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01.12.2012 | Research | Ausgabe 1/2012 Open Access

Malaria Journal 1/2012

Ex vivo drug sensitivity profiles of Plasmodium falciparum field isolates from Cambodia and Thailand, 2005 to 2010, determined by a histidine-rich protein-2 assay

Zeitschrift:
Malaria Journal > Ausgabe 1/2012
Autoren:
Stuart D Tyner, Chanthap Lon, Youry Se, Delia Bethell, Doung Socheat, Harald Noedl, Darapiseth Sea, Wichai Satimai, Kurt Schaecher, Wiriya Rutvisuttinunt, Mark M Fukuda, Suwanna Chaorattanakawee, Kritsanai Yingyuen, Siratchana Sundrakes, Panjaporn Chaichana, Piyaporn Saingam, Nillawan Buathong, Sabaithip Sriwichai, Soklyda Chann, Ans Timmermans, David L Saunders, Douglas S Walsh
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1475-2875-11-198) contains supplementary material, which is available to authorized users.
Stuart D Tyner, Chanthap Lon contributed equally to this work.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

Study designs, oversight, data interpretation, manuscript preparation: CL, ST, DS (Dr Socheat), YS, HN, DB, KS, WR, WS, MF, SC (Dr. Suwanna), AT, DS (Dr Saunders), DW Subject recruitment, interactions: DS (Dr Sea), YS, SC (Ms. Chann), NB, SS (Ms Sabaithip) Conducted experiments, summarized data: ST, KS, WR, SC, KY, SS (Ms Siratchana), PC. All authors read and approved the final manuscript.

Abstract

Background

In vitro drug susceptibility assay of Plasmodium falciparum field isolates processed “immediate ex vivo” (IEV), without culture adaption, and tested using histidine-rich protein-2 (HRP-2) detection as an assay, is an expedient way to track drug resistance.

Methods

From 2005 to 2010, a HRP-2 in vitro assay assessed 451 P. falciparum field isolates obtained from subjects with malaria in western and northern Cambodia, and eastern Thailand, processed IEV, for 50% inhibitory concentrations (IC50) against seven anti-malarial drugs, including artesunate (AS), dihydroartemisinin (DHA), and piperaquine.

Results

In western Cambodia, from 2006 to 2010, geometric mean (GM) IC50 values for chloroquine, mefloquine, quinine, AS, DHA, and lumefantrine increased. In northern Cambodia, from 2009–2010, GM IC50 values for most drugs approximated the highest western Cambodia GM IC50 values in 2009 or 2010.

Conclusions

Western Cambodia is associated with sustained reductions in anti-malarial drug susceptibility, including the artemisinins, with possible emergence, or spread, to northern Cambodia. This potential public health crisis supports continued in vitro drug IC50 monitoring of P. falciparum isolates at key locations in the region.
Zusatzmaterial
Authors’ original file for figure 1
12936_2012_2111_MOESM1_ESM.pdf
Authors’ original file for figure 2
12936_2012_2111_MOESM2_ESM.pdf
Literatur
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