Skip to main content
main-content

01.12.2014 | Research | Ausgabe 1/2014 Open Access

Journal of Ovarian Research 1/2014

Expression and activity of Rac1 is negatively affected in the dehydroepiandrosterone induced polycystic ovary of mouse

Zeitschrift:
Journal of Ovarian Research > Ausgabe 1/2014
Autoren:
Vineet Kumar Maurya, Chadchan Sangappa, Vijay Kumar, Sahil Mahfooz, Archana Singh, Singh Rajender, Rajesh Kumar Jha
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1757-2215-7-32) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

VKM carried out PCOS model preparation and development of Rac1, pRac1, Vav and pVav immuno-blot. SC performed Western blotting of Caveolin1, Rac1, pRac1, Vav and pVav along with the 17β-estradiol assay. VK performed the Rac1 activity assay, tissue paraffin embedding and microtomy. SM did the histological examination of ovarian tissue sections and helped in manuscript drafting. AS performed the immuno-precipitation of Vav, immuno-blotting with Rac1 and Vav along with the inhibin B assay. RS assisted in drafting the manuscript and experimental design. RKJ designed, analyzed the data and finalized the manuscript. All authors read and approved the final manuscript.

Abstract

Background

Polycystic ovarian syndrome (PCOS) is characterized by the presence of multiple follicular cysts, giving rise to infertility due to anovulation. This syndrome affects about 10% of women, worldwide. The exact molecular mechanism leading to PCOS remains obscure. RhoGTPase has been associated with oogenesis, but its role in PCOS remains unexplored. Therefore, we attempted to elucidate the Vav-Rac1 signaling in PCOS mice model.

Methods

We generated a PCOS mice model by injecting dehydroepiandrosterone (DHEA) for a period of 20 days. The expression levels of Rac1, pRac1, Vav, pVav and Caveolin1 were analyzed by employing immuno-blotting and densitometry. The association between Vav and Rac1 proteins were studied by immuno-precipitation. Furthermore, we analyzed the activity of Rac1 and levels of inhibin B and 17β-estradiol in ovary using biochemical assays.

Results

The presence of multiple follicular cysts in ovary were confirmed by histology. The activity of Rac1 (GTP bound state) was significantly reduced in the PCOS ovary. Similarly, the expression levels of Rac1 and its phosphorylated form (pRac1) were decreased in PCOS in comparison to the sham ovary. The expression level and activity (phosphorylated form) of guanine nucleotide exchanger of Rac1, Vav, was moderately down-regulated. We observed comparatively increased expressions of Caveolin1, 17β-estradiol, and inhibin B in the polycystic ovary.

Conclusion

We conclude that hyperandrogenization (PCOS) by DHEA diminishes ovarian Rac1 and Vav expression and activity along with an increase in expression of Caveolin1. This is accompanied by an increase in the intra-ovarian level of '17 β-estradiol and inhibin B.
Zusatzmaterial
Authors’ original file for figure 1
13048_2013_319_MOESM1_ESM.tiff
Authors’ original file for figure 2
13048_2013_319_MOESM2_ESM.tiff
Authors’ original file for figure 3
13048_2013_319_MOESM3_ESM.tiff
Authors’ original file for figure 4
13048_2013_319_MOESM4_ESM.tiff
Authors’ original file for figure 5
13048_2013_319_MOESM5_ESM.tiff
Authors’ original file for figure 6
13048_2013_319_MOESM6_ESM.tiff
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 1/2014

Journal of Ovarian Research 1/2014 Zur Ausgabe


 

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Meistgelesene Bücher aus dem Fachgebiet

2014 | Buch

Perikonzeptionelle Frauenheilkunde

Fertilitätserhalt, Prävention und Management von Schwangerschaftsrisiken

Ein Praxisbuch für alle, die in der Beratung und Betreuung von Schwangeren und Paaren mit Kinderwunsch tätig sind. Die Herausgeber und Autoren haben alle wichtigen Informationen zusammengetragen, um Frauen sicher zu beraten, zu betreuen und kompetent Verantwortung zu übernehmen.

Herausgeber:
Christian Gnoth, Peter Mallmann

2019 | Buch

Praxisbuch Gynäkologische Onkologie

Dieses kompakte und praxisrelevante Standardwerk richtet sich alle Ärzte in Klinik und Praxis, die Patientinnen mit bösartigen Tumoren des Genitales und der Mamma behandeln. Die 5. Auflage wurde komplett aktualisiert und auf der Basis …

Herausgeber:
Prof. Dr. Edgar Petru, Dr. Daniel Fink, Prof. Dr. Ossi R. Köchli, Prof. Dr. Sibylle Loibl

Mail Icon II Newsletter

Bestellen Sie unseren kostenlosen Newsletter Update Gynäkologie und bleiben Sie gut informiert – ganz bequem per eMail.

Bildnachweise