Akkol EK, Yilmazer D, Baykal T, Kirmizibekmez H, Alper M, Yeşilada E (2010) Investigations on the in vivo wound healing potential of Hypericum perforatum L. J Ethnopharmacol 127:468–477CrossRefPubMed Suntar IP, Akkol EK, Yilmazer D, Baykal T, Kirmizibekmez H, Alper M, Yeşilada E (2010) Investigations on the in vivo wound healing potential of Hypericum perforatum L. J Ethnopharmacol 127:468–477CrossRefPubMed
Zurück zum Zitat Tanideh N, Namazi F, Andisheh Tadbir A, Ebrahimi H, Koohi-Hosseinabadi O (2014) Comparative assessment of the therapeutic effects of the topical and systemic forms of Hypericum perforatum extract on induced oral mucositis in golden hamsters. Int J Oral Maxillofac 43:1286–1292CrossRef Tanideh N, Namazi F, Andisheh Tadbir A, Ebrahimi H, Koohi-Hosseinabadi O (2014) Comparative assessment of the therapeutic effects of the topical and systemic forms of Hypericum perforatum extract on induced oral mucositis in golden hamsters. Int J Oral Maxillofac 43:1286–1292CrossRef
Zurück zum Zitat Tracey AK, Alcott CJ, Schleining JA, Safayi S, Zaback PC, Hostetter JM, Reinertson EL (2014) The effects of topical oxygen therapy on equine distal limb dermal wound healing. Can Vet J55:1146–1152 Tracey AK, Alcott CJ, Schleining JA, Safayi S, Zaback PC, Hostetter JM, Reinertson EL (2014) The effects of topical oxygen therapy on equine distal limb dermal wound healing. Can Vet J55:1146–1152
Zurück zum Zitat Vazzana M, Macedo AS, Santini A, Faggio C, Souto EB (2014) Novel neuroprotective formulations based on St. John’s Wort extract. J Food Res 3:3–7CrossRef Vazzana M, Macedo AS, Santini A, Faggio C, Souto EB (2014) Novel neuroprotective formulations based on St. John’s Wort extract. J Food Res 3:3–7CrossRef
Zurück zum Zitat Wang Y, Shi X, Qi Z (2010) Hypericin prolongs action potential duration in hippocampal neurons by acting on K+ channels. Br J Pharmacol 159:1402–1407CrossRefPubMedPubMedCentral Wang Y, Shi X, Qi Z (2010) Hypericin prolongs action potential duration in hippocampal neurons by acting on K+ channels. Br J Pharmacol 159:1402–1407CrossRefPubMedPubMedCentral
Zurück zum Zitat Wolfle U, Seelinger G, Schempp CM (2014) Topical application of St. Johnʼs Wort (Hypericum perforatum). Planta Med 80:109–120CrossRefPubMed Wolfle U, Seelinger G, Schempp CM (2014) Topical application of St. Johnʼs Wort (Hypericum perforatum). Planta Med 80:109–120CrossRefPubMed
Metadaten
Titel
Evaluation of wound healing activity of St. John’s Wort (Hypericum perfoliatum) in horses
verfasst von
Giudice Elisabetta
Crinò Chiara
Salerno Gaetano
Rizzo Maria
Levanti Maria
Di Pietro Simona
Publikationsdatum
15.02.2017
Verlag
Springer London
Erschienen in
Comparative Clinical Pathology / Ausgabe 3/2017
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-017-2426-2

Weitere Artikel der Ausgabe 3/2017

Comparative Clinical Pathology 3/2017 Zur Ausgabe

Neu im Fachgebiet Pathologie