Skip to main content
Erschienen in: Journal of the International AIDS Society 4/2010

Open Access 01.11.2010 | Poster presentation

Feasibility of testing antibodies to HIV from filter paper using HIV rapid test kits

verfasst von: A Ganesan, G Thatchinamoorthy, S Saramini

Erschienen in: Journal of the International AIDS Society | Sonderheft 4/2010

download
DOWNLOAD
print
DRUCKEN
insite
SUCHEN
HIV rapid tests are widely used in resource limited settings. Dried blood spots on filter paper are an easy way to collect and transport blood samples from a remote area to a testing facility at ambient temperatures. Our objective was to evaluate the technical possibility of testing antibodies to HIV from filter paper using HIV rapid tests kits.
Dried blood spots on filter paper were obtained from 408 female sex workers (FSW), 136 Men Sex with Men (MSM) and 50 Intravenous Drug Users (IDU). Antibodies were eluted from DBS disks (punch 6 mm, approximately 5 µl serum) in 200 µl of sterile Phosphate Buffer Saline (PBS) (0.01 M, with 0.1% sodium azide) by agitating overnight at 2-8° C (refrigerator) for minimum 16 hours and tested for anti-HIV antibody using two ELISA (Microlisa, Enzaids). As per the guidelines of the National AIDS Control Organization Sentinel Surveillance of HIV, the reactive samples on the first ELISA are retested using a 2nd ELISA kit. We assessed all the HIV positive and HIV negative DBS samples using 3 HIV rapid tests kits (Combaids, Determine and EIAComb) procedures were followed according to the manufacturer's instructions.
Of the 594 DBS samples, 22 (3.69%) samples were positive for HIV antibodies using ELISA kits. Among HIV positive DBS samples, 3.67% (15/408) were FSW's, 2.95% (4/136) were MSM and 6% (3/50) were IDU's. All 22 DBS were positive in both ELISA kits (Microlisa, Enzaids) and the sensitivity and specificity of both kits were 100%, whereas Determine found 20 HIV positives, Combaids 21 samples Positive and EIAComb found all 22 HIV positive giving sensitivity was 90.91%, 95.45%, and 100% respectively and specificity was 100%.
This study demonstrates and confirms the usefulness and feasibility of the filter paper blood collection method for testing of HIV antibodies. DBS can be used with HIV rapid test devices. Using the latest and advanced techniques applied in rapid test technologies, DBS may provide a unique way to conduct sero epidemiological surveys in resource limited settings.
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
download
DOWNLOAD
print
DRUCKEN
Metadaten
Titel
Feasibility of testing antibodies to HIV from filter paper using HIV rapid test kits
verfasst von
A Ganesan
G Thatchinamoorthy
S Saramini
Publikationsdatum
01.11.2010
Verlag
BioMed Central
Erschienen in
Journal of the International AIDS Society / Ausgabe Sonderheft 4/2010
Elektronische ISSN: 1758-2652
DOI
https://doi.org/10.1186/1758-2652-13-S4-P147

Weitere Artikel der Sonderheft 4/2010

Journal of the International AIDS Society 4/2010 Zur Ausgabe

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.