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European Journal of Nutrition

Erschienen in:

05.01.2016 | Original Contribution

Fenugreek increases insulin-stimulated creatine content in L6C11 muscle myotubes

verfasst von: Kristyen A. Tomcik, William J. Smiles, Donny M. Camera, Helmut M. Hügel, John A. Hawley, Rani Watts

Erschienen in: European Journal of Nutrition | Ausgabe 3/2017

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Abstract

Purpose

Creatine uptake by muscle cells is increased in the presence of insulin. Accordingly, compounds with insulin-like actions may also augment creatine uptake. The aim of this study was to investigate whether Trigonella foenum-graecum (fenugreek), an insulin mimetic, increases total intracellular creatine levels in vitro.

Methods

Total cellular creatine content was measured fluorometrically in L6C11 muscle myotubes treated for 1, 4, and 24 h with 0.5 mM creatine (CR), CR and 20 μg/mL fenugreek seed extract (CR + FEN), CR and 100 nM insulin (CR + INS), and CR + INS + FEN (n = 6 per treatment group). Alterations in the expression of the sodium- and chloride-dependent creatine transporter, SLC6A8, and key signaling proteins in the PI3-K/Akt pathway were determined.

Results

Compared to control (CON), CR + INS + FEN increased total creatine content after 4 h (P < 0.05), whereas all conditions increased SLC6A8 protein expression above CON at this time (P < 0.05). Changes in insulin signaling were demonstrated via increases in Akt^Thr308 phosphorylation, with CR + INS > CON and CR at 1 h (P < 0.05) and with CR + INS + FEN > CON, CR, and CR + INS at 4 h (P < 0.05). In contrast, no changes in PKCζ/λ or GLUT4 phosphorylation were detected.

Conclusion

Fenugreek, when combined with insulin, modulates creatine content via a mechanism which is independent of the activity of SLC6A8, suggesting that an alternative mechanism is responsible for the regulation and facilitation of insulin-mediated creatine uptake in skeletal muscle cells.

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Titel: Fenugreek increases insulin-stimulated creatine content in L6C11 muscle myotubes
verfasst von: Kristyen A. Tomcik
William J. Smiles
Donny M. Camera
Helmut M. Hügel
John A. Hawley
Rani Watts
Publikationsdatum: 05.01.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 3/2017
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-015-1145-1

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Fenugreek increases insulin-stimulated creatine content in L6C11 muscle myotubes

Abstract

Purpose

Methods

Results

Conclusion

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Abstract

Purpose

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