Background
Fundamental characteristics and physiological functions of exosomes
Exosomes’ functions in cancer
Technology for exosome isolation and identification and analysis of their proteins
Methods for isolation exosomes
Ultracentrifugation (UC)
Size exclusion chromatography (SEC)
Polymer precipitation
Immunocapture assays
Microfluidic technologies
Strategies for exosome identification and exosomal protein analysis
The application of exosomal proteins in tumor diagnosis
Exosomal proteins | Tumor | Body fluid | Isolation method | Detection method | Year | Ref |
---|---|---|---|---|---|---|
NY-ESO-1 | Lung | Plasma | Extracellular Vesicle Array | Extracellular Vesicle Array | 2016 | [66] |
PKG1, RALGAPA2, NFX1, TJP2 | Breast | Plasma | Ultracentrifugation | Parallel reaction monitoring (PRM) | 2017 | [2] |
Her2 | Breast | Plasma | Microfluidic chip | Microfluidic chip | 2017 | [70] |
Glypican-1 | Breast | Serum | Ultracentrifugation and FACS | FACS | 2016 | [5] |
Glypican-1 | Pancreatic | Serum | Ultracentrifugation and FACS | Flow-cytometry and ELISA | 2016 | [5] |
Glypican-1 | Colorectal | Plasma | Immunocapture assays | Flow-cytometry | 2017 | [76] |
CEA | Colorectal | Serum | Polymer precipitation | ELISA | 2017 | [46] |
AMPN VNN1, PIGR | Cholangiocarcinoma | Serum | Ultracentrifugation | Western blot | 2016 | [77] |
PSA | Prostate | Plasma | Filtration and ultracentrifugation | ELISA and nanoscale flow-cytometry | 2017 | [41] |
GGT1 | Prostate | Serum | Ultracentrifugation/DGC/SEC | Western blot and fluorescent probe | 2017 | [80] |
CD24, EpCAM, CA-125 | Ovarian | Plasma | Microfluidic ExoSearch Chip | Microfluidic ExoSearch Chip | 2016 | [86] |