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Erschienen in: International Journal of Hematology 5/2020

14.09.2020 | Guideline

Expert consensus regarding standardization of sample preparation for clotting time assays

verfasst von: Masahiro Ieko, Yutaka Komiyama, Satoshi Yamazaki, Hisako Katagiri, Chisato Shimazu, Sumiyoshi Naito, Masato Matsuda, Yoichi Yuki, Kazuyoshi Miyata, Rika Arai, Osamu Kumano, Mari Emmi, Tatsuya Yoshida, Hideaki Tanaka, Toshihiko Okuhara, Tetsuji Yamashita, Yasuji Sekine, Masaru Homma, Yohko Kawai, Kaoru Tohyama, Masahiro Ieko, Yutaka Komiyama, Satoshi Yamazaki, Hisako Katagiri, Chisato Shimazu, Sumiyoshi Naito, Masato Matsuda, Yoichi Yuki, Kazuyoshi Miyata, Rika Arai, Osamu Kumano, Mari Emmi, Tatsuya Yoshida, Hideaki Tanaka, Toshihiko Okuhara, Tetsuji Yamashita, Yasuji Sekine, Masaru Homma, Yohko Kawai, Kaoru Tohyama, The working group for standardization of sample preparation for clotting time assays, Japanese society for laboratory hematology.

Erschienen in: International Journal of Hematology | Ausgabe 5/2020

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Abstract

Accurate clotting time assay results are vital, as the test is employed to indicate the amount of oral anticoagulant to be prescribed, while it is also used for screening the hemorrhagic and thrombotic diseases. The procedure chosen for preparation of a patient blood sample including centrifugation can contribute to significant differences in the results obtained. Thus, for the purpose of proposing a standardized method to appropriately prepare blood samples prior to assay, the Japanese Society of Laboratory Hematology organized the Working Group for Standardization of Sample Preparation for Clotting Time Assays (WG). Following reviews of previously announced guidelines and original experimental results, consensus was obtained by the WG, with the main findings as follows. (1) The recommended anticoagulant in the blood collection tube is sodium citrate solution at 0.105–0.109 M (3.13–3.2%). (2) Whole blood samples should be stored at room temperature (18–25 ˚C) within 1 h of collection from the patient. (3) For plasma preparation, centrifugation at 1500 × g should be performed for at least 15 min or at 2000 × g for at least 10 min at room temperature. (4) After the plasma sample is prepared, it should be stored at room temperature and assayed within 4 h.
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Metadaten
Titel
Expert consensus regarding standardization of sample preparation for clotting time assays
verfasst von
Masahiro Ieko
Yutaka Komiyama
Satoshi Yamazaki
Hisako Katagiri
Chisato Shimazu
Sumiyoshi Naito
Masato Matsuda
Yoichi Yuki
Kazuyoshi Miyata
Rika Arai
Osamu Kumano
Mari Emmi
Tatsuya Yoshida
Hideaki Tanaka
Toshihiko Okuhara
Tetsuji Yamashita
Yasuji Sekine
Masaru Homma
Yohko Kawai
Kaoru Tohyama
Masahiro Ieko
Yutaka Komiyama
Satoshi Yamazaki
Hisako Katagiri
Chisato Shimazu
Sumiyoshi Naito
Masato Matsuda
Yoichi Yuki
Kazuyoshi Miyata
Rika Arai
Osamu Kumano
Mari Emmi
Tatsuya Yoshida
Hideaki Tanaka
Toshihiko Okuhara
Tetsuji Yamashita
Yasuji Sekine
Masaru Homma
Yohko Kawai
Kaoru Tohyama
The working group for standardization of sample preparation for clotting time assays, Japanese society for laboratory hematology.
Publikationsdatum
14.09.2020
Verlag
Springer Singapore
Erschienen in
International Journal of Hematology / Ausgabe 5/2020
Print ISSN: 0925-5710
Elektronische ISSN: 1865-3774
DOI
https://doi.org/10.1007/s12185-020-02983-x

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