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21.06.2016 | Molecular Imaging

Fluorescence molecular tomography of DiR-labeled mesenchymal stem cell implants for osteochondral defect repair in rabbit knees

verfasst von: Markus T. Berninger, Pouyan Mohajerani, Melanie Kimm, Stephan Masius, Xiaopeng Ma, Moritz Wildgruber, Bernhard Haller, Martina Anton, Andreas B. Imhoff, Vasilis Ntziachristos, Tobias D. Henning, Reinhard Meier

Erschienen in: European Radiology | Ausgabe 3/2017

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Abstract

Objectives

To assess labelling efficiency of rabbit mesenchymal stem cells (MSCs) using the near-infrared dye 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindotricarbocyanine iodide (DiR) and detection of labelled MSCs for osteochondral defect repair in a rabbit model using fluorescence molecular tomography–X-ray computed tomography (FMT-XCT).

Methods

MSCs were isolated from New Zealand White rabbits and labelled with DiR (1.25-20 μg/mL). Viability and induction of apoptosis were assessed by XTT- and Caspase-3/-7-testing. Chondrogenic potential was evaluated by measurement of glycosaminoglycans. Labelled cells and unlabeled controls (n = 3) underwent FMT-XCT imaging before and after chondrogenic differentiation. Osteochondral defects were created surgically in rabbit knees (n = 6). Unlabeled and labelled MSCs were implanted in fibrin-clots and imaged by FMT-XCT. Statistical analyses were performed using multiple regression models.

Results

DiR-labelling of MSCs resulted in a dose-dependent fluorescence signal on planar images in trans-illumination mode. No significant reduction in viability or induction of apoptosis was detected at concentrations below 10 μg DiR/mL (p > .05); the chondrogenic potential of MSCs was not affected (p > .05). FMT-XCT of labelled MSCs in osteochondral defects showed a significant signal of the transplant (p < .05) with additional high-resolution anatomical information about its osteochondral integration.

Conclusions

FMT-XCT allows for detection of stem cell implantation within osteochondral regeneration processes.

Key Points

• DiR-labelling of MSCs shows no toxic side effects or impairment of chondrogenesis.

• Fluorescence molecular tomography allows for detection of MSCs for osteochondral defect repair.

• FMT-XCT helps to improve evaluation of cell implantation and osteochondral regeneration processes.

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Titel: Fluorescence molecular tomography of DiR-labeled mesenchymal stem cell implants for osteochondral defect repair in rabbit knees
verfasst von: Markus T. Berninger
Pouyan Mohajerani
Melanie Kimm
Stephan Masius
Xiaopeng Ma
Moritz Wildgruber
Bernhard Haller
Martina Anton
Andreas B. Imhoff
Vasilis Ntziachristos
Tobias D. Henning
Reinhard Meier
Publikationsdatum: 21.06.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: European Radiology / Ausgabe 3/2017
Print ISSN: 0938-7994
Elektronische ISSN: 1432-1084
DOI: https://doi.org/10.1007/s00330-016-4457-5

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Fluorescence molecular tomography of DiR-labeled mesenchymal stem cell implants for osteochondral defect repair in rabbit knees

Abstract

Objectives

Methods

Results

Conclusions

Key Points

Neu im Fachgebiet Radiologie

Fünf Dinge, die im Kindernotfall besser zu unterlassen sind

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Update Radiologie

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Objectives

Methods

Results

Conclusions

Key Points

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