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01.12.2016 | Research | Ausgabe 1/2016 Open Access

Annals of Intensive Care 1/2016

Fluorescence imaging of ATP in neutrophils from patients with sepsis using organelle-localizable fluorescent chemosensors

Zeitschrift:
Annals of Intensive Care > Ausgabe 1/2016
Autoren:
Koichiro Sueyoshi, Yuka Sumi, Yoshiaki Inoue, Yoko Kuroda, Kumiko Ishii, Hitoshi Nakayama, Kazuhisa Iwabuchi, Yasutaka Kurishita, Hajime Shigemitsu, Itaru Hamachi, Hiroshi Tanaka
Abbreviations
ANOVA
analysis of variance
APACHE
Acute Physiology and Chronic Health Evaluation
CLP
cecal ligation and puncture
CRP
C-reactive protein
DIC
disseminated intravascular coagulation
FACS
fluorescence-activated cell sorting
fMLP
N-formyl-met-leu-phe
HBSS
Hanks’ balanced salt solution
HC
healthy controls
JAAM
Japanese Association for Acute Medicine
MFI
mean fluorescence intensity
PMN
polymorphonuclear neutrophil
SOFA
Sequential Organ Failure Assessment
WBC
white blood cell

Background

ATP is well known as the energy currency of cells and is involved in both intracellular signaling and extracellular signaling. Although polymorphonuclear neutrophils (PMNs) are one of the most important phagocytic cell types in defending against invading pathogens and play a pivotal role in inflammation, their activation can be a double-edged sword. In particular, their pathophysiological processes can damage the host tissue when activated during sepsis, trauma, ischemia–reperfusion injury, and inflammatory diseases [1]. PMNs can migrate to compromised tissues in response to chemotactic gradients. The activation of PMNs is triggered by the recognition of bacterial peptides and innate inflammatory mediators. Although a signal amplification system is crucial for the activation of PMNs, the underlying mechanisms remain obscure [24]. We previously found that ATP was released extracellularly by PMNs through pannexin-1 (PANX1) hemichannels and that autocrine feedback through the P2Y2 receptor, one of eight P2Y G protein-coupled receptors, is an essential mechanism for activating PMNs [5, 6]. We also reported that plasma ATP levels were correlated with PMN activation and were significantly higher in mouse models of cecal ligation and puncture (CLP) [7].
In recent decades, much effort has been dedicated to developing fluorescent chemosensors for ATP to study its diverse functions [8, 9]. Consequently, two new fluorescent chemosensors were developed (PMAP-1 and MitoAP-1), which can detect ATP and its derivatives in specific regions of living cells [10]. PMAP-1 and MitoAP-1 are also referred to as 2-2Zn(ΙΙ) and 3-2Zn(ΙΙ), respectively [10]. PMAP-1 can localize to the plasma membrane and detect the extracellular release of ATP. In contrast, MitoAP-1 can spontaneously localize in the mitochondria and detect local ATP.
Sepsis is the major cause of death in critically ill patients and is the predominant cause of multiple organ dysfunction syndrome [11, 12]. In spite of progress in intensive care medicine and strict adherence to established treatment protocols such as the “Surviving Sepsis Campaign” guidelines [13], mortality in sepsis patients remains high. Millions of patients worldwide are afflicted each year, resulting in the death of one in three patients [11]. Multiple organ failure in sepsis is the prevailing cause of death, and mitochondrial dysfunction has been suggested to contribute to the development of organ dysfunction and failure in sepsis [14]. However, the associated pathogenic mechanisms remain unclear.
In this study, we evaluated a new approach to quantify ATP release on the cell surface and ATP production in the mitochondria of PMNs by flow cytometry. As described above, ATP is known to play a key role in the activation of PMNs; thus, we hypothesized that ATP levels on the plasma membrane and in the mitochondria of PMNs could serve as an informative biomarker for sepsis. Here, we report the correlation between ATP levels and the clinical conditions of sepsis patients.

Methods

Patients

After obtaining approval from the ethics committee of Juntendo University, Urayasu Hospital (25–47), 16 sepsis patients admitted to our critical care center between October 2013 and April 2015 were enrolled in the study. All patients met the diagnostic criteria for sepsis determined by the American College of Chest Physicians and Society of Critical Care Medicine [15], and all patients (or their legal representatives) provided informed consent for participation in the research. Patients with malignant disease or chronic administration of steroids were excluded. The patients comprised nine males and seven females, with a mean ± SD age of 67 ± 9 years, a serum C-reactive protein (CRP) level of 27.0 ± 12.1 mg/dL, a white blood cell (WBC) count of 13.3 ± 9.1 × 103/μL, an Acute Physiology and Chronic Health Evaluation (APACHE) II score [16] of 22.1 ± 7.8, a Sequential Organ Failure Assessment (SOFA) score [17] of 7.9 ± 4.6, and a Japanese Association for Acute Medicine (JAAM) disseminated intravascular coagulation (DIC) score [18] of 3.8 ± 1.7. The cause of sepsis was urinary tract infection in five patients; burn in three patients; pneumonia in three patients; and necrotic cholecystitis, cellulitis, gas gangrene, cervical abscess, and peritonitis in one patient each. Two patients died during hospitalization (Table 1). The healthy control (HC) group consisted of six males and three females, with a mean ± SD age of 37 ± 5 years.
Table 1
Characteristics of sepsis patients
Patients
Cause of sepsis
APACHE ΙΙ score
SOFA score
JAAM DIC score
WBC (/μL)
CRP (mg/dL)
Mortality
1.
Necrotic cholecystitis
31
15
7
4600
27.9
Alive
2.
Pneumonia
18
3
4
18,600
48.8
Alive
3.
Burn
12
2
2
10,600
23.9
Alive
4.
Pneumonia
24
7
3
17,900
35.8
Dead
5.
Cellulitis
11
3
3
19,300
54.0
Alive
6.
Burn
29
12
4
4100
14.2
Dead
7.
UTI
36
14
8
13,900
17.9
Alive
8.
UTI
26
11
3
16,400
15.5
Alive
9.
UTI
14
6
3
20,700
10.4
Alive
10.
Burn
29
13
4
4600
25.5
Alive
11.
UTI
20
8
6
31,900
25.8
Alive
12.
Gas gangrene
17
2
2
17,100
15.6
Alive
13.
Cervical abscess
20
5
3
5000
29.4
Alive
14.
UTI
11
4
3
800
29.7
Alive
15.
Peritonitis
29
9
3
23,700
21.8
Alive
16.
Pneumonia
27
13
3
900
36.0
Alive
APACHE Acute Physiology and Chronic Health Evaluation, SOFA Sequential Organ Failure Assessment, JAAM Japanese Association for Acute Medicine, DIC disseminated intravascular coagulation, WBC white blood cell counts, CRP C-reactive protein, UTI urinary tract infection

Blood samples

A 10-mL heparinized blood sample was collected via the peripheral vein of each healthy volunteer and via arterial lines in sepsis patients. Blood collection for all sepsis patients was performed within 24 h after the patients were diagnosed with sepsis (days 0–1) and at days 3–4 of sepsis.

Isolation of PMNs

Human PMNs were isolated from the heparinized whole blood of healthy volunteers and sepsis patients using Polymorphprep™ centrifugation (Alere Technologies AS, Oslo, Norway). The neutrophil population was >95 % pure, as determined by Wright–Giemsa staining.

PMAP-1 and MitoAP-1

Both PMAP-1 and MitoAP-1 were designed on the basis of a fluorescent ATP probe containing a xanthene fluorophore [10]. PMAP-1 selectively localizes on the plasma membrane via a biocompatible anchor for the membrane, which possesses a hydrophobic oleyl moiety at the end of a long ethylene glycol linker [10]. MitoAP-1 selectively localizes to the mitochondria via a positively charged pyronin ring [10].

Imaging of PMNs with confocal microscopy

Isolated human PMNs (2.0 × 106 cells/mL) were plated on a 35-mm glass-bottomed dish (IWAKI, Tokyo, Japan), coated with 100 μL of poly-l-lysine solution (Sigma-Aldrich, St. Louis, MO, USA), and incubated for 10 min. All incubations were performed under a humidified atmosphere of 5 % CO2 at 37 °C in the dark. Cells were treated with 400 nM (final concentration) of MitoTracker® Green FM (Invitrogen, Carlsbad, CA, USA), followed by incubation with 1 μM MitoAP-1 (final concentration) for 20 min. Cells were incubated with 5 μM PMAP-1 (final concentration) for 10 min. After washing twice with 37 °C Hanks’ balanced salt solution (HBSS, Sigma-Aldrich), stained cells were examined with a TCS SP5 Leica confocal laser-scanning microscope equipped with a Plan-Apochromat 100× oil-immersion objective lens at 37 °C, under a 5 % CO2 humidified atmosphere.

Fluorescence quantification by flow cytometry

Isolated PMNs were washed with HBSS and incubated at 37 °C for 10 min with or without N-formyl-met-leu-phe (fMLP, ScyTek Laboratories, Logan, UT, USA) stimulation before staining. Cells were incubated with 1 μM (final concentration) of MitoAP-1 and allophycocyanin-conjugated anti-CD11b antibodies (BD Biosciences, San Jose, CA, USA) at 37 °C for 20 min, followed by incubation with 5 μM PMAP-1 (final concentration) at 37 °C for 10 min. The cells were placed on ice for 10 min and centrifuged at 1500 rpm for 5 min at 4 °C. After washing twice with HBSS, cells were fixed with fluorescence-activated cell sorting (FACS) sheath fluid (BD Biosciences) and then analyzed by flow cytometry (FACSCalibur, BD Bioscience).

Statistical analysis

The data are expressed as the mean ± SEM unless stated otherwise. Statistical analyses were performed using GraphPad Prism version 6.0 (GraphPad, San Diego, CA, USA). The data were analyzed for significant differences by a paired t test or Wilcoxon signed-rank test for comparisons of paired groups. One-way analysis of variance (ANOVA) and the Kruskal–Wallis test were used for comparisons of three groups. Spearman’s rank correlation was performed as indicated. Differences were considered statistically significant at p < 0.05.

Results

Successful establishment of a staining protocol for human PMNs with MitoAP-1

Imaging studies using PMAP-1 and MitoAP-1 were limited to cultured cells [10] and T cells [19] until Bao et al. [20] first reported imaging studies of human PMNs using PMAP-1. As in their study, the bright green fluorescence emitted by PMAP-1 was predominantly observed on the plasma membrane of PMNs (Fig. 1a). However, there has been no previous report of the successful staining of human PMNs with MitoAP-1. We observed a punctate pattern of bright red fluorescence emitted by MitoAP-1 within the cells. MitoAP-1 fluorescence overlapped well with that of MitoTracker® Green FM, a typical dye used to stain the mitochondria, indicating that MitoAP-1 selectively and spontaneously localized to the mitochondria (Fig. 1b). Thus, we successfully established a method for staining human PMNs with MitoAP-1.

ATP release on the plasma membrane of HC PMNs increased with fMLP stimulation

Stimulation of the formyl peptide receptor was previously found to cause ATP release from PMNs through maxi-anion channels and PANX1 hemichannels [6]. To confirm this finding, the MFI of PMAP-1 on the plasma membrane of HC PMNs was quantified by flow cytometry after stimulation with the indicated concentrations of fMLP (Fig. 2a). The MFI of PMAP-1 following fMLP stimulation increased in a dose-dependent manner. CD11b expression on the plasma membrane of PMNs was also measured as a marker of PMN activation. Cell surface CD11b expression in PMNs stimulated with fMLP also increased in a dose-dependent manner (Fig. 2b).

Mitochondrial ATP levels in the HC group decreased with fMLP stimulation

Mitochondria are often referred to as the powerhouse of the cells, as they generate ATP by oxidative phosphorylation. To our knowledge, changes in mitochondrial ATP levels in PMNs have not been reported. The MFI of MitoAP-1 following fMLP stimulation decreased significantly in a dose-dependent manner (Fig. 2c).

ATP release on the plasma membrane and mitochondrial ATP levels in PMNs were higher in sepsis patients than in HC subjects

The MFIs of PMAP-1 and MitoAP-1 in sepsis patients were evaluated by flow cytometry within 24 h after diagnosis of sepsis (days 0–1). Both values were significantly higher than those of the HC group (Fig. 3a, b). CD11b expression on the plasma membrane was also upregulated in sepsis patients (Fig. 3c). Activated PMNs in sepsis patients appeared to cause a burst of extracellular ATP release and to increase ATP synthesis by oxidative phosphorylation in the mitochondria. The same experiments were conducted at days 3–4 as the patients’ clinical conditions improved, which revealed that the MFIs of PMAP-1 and CD11b expression decreased significantly compared to those at days 0–1 (Fig. 3a, c), whereas a high MitoAP-1 MFI was maintained (Fig. 3b). Namely, the temporal changes of ATP release and CD11b expression on the plasma membrane were similar, but ATP production showed distinct behavior in the mitochondria.

ATP release on the plasma membrane correlated with PMN activation

As described above, ATP release and CD11b expression decreased as the clinical conditions of the patients improved. Furthermore, the MFIs of PMAP-1 and CD11b expression showed a significant positive correlation in the sepsis patients (Fig. 4).

ATP release on the plasma membrane correlated with WBC counts, neutrophil counts, and CRP levels

To determine relationships between ATP release on the plasma membrane or ATP production in the mitochondria and clinical conditions (WBC counts, neutrophil counts, CRP levels, SOFA scores, APACHE II scores, and JAAM DIC scores), their correlation coefficients were investigated. The MFI of PMAP-1 was significantly positively correlated with WBC counts, neutrophil counts, and CRP levels (Fig. 5), but not with SOFA scores, APACHE II scores, and JAAM DIC scores. No correlation was found between the MFI of MitoAP-1 of PMNs and any clinical variable.

Discussion

To our knowledge, this is the first report of the visualization of mitochondrial ATP in the PMNs of sepsis patients with fluorescent chemosensors. PMNs are the major leukocytes recruited to inflamed sites in response to infection. Although PMNs play a central role in protecting the host from infection, their pathophysiological processes can also cause tissue damage in the host [1, 21]. To develop therapies that can reduce PMN-induced tissue damage, it is crucial to elucidate the mechanisms underlying the activation of PMNs.
ATP, which is well known as the “molecular unit of currency” of intracellular energy transfer, is released extracellularly by physiological stimulation and acts as a signaling molecule. Burnstock et al. [22] first reported that healthy neurons can release cellular ATP, which then serves as an intercellular messenger. Recently, neurons and many other types of cells have been shown to release ATP under physiological stimulation, including PMNs [2333]. Previously, we showed that ATP release and purinergic signaling are essential for PMN activation. Extracellular ATP is released by PANX1 hemichannels and controls the chemotaxis of PMNs through P2Y2 receptors [5, 6]. The autocrine feedback through P2Y2 receptors is essential for PMN activation [6].
Substantial effort has been dedicated to developing fluorescent chemosensors for ATP to elucidate its diverse physiological functions [810, 34, 35]. To date, a few imaging studies using genetically encoded fluorescence ATP probes have been reported for this purpose [34, 35]. These probes are posttranslationally conjugated to a protein anchored to a certain organelle through genetically encoded localization signals. Although successful, these techniques are not convenient, because they require cumbersome and time-consuming processes for protein expression and maturation. Therefore, Kurishita et al. [10] developed two new types of fluorescent chemosensors, PMAP-1 and MitoAP-1, which can spontaneously and selectively localize in a specific cellular region [10]. For example, PMAP-1 localizes to the plasma membrane and can be used to detect the extracellular release of ATP, whereas MitoAP-1 localizes to the mitochondria and senses mitochondrial ATP. Thus, these fluorescent chemosensors are highly suited for investigating the dynamics of ATP in biological events occurring in target cellular regions [10].
The finding that cell surface ATP release and CD11b expression in the HC group increased dose-dependently following fMLP stimulation is consistent with our previous finding that ATP is released by fMLP stimulation via PANX1 hemichannels [6]. In sepsis patients, extracellular ATP release in PMNs was elevated on days 0–1, corroborating our previous work in mouse sepsis models, wherein plasma ATP levels were elevated and contributed to PMN activation [7]. Collectively, these findings indicate that PMNs may be a main source of elevated circulating ATP levels. Furthermore, ATP release on the plasma membrane was correlated with CD11b expression, WBC counts, neutrophil counts, and CRP levels in sepsis patients; thus, ATP release on the plasma membrane may reflect PMN activation and could potentially serve as an activation marker for sepsis. Given that excessive activation of PMNs can damage host organs, inhibition of purinergic signaling could serve as a potential therapeutic strategy for treating sepsis. Previously, we reported that the P2 receptor antagonist suramin reduced PMN activation in a mouse CLP model [7]. Thus, suramin may potentially be used as an effective therapeutic strategy for sepsis patients. In contrast to the complications mediated by an overactive neutrophil compartment, severe systemic inflammation is a risk factor for the development of immune suppression. The circulating neutrophil pool displays a paradoxical phenotype, exhibiting characteristics of both activated and suppressed functionality. Therefore, further investigations are required to resolve this paradox of the role of PMNs in sepsis [36].
Mitochondria play an essential role in the activation of PMNs by producing ATP, which triggers the purinergic signaling processes [20]. During this process, mitochondria generate ATP and release ATP extracellularly following fMLP stimulation. However, in the HC group, the mitochondrial ATP levels in PMNs decreased with fMLP stimulation in a dose-dependent manner, demonstrating that the mitochondrial ATP was consumed in this process in healthy PMNs. Mitochondrial ATP levels in PMNs from sepsis patients were significantly higher than those of the HC group. Although further investigations are required to determine the definite cause for this difference, it is intriguing to speculate that pathways other than fMLP stimulation may be essential for ATP elevation in the mitochondria. Evaluation of mitochondrial function in various cells has been previously reported in the context of sepsis [37, 38]. For example, Adrie et al. [37] demonstrated reduced mitochondrial membrane potential in peripheral blood monocytes from patients with severe sepsis. On the other hand, Belikova et al. [38] showed peripheral blood mononuclear cells from patients with severe sepsis had a significantly higher baseline value of oxygen consumption compared with healthy volunteer cells. This report supports our study that mitochondrial ATP production in PMNs from sepsis patients was increased. Therefore, understanding the interaction among cells is essential to elucidate the mechanism of sepsis. Our method shows potential to clarify the mechanism of sepsis from the point of view of mitochondrial function.
Furthermore, although the PMAP-1 MFI on days 3–4 of sepsis decreased in parallel with CD11b downregulation, the MFI of MitoAP-1 did not change. These findings suggest that ATP serves different functions on the cell surface and in the mitochondria of PMNs in sepsis patients.

Conclusions

In conclusion, we developed a novel approach for quantifying ATP release on the cell surface and mitochondrial ATP production in PMNs with two new types of fluorescent chemosensors, which was applied to the study of sepsis patients. ATP release on the plasma membrane of PMNs was demonstrated to be a potentially valuable biomarker for sepsis. Taken together with our previous studies, our current findings suggest that PMN responses can be regulated by therapeutic strategies that target the purinergic signaling mechanisms of PMNs. Additional studies are needed to determine whether and how these purinergic signaling mechanisms can be targeted in order to best benefit sepsis patients.

Authors’ contributions

KS and YS designed and performed the experiments. KS and YS analyzed the data and prepared the manuscript. YK performed the experiments with FACS. KI supervised and carried out the experiments with confocal microscopy. HN and KI supervised the isolation of PMNs. YK, HS, and IH synthesized PMAP-1 and MitoAP-1 and supervised the usage of PMAP-1 and MitoAP-1. YI and HT conceived the idea, reviewed the manuscript, and supervised the whole project. All authors read and approved the final manuscript.

Acknowledgements

This study was supported in part by the Japan Society for the Promotion of Science (23792086, 25462830, to Y.S.), a Grant-in-Aid (S1311011) from the Foundation of Strategic Research Projects in Private Universities from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan (to K.S.), and Juntendo University Young Investigator Award 2013 (K1310, to K.S).

Competing interests

The authors declare that they have no competing interests.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://​creativecommons.​org/​licenses/​by/​4.​0/​), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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