Background
Methods
Bacterial strains
Antimicrobial susceptibility testing
Molecular characterization of 16S rRNA methyltransferase genes
Target primers | Sequence (Forward& reverse-5'-3') | Amplicon size (bp) | Reference |
---|---|---|---|
armA | GGTGCGAAAACAGTCGTAGT | 1153 | [22] |
TCCTCAAATATCCTCTATGT | |||
rmtA | CTAGCGTCCATCCTTTCCTC | 635 | [22] |
TTTGCTTCCATGCCCTTGCC | |||
rmtB | GGAATTCCATATGAACATCAACGATGCC | 756 | [22] |
CCGCTCGAGTCCATTCTTTTTTATCAAGT | |||
rmtC | CGAAGAAGTAACAGCCAAAG | 1000 | [22] |
GCTAGAGTCAAGCCAGAAAA | |||
rmtD | TCATTTTCGTTTCAGCAC | 744 | [22] |
AAACATGAGCGAACTGAAGG | |||
npmA | CGGGATCCAAGCACTTTCATACTGACG | 981 | [22] |
CGGAATTCCAATTTTGTTCTTATTAGC | |||
rmtE | ATGAATATTGATGAAATGGTTGC | 818 | [23] |
TGATTGATTTCCTCCGTTTTTG | |||
rmtF | GCGATACAGAAAACCGAAGG | 589 | [24] |
ACCAGTCGGCATAGTGCTTT | |||
rmtG | AAATACCGCGATGTGTGTCC | 250 | [25] |
ACACGGCATCTGTTTCTTCC | |||
rmtH | AATGACCATTGAACAGGCAGC | 760 | [26] |
TCAAGCTGGGTTTGGCTGGA |
Determination of co-existing ESBLs and carbapenemases
Horizontal transferability assay
Plasmid incompatibility typing
Multilocus sequence typing (MLST)
Results
Detection of 16S rRNA methyltransferase genes
Sl. no | 16S rRNA methyltransferase genes harbouring isolates | Co-existing ESBLs | Co- existing carbapenemase | Sequence Types | ||
---|---|---|---|---|---|---|
1 | rmtC (n = 12) | CTX-M-15(n = 9) | - | NDM (n = 1) | - | ST3906 |
2 | armA(n = 11) | CTX-M-15(n = 3) | VEB (n = 1) | NDM (n = 2) | OXA-48 (n = 1) | ST4410 |
3 | rmtD(n = 9) | CTX-M-15(n = 5) | - | NDM (n = 2) | OXA-48 (n = 1) | ST3906 |
4 | rmtF (n = 8) | CTX-M-15(n = 2) | - | NDM (n = 1) | OXA-48 (n = 1) | ST1341 |
5 | rmtB (n = 6) | CTX-M-15(n = 3) | - | NDM (n = 2) | OXA-48 (n = 2) | Unknown |
6 | npmA (n = 5) | CTX-M-15(n = 2) | VEB (n = 1) | NDM (n = 1) | - | Unknown |
7 | rmtH (n = 4) | CTX-M-15(n = 7) | PER (n = 1) | NDM (n = 3) | OXA-48 (n = 1) | ST4410 |
8 | rmtE (n = 1) | CTX-M-15(n = 1) | - | NDM (n = 2) | - | Unknown |
9 | rmtG(n = 1) | CTX-M-15(n = 1) | - | NDM (n = 3) | OXA-48 (n = 1) | Unknown |
10 | armA +rmtF(n = 6) | CTX-M-15(n = 3) | - | NDM (n = 1) | - | ST4410 |
11 | rmtG+ rmtH (n = 3) | CTX-M-15(n = 2) | - | NDM (n = 1) | - | ST4410 |
12 | armA+rmtE(n = 3) | CTX-M-15(n = 1) | - | NDM (n = 2) | - | ST4410 |
13 | rmtC+ rmtD (n = 3) | CTX-M-15(n = 1) | - | - | OXA-48 (n = 1) | ST3906 |
14 | armA+rmtA (n = 2) | CTX-M-15(n = 1) | - | NDM (n = 1) | - | ST4410 |
15 | armA+rmtF+rmtC+rmtD (n = 3) | CTX-M-15(n = 1) | - | NDM (n = 1) | OXA-48 (n = 1) | ST3906 |