Follicle activation is a significant and immediate cause of follicle loss after ovarian tissue transplantation
- 03.11.2017
- Fertility Preservation
Erschienen in:
- Verfasst von
- Zohar Gavish
- Itay Spector
- Gil Peer
- Stefan Schlatt
- Joachim Wistuba
- Hadassa Roness
- Dror Meirow
- Erschienen in
- Journal of Assisted Reproduction and Genetics | Ausgabe 1/2018
Abstract
Purpose
Extensive follicle loss has been demonstrated in ovarian grafts post transplantation, reducing their productivity and lifespan. Several mechanisms for this loss have been proposed, and this study aims to clarify when and how the massive follicle loss associated with transplantation of ovarian tissue graft occurs. An understanding of the mechanisms of follicle loss will pinpoint potential new targets for optimization and improvement of this important fertility preservation technique.
Methods
Frozen-thawed marmoset (n = 15), bovine (n = 37), and human (n = 46) ovarian cortical tissue strips were transplanted subcutaneously into immunodeficient castrated male mice for 3 or 7 days. Histological (H&E, Masson’s trichrome) analysis and immunostaining (Ki-67, GDF9, cleaved caspase-3) were conducted to assess transplantation-associated follicle dynamics, with untransplanted frozen-thawed tissue serving as a negative control.
Results
Evidence of extensive primordial follicle (PMF) activation and loss was observed already 3 days post transplantation in marmoset, bovine, and human tissue grafts, compared to frozen-thawed untransplanted controls (p < 0.001). No significant additional PMF loss was observed 7 days post transplantation. Recovered grafts of all species showed markedly higher rates of proliferative activity and progression from dormant to growing follicles (Ki-67 and GDF9 staining) as well as higher growing/primordial (GF/PMF) ratio (p < 0.02) and higher collagen levels compared with untransplanted controls.
Conclusions
This multi-species study demonstrates that follicle activation plays an important role in transplantation-induced follicle loss, and that it occurs within a very short time frame after grafting. These results underline the need to prevent this activation at the time of transplantation in order to retain the maximal possible follicle reserve and extend graft lifespan.
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- Titel
- Follicle activation is a significant and immediate cause of follicle loss after ovarian tissue transplantation
- Verfasst von
-
Zohar Gavish
Itay Spector
Gil Peer
Stefan Schlatt
Joachim Wistuba
Hadassa Roness
Dror Meirow
- Publikationsdatum
- 03.11.2017
- Verlag
- Springer US
- Erschienen in
-
Journal of Assisted Reproduction and Genetics / Ausgabe 1/2018
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330 - DOI
- https://doi.org/10.1007/s10815-017-1079-z
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