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01.01.2004 | Original Article

Expression of fibronectin splice variants and oncofetal glycosylated fibronectin in the synovial membranes of patients with rheumatoid arthritis and osteoarthritis

verfasst von: J. Kriegsmann, A. Berndt, T. Hansen, L. Borsi, L. Zardi, R. Bräuer, P. K. Petrow, M. Otto, C. J. Kirkpatrick, S. Gay, H. Kosmehl

Erschienen in: Rheumatology International | Ausgabe 1/2004

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Abstract

Objective

The aim of this study was to define and compare the expression of fibronectin (Fn) isoforms in synovial tissue of patients with rheumatoid arthritis (RA) and osteoarthritis (OA).

Methods

Using monoclonal antibodies specific for total Fn, extra domain (ED)-A Fn, ED-B Fn, and oncofetal glycosylated Fn, we studied the expression of the Fn isoforms in synovium. Furthermore, in situ hybridization for the detection of ED-B Fn mRNA including a double labeling technique for the detection of cell type was applied.

Results

Strong expression of total Fn, ED-A Fn, oncofetal glycosylated Fn and, to a lesser extent, ED-B Fn could be demonstrated in the synovial lining layer in both RA and OA. Stromal and vessel expression of Fn isoforms was more prominent in RA tissue. Pannus tissue showed strong labeling with ED-B Fn.

Conclusion

The expression of alternatively spliced isoforms of Fn is associated with tissue remodeling and, as a partial process of this phenomenon, with neovascularization rather than underlying disease, X-ray status, or parameters of acute inflammation. In the lining layer, Fn expression correlates with hyperplasia associated with cell recruitment but not with proliferative status. Most remarkably, the expression of ED-B Fn in pannus tissue seems to be associated with the invasive phenotype described in RA tissue.

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Titel: Expression of fibronectin splice variants and oncofetal glycosylated fibronectin in the synovial membranes of patients with rheumatoid arthritis and osteoarthritis
verfasst von: J. Kriegsmann
A. Berndt
T. Hansen
L. Borsi
L. Zardi
R. Bräuer
P. K. Petrow
M. Otto
C. J. Kirkpatrick
S. Gay
H. Kosmehl
Publikationsdatum: 01.01.2004
Verlag: Springer-Verlag
Erschienen in: Rheumatology International / Ausgabe 1/2004
Print ISSN: 0172-8172
Elektronische ISSN: 1437-160X
DOI: https://doi.org/10.1007/s00296-003-0316-1

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Expression of fibronectin splice variants and oncofetal glycosylated fibronectin in the synovial membranes of patients with rheumatoid arthritis and osteoarthritis

Abstract

Objective

Methods

Results

Conclusion

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Abstract

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Methods

Results

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