Background
Methods
Patients and specimens
Real-time PCR (RT-PCR)
Immunohistochemistry staining and evaluation
Statistical analysis
Results
LMVD counting
Clinicopathological parameters | N | ECM1 expression | LMVD | |||
---|---|---|---|---|---|---|
- | + |
P
| (LMV per × 200 field) |
P
| ||
Gender
| 0.618 | 0.627 | ||||
Male | 47 | 13 | 34 | 7.76 ± 1.21 | ||
Female | 30 | 10 | 20 | 7.83 ± 1.23 | ||
Age (years)
| 0.628 | 0.553 | ||||
<60 | 37 | 10 | 27 | 7.25 ± 1.17 | ||
≥60 | 40 | 13 | 27 | 8.34 ± 1.33 | ||
Differentiation degree
| 0.489 | 0.253 | ||||
Low | 36 | 11 | 25 | 9.25 ± 1.37 | ||
Moderate | 23 | 5 | 18 | 5.75 ± 1.22 | ||
Well | 18 | 7 | 11 | 7.94 ± 2.01 | ||
Depth of invasion
| <0.0001*** | 0.721 | ||||
T1-T2 | 23 | 18 | 5 | 8.77 ± 1.97 | ||
T3-T4 | 54 | 5 | 49 | 7.53 ± 0.99 | ||
Lymph node metastasis
| 0.521 | 0.110 | ||||
0 | 34 | 9 | 25 | 6.74 ± 1.29 | ||
1-2 | 18 | 9 | 11 | 7.00 ± 2.46 | ||
3-6 | 10 | 3 | 7 | 11.00 ± 2.74 | ||
≥7 | 15 | 4 | 11 | 9.57 ± 1.47 | ||
TNM stage
| 0.0014** | 0.241 | ||||
0-I | 21 | 12 | 9 | 8.50 ± 2.12 | ||
II-III | 27 | 11 | 45 | 8.67 ± 1.18 |
ECM1 expression
Tissue type | mRNA level of ECM1 | F | Positive rate (%) | Staining grades of ECM1 |
χ
2
| |||
---|---|---|---|---|---|---|---|---|
- | + | ++ | +++ | |||||
Tumor specimen | 0.010 ± 0.002 | 70.1(54/77) | 23 | 35 | 13 | 6 | ||
Non-cancerous mucosa | 0.007 ± 0.002 | 7.48** | 7.8(6/77) | 71 | 5 | 1 | 0 | 62.91*** |
ECM1 expression and LMVD
Tissue type | ECM1 immunostaining | Spearman R |
P
| |
---|---|---|---|---|
- | + | |||
Tumor specimen | (23/77) 4.47 ± 0.95 | (54/77) 9.22 ± 1.13 | 0.407 | 0.001** |
Non-cancerous mucosa | (71/77) 2.71 ± 0.41 | (6/77) 2.00 ± 1.05 | -0.061 | 0.633 |