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Child's Nervous System
Erschienen in: Child's Nervous System 12/2017

16.08.2017 | Original Paper

Expression of microRNAs in tumors of the central nervous system in pediatric patients in México

Erschienen in: Child's Nervous System | Ausgabe 12/2017

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Abstract

Purpose

MicroRNAs were identified as molecules that participate in gene regulation; alterations in their expression characterize central nervous system (CNS). Information in pediatrics is scarce, so the objective of this work was to determine and then compare the patterns of expression of microRNAs in astrocytomas, ependymomas, and medulloblastomas, as well as in non-neoplastic brain.

Methods

Low-density arrays were utilized to evaluate 756 microRNAs in three samples of each type of tumor and non-neoplastic brain. The relative expression was calculated in order to identify the three microRNAs whose expression was modified notably. This was verified using RT-qPCR in more number of tumor samples.

Results

The microRNAs selected for testing were miR-100-5p, miR-195-5p, and miR-770-5p. A higher expression of miR-100-5p was observed in the astrocytomas and ependymomas compared to the medulloblastomas: on average 3.8 times (p < 0.05). MiR-770-5p was expressed less in medulloblastomas compared to astrocytomas four times (p = 0.0162). MiR-195-5p had a low expression in medulloblastomas compared to non-neoplastic cerebellum (p = 0.049). In all three tumor types, expression of miR-770-5p was lower than in non-neoplastic brain (p < 0.001).

Conclusions

These microRNAs may represent potential markers in these tumors.
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Metadaten
Titel
Expression of microRNAs in tumors of the central nervous system in pediatric patients in México
Publikationsdatum
16.08.2017
Erschienen in
Child's Nervous System / Ausgabe 12/2017
Print ISSN: 0256-7040
Elektronische ISSN: 1433-0350
DOI
https://doi.org/10.1007/s00381-017-3569-9

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