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01.12.2012 | Research | Ausgabe 1/2012 Open Access

Reproductive Biology and Endocrinology 1/2012

Expression of HOXA11 in the mid-luteal endometrium from women with endometriosis-associated infertility

Zeitschrift:
Reproductive Biology and Endocrinology > Ausgabe 1/2012
Autoren:
Malgorzata Szczepańska, Przemyslaw Wirstlein, Jana Skrzypczak, Paweł P Jagodziński
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1477-7827-10-1) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

MS contributed to designing the study, acquisition of data, analysis and interpretation of data, and in writing the manuscript. PW participated in the in the acquisition and interpretation of the data. As Principal Investigators, JS and JPP were involved in the intellectual and experimental programming of the study, the assays and interpretation of data, and writing the manuscript. All authors read and approved the final manuscript.

Abstract

Background

A decrease in HOXA11 expression in eutopic mid-secretory endometrium has been found in women with endometriosis-associated infertility.

Methods

Using Real-time quantitative PCR (RQ-PCR) and western blotting analysis we studied the HOXA11 transcript and protein levels in mid-luteal eutopic endometrium from eighteen infertile women with minimal endometriosis, sixteen healthy fertile women and sixteen infertile women with fallopian tubal occlusion from the Polish population. We also evaluated transcript levels of DNA methyltransferases DNMT1, DNMT3A and DNMT3B in these groups of women.

Results

There were significantly lower levels of HOXA11 transcripts (p = 0.003, p = 0.041) and protein (p = 0.004, p = 0.001) in women with endometriosis as compared to fertile women and infertile women with tubal occlusion. Moreover, we found significantly higher methylation levels of the CpG region in the first exon of HOXA11 in infertile women with endometriosis compared with fertile women (p < 0.001) and infertile women with tubal occlusion (p < 0.001). We also observed significantly increased levels of DNMT3A transcript in women with endometriosis than fertile women (p = 0.044) and infertile women with tubal occlusion (p = 0.047). However, we did not observe significant differences in DNMT1 and DNMT3B transcript levels between these investigated groups of women.

Conclusions

We confirmed that reduced HOXA11 expression may contribute to endometriosis-associated infertility. Moreover, we found that DNA hypermethylation can be one of the possible molecular mechanisms causing a decrease in HOXA11 expression in the eutopic mid-secretory endometrium in infertile women with endometriosis.
Zusatzmaterial
Additional file 1: Supplemental Table S1. Primer sequences used for RQ-PCR analysis and bisulfite sequencing of HOXA11 regions I, II, and III. (PDF 14 KB)
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Additional file 2: Supplemental Figure S1. Location of CpG-rich regions I, II, and III in the human HOXA11 gene. (PDF 66 KB)
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Authors’ original file for figure 1
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Authors’ original file for figure 2
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Authors’ original file for figure 3
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Literatur
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