Expression of matrix metalloproteinase-9 (MMP-9) in human skin within 1 hour after injury through immunohistochemical staining: a pilot study
- 01.05.2024
- Original Article
Erschienen in:
- Verfasst von
- Rossana Cecchi
- Tomoya Ikeda
- Jessika Camatti
- Mizuho Nosaka
- Yuko Ishida
- Toshikazu Kondo
- Erschienen in
- International Journal of Legal Medicine | Ausgabe 5/2024
Abstract
Matrix metalloproteinase-9 (MMP-9) is involved in tissue remodeling and in skin wound healing. The present study focuses on the MMP-9 expression in epidermal wound healing within 1 h after injury, to test whether MMP-9 can be used to estimate the time of injury in forensic practice.
A sample consisting of 5 individuals undergoing surgery was analyzed. With the consent of the patients, sections of skin were removed from the surgical wound at predefined time intervals. For each subject, 8 sections were taken, one for each time interval defined at 0 ‘- 1’ − 3 ‘- 5’ − 10 ‘- 15’ − 30 ‘- 60’ minutes. The specimens were immunostained with MMP-9, and the number of positively stained cells was examined.
The number of positively stained cells showed an increasing trend as a function of time. Less than 30 positively stained cells were found in all cases within 3 min. At the post-infliction time of 5 min, the number of positively stained cells exceeded 30 in 3 out of 5 cases. The number of MMP-positive cells exceeded 40 in all cases in over 10 min.
In the light of these results, the count of MMP-9 positive cells might be a useful marker in the wound-age estimation within 1 h in forensic setting. More research is required to collect more samples and to compare samples from the hyperacute phase with those from several days after injury.
Anzeige
- Titel
- Expression of matrix metalloproteinase-9 (MMP-9) in human skin within 1 hour after injury through immunohistochemical staining: a pilot study
- Verfasst von
-
Rossana Cecchi
Tomoya Ikeda
Jessika Camatti
Mizuho Nosaka
Yuko Ishida
Toshikazu Kondo
- Publikationsdatum
- 01.05.2024
- Verlag
- Springer Berlin Heidelberg
- Erschienen in
-
International Journal of Legal Medicine / Ausgabe 5/2024
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596 - DOI
- https://doi.org/10.1007/s00414-024-03243-x
Dieser Inhalt ist nur sichtbar, wenn du eingeloggt bist und die entsprechende Berechtigung hast.
