Exploring the potential of Moringa oleifera seed extract-loaded microemulsion hydrogel in the DNCB-induced atopic dermatitis model
- 10.05.2025
- Original Article
Erschienen in:
- Verfasst von
- Prakrati Garg
- Aaliya Ali
- Sewa Singh
- Kanika Thakur
- Kaisar Raza
- Bigul Yogeshver Bhardwaj
- Saurabh Kulshrestha
- Poonam Negi
- Erschienen in
- Inflammopharmacology | Ausgabe 8/2025
Abstract
Background
Moringa oleifera n-hexane (MO n-Hex) seed extract contains phytoconstituents such as behenic acid, quercetin, and kaempferol. These exhibit anti-inflammatory, antiallergic, and antioxidant properties and can alleviate atopic dermatitis (AD)-like symptoms.
Purpose
This study aimed to develop and evaluate MO n-Hex seed extract-loaded microemulsion (ME) hydrogel to provide an effective, safe, non-steroidal, plant-based alternative to conventional therapies in the topical management of AD.
Methods
Optimized o/w ME was developed by building pseudo-ternary phase diagrams. Ex vivo skin penetration was determined by employing CLSM analysis. Further, skin compatibility, histological analysis, and pharmacodynamics were carried out using a DNCB-induced AD model in BALB/c mice.
Results
The best o/w ME demonstrated nearly spherical globules with size < 50 nm, zeta potential − 28.83 ± 0.492, and pH value 5.433 ± 0.047. The in vivo efficacy revealed significant improvements in AD-like symptoms, healed ear skin lesions, and lowered IgE levels and inflammatory cytokiness (IL-4, IL-5, and IFN-γ). Further, histological analysis confirmed the restoration of skin structure, supporting the formulation’s potential in skin barrier repair.
Conclusions
The study demonstrated that the MO n-Hex seed extract-loaded MEs were suitable for topical use with improved penetration to deeper layers of skin while showing safety and better skin compliance. The formulated MEs effectively modulated immune responses and restored skin structure and barrier functioning.
Graphical abstract
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- Titel
- Exploring the potential of Moringa oleifera seed extract-loaded microemulsion hydrogel in the DNCB-induced atopic dermatitis model
- Verfasst von
-
Prakrati Garg
Aaliya Ali
Sewa Singh
Kanika Thakur
Kaisar Raza
Bigul Yogeshver Bhardwaj
Saurabh Kulshrestha
Poonam Negi
- Publikationsdatum
- 10.05.2025
- Verlag
- Springer International Publishing
- Erschienen in
-
Inflammopharmacology / Ausgabe 8/2025
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608 - DOI
- https://doi.org/10.1007/s10787-025-01740-y
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