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Medical Molecular Morphology
Erschienen in:

03.04.2023 | Original Paper

FGF4 and FGF9 have synergistic effects on odontoblast differentiation

verfasst von: Tatsuki Hoshino, Shoko Onodera, Motoyoshi Kimura, Makoto Suematsu, Tatsuya Ichinohe, Toshifumi Azuma

Erschienen in: Medical Molecular Morphology | Ausgabe 3/2023

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Abstract

The purpose of this study was to investigate whether fibroblast growth factor 4 (FGF4) and FGF9 are active in dentin differentiation. Dentin matrix protein 1 (Dmp1) -2A-Cre transgenic mice, which express the Cre-recombinase in Dmp1-expressing cells, were crossed with CAG-tdTomato mice as reporter mouse. The cell proliferation and tdTomato expressions were observed. The mesenchymal cell separated from neonatal molar tooth germ were cultured with or without FGF4, FGF9, and with or without their inhibitors ferulic acid and infigratinib (BGJ398) for 21 days. Their phenotypes were evaluated by cell count, flow cytometry, and real-time PCR. Immunohistochemistry for FGFR1, 2, and 3 expression and the expression of DMP1 were performed. FGF4 treatment of mesenchymal cells obtained promoted the expression of all odontoblast markers. FGF9 failed to enhance dentin sialophosphoprotein (Dspp) expression levels. Runt-related transcription factor 2 (Runx2) was upregulated until day 14 but was downregulated on day 21. Compared to Dmp1-negative cells, Dmp1-positive cells expressed higher levels of all odontoblast markers, except for Runx2. Simultaneous treatment with FGF4 and FGF9 had a synergistic effect on odontoblast differentiation, suggesting that they may play a role in odontoblast maturation.
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Metadaten
Titel
FGF4 and FGF9 have synergistic effects on odontoblast differentiation
verfasst von
Tatsuki Hoshino
Shoko Onodera
Motoyoshi Kimura
Makoto Suematsu
Tatsuya Ichinohe
Toshifumi Azuma
Publikationsdatum
03.04.2023
Verlag
Springer Nature Singapore
Erschienen in
Medical Molecular Morphology / Ausgabe 3/2023
Print ISSN: 1860-1480
Elektronische ISSN: 1860-1499
DOI
https://doi.org/10.1007/s00795-023-00351-2

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