nach oben

Rechtsmedizin

Erschienen in:

03.08.2016 | Forensische DNA-Analyse | CME

Analyse von Hautkontaktspuren in der forensischen Genetik unter besonderer Berücksichtigung ihrer Entstehung und Spurenentnahme

verfasst von: C. Pfeifer, E. Miltner, Prof. Dr. P. Wiegand

Erschienen in: Rechtsmedizin | Ausgabe 5/2016

Einloggen, um Zugang zu erhalten

Zusammenfassung

Hautkontaktspuren bilden in der forensischen Genetik seit Ende der 1990er Jahre einen festen Bestandteil der biologischen Spuren. Dieser Beitrag verschafft dem Leser einen Überblick über Hautkontaktspuren in der forensisch-genetischen Spurenanalyse. Definitionen werden behandelt und mögliche Quellen von Hautkontaktspuren diskutiert. Entsteht eine DNA-Spur durch einen Hautkontakt, hängt das Analyseergebnis von einer Reihe an Einflussfaktoren ab: Parameter für Deposition, Haltbarkeit und Rückgewinnung werden beleuchtet. Das Verständnis der Grundlagen ist die Voraussetzung für eine geeignete Spurenentnahme, die entsprechend der Fragestellung, der Deliktart und der Oberflächenart ausgewählt werden sollte. Entnahmemethoden werden vorgestellt; Kriterien für die Spurenauswahl sowie zeitliche und räumliche Strategien werden diskutiert.

Van Oorschot RAH, Jones MK (1997) DNA fingerprints from fingerprints. Nature 387:767 CrossRefPubMed

Wiegand P, Kleiber M (1997) DNA typing of epithelial cells after strangulation. Int J Legal Med 110:181–183 CrossRefPubMed

Hammer U, Bulnheim U, Karstädt G, Meissner D, Wegener R (1997) Zur DNA-Typisierung übertragener Hautzellen nach körperlicher Gewalt. Rechtsmedizin 7:180–183 CrossRef

Castella V, Mangin P (2008) DNA profiling success and relevance of 1739 contact stains from caseworks. Forensic Sci Int Genet Suppl Ser 1:405–407 CrossRef

Gill P, Fereday L, Morling N, Schneider PM (2006) The evolution of DNA databases – Recommendations for new European STR loci. Forensic Sci Int 156:242–244 CrossRefPubMed

Welch LA, Gill P, Phillips C, Ansell R, Morling N, Parson W, Palo JU, Bastisch I (2012) European Network of Forensic Science Institutes (ENFSI): Evaluation of new commercial STR multiplexes that include the European Standard Set (ESS) of markers. Forensic Sci Int Genet 6:819–826 CrossRefPubMed

Meakin G, Jamieson A (2013) DNA transfer: Review and implications for casework. Forensic Sci Int Genet 7:434–443 CrossRefPubMed

Wickenheiser RA (2002) Trace DNA: a review, discussion of theory, and application of the transfer of trace quantities of DNA through skin contact. J Forensic Sci 47:442–450 PubMed

Van Oorschot RAH, Ballantyne K, Mitchell R (2010) Forensic trace DNA: a review. Investig Genet 1:1–17 CrossRef

10.

Gill P (2014) Definitions: Contamination and interpretation. In: Gill P (Hrsg) Misleading DNA evidence – Reasons for miscarriages of justice. Academic Press, London, S 1–19 CrossRef

11.

Aditya S, Sharma AK, Bhattacharyya CN, Chaudhuri K (2011) Generating STR profile from Touch DNA. J Forensic Leg Med 18:295–298 CrossRefPubMed

12.

Ryan SR (2012) Touch DNA analysis: Using the literature to help answer some common questions. http://www.forensicmag.com/articles/2012/06/touch-dna-analysis-using-literature-help-answer-some-common-questions#.Uimi7395FxU. Zugegriffen: 20. Nov. 2015

13.

Quinones I, Daniel B (2012) Cell free DNA as a component of forensic evidence recovered from touched surfaces. Forensic Sci Int Genet 6:26–30 CrossRefPubMed

14.

Balogh MK, Burger J, Bender K, Schneider PM, Alt KW (2003) STR genotyping and mtDNA sequencing of latent fingerprint on paper. Forensic Sci Int 137:188–195 CrossRefPubMed

15.

Jiang X (2009) One method of collecting fallen off epithelial cell. Forensic Sci Int Genet Suppl Ser 2:193 CrossRef

16.

Lazar AJF, Wang W‑L (2004) The skin. In: Kumar V (Hrsg) Robbins basic pathology. Saunders Elsevier, Philadelphia, S 789–807

17.

Moll I (2005) Mikroskopische Struktur und Differenzierung der Haut. In: Moll I (Hrsg) Duale Reihe Dermatologie. Thieme, Stuttgart, S 3–13

18.

Teraki Y, Shiohara T (1999) Apoptosis and the skin. Eur J Dermatol 9:413–425 (quiz 426) PubMed

19.

Alessandrini F, Cecati M, Pesaresi M, Turchi C, Carle F, Tagliabracci A (2003) Fingerprints as evidence for a genetic profile: morphological study on fingerprints and analysis of exogenous and individual factors affecting DNA typing. J Forensic Sci 48:586–592 CrossRefPubMed

20.

Wiegand P, Truebner K, Kleiber M (2000) STR typing of biological stains on strangulation tools. In: Sensabaugh GF, Lincoln PJ, Olaisen B (Hrsg) Progress in forensic genetics. Elsevier Science, Amsterdam, S 508–510

21.

Kita T, Yamaguchi H, Yokoyama M, Tanaka T, Tanaka N (2008) Morphological study of fragmented DNA on touched objects. Forensic Sci Int Genet 3:32–36 CrossRefPubMed

22.

Sewell J, Quinones I, Ames C, Multaney B, Curtis S, Seeboruth H, Moore S, Daniel B (2008) Recovery of DNA and fingerprints from touched documents. Forensic Sci Int Genet 2:281–285 CrossRefPubMed

23.

Zoppis S, Muciaccia B, D’Alessio A, Ziparo E, Vecchiotti C, Filippini A (2014) DNA fingerprinting secondary transfer from different skin areas: morphological and genetic studies. Forensic Sci Int Genet 11:137–143 CrossRefPubMed

24.

Lowe A, Murray C, Whitaker J, Tully G, Gill P (2002) The propensity of individuals to deposit DNA and secondary transfer of low level DNA from individuals to inert surfaces. Forensic Sci Int 129:25–34 CrossRefPubMed

25.

Phipps M, Petricevic S (2007) The tendency of individuals to transfer DNA to handled items. Forensic Sci Int 168:162–168 CrossRefPubMed

26.

Vandewoestyne M, Van Hoofstat D, Franssen A, Van Nieuwerburgh F, Deforce D (2013) Presence and potential of cell free DNA in different types of forensic samples. Forensic Sci Int Genet 7:316–320 CrossRefPubMed

27.

Linacre A, Pekarek V, Swaran YC, Tobe SS (2010) Generation of DNA profiles from fabrics without DNA extraction. Forensic Sci Int Genet 4:137–141 CrossRefPubMed

28.

Bright J‑A, Petricevic SF (2004) Recovery of trace DNA and its application to DNA profiling of shoe insoles. Forensic Sci Int 145:7–12 CrossRefPubMed

29.

Djuric M, Varljen T, Stanojevic A, Stojkovic O (2008) DNA typing from handled items. Forensic Sci Int Genet Suppl Ser 1:411–412 CrossRef

30.

Farmen RK, Jaghø R, Cortez P, Frøyland ES (2008) Assessment of individual shedder status and implication for secondary DNA transfer. Forensic Sci Int Genet Suppl Ser 1:415–417 CrossRef

31.

Goray M, Mitchell RJ, Van Oorschot RAH (2010) Investigation of secondary DNA transfer of skin cells under controlled test conditions. Leg Med 12:117–120 CrossRef

32.

Kamphausen T, Schadendorf D, Wurmb-Schwark N, Bajanowski T, Poetsch M (2012) Good shedder or bad shedder – the influence of skin diseases on forensic DNA analysis from epithelial abrasions. Int J Legal Med 126:179–183 CrossRefPubMed

33.

Poetsch M, Bajanowski T, Kamphausen T (2013) Influence of an individual’s age on the amount and interpretability of DNA left on touched items. Int J Legal Med 127:1093–1096 CrossRefPubMed

34.

Daly DJ, Murphy C, McDermott SD (2012) The transfer of touch DNA from hands to glass, fabric and wood. Forensic Sci Int Genet 6:41–46 CrossRefPubMed

35.

Farmen RK, Cortez P, Frøyland ES (2008) Spermatozoa recovered on laundered clothing. Forensic Sci Int Genet Suppl Ser 1:418–420 CrossRef

36.

Balogh MK, Burger J, Bender K, Schneider PM, Alt KW (2003) Fingerprints from fingerprints. In: International congress series. Elsevier, Amsterdam, S 953–957

37.

Graham EAM, Rutty GN (2008) Investigation into „normal“ background DNA on adult necks: Implications for DNA profiling of manual strangulation victims. J Forensic Sci 53:1074–1082 PubMed

38.

Van Oorschot RAH, Phelan DG, Furlong S, Scarfo GM, Holding NL, Cummins MJ (2003) Are you collecting all the available DNA from touched objects? Int Congr Ser 1239:803–807 CrossRef

39.

Herber B, Herold K (1998) DNA typing of human dandruff. J Forensic Sci 43:648–656 CrossRefPubMed

40.

Herz E (2005) Präkanzerosen. In: Moll I (Hrsg) Duale Reihe Dermatologie. Thieme, Stuttgart, S 306–312

41.

Tsianakas A, Jung EG (2005) Diagnostik und Differenzialdiagnose. In: Moll I (Hrsg) Duale Reihe Dermatologie. Thieme, Stuttgart, S 467–469

42.

Knußmann-Hartig E (2015) Histologische Verfahren. In: Moll I (Hrsg) Duale Reihe Dermatologie. Thieme, Stuttgart, S 43–47

43.

Weßbecher R, Voigtländer V (2005) Typ-IV-Reaktion vom Spättyp, Ekzemkrankheiten. In: Moll I (Hrsg) Duale Reihe Dermatologie. Thieme, Stuttgart, S 129–142

44.

Bayerl C, Voigtländer V (2005) Atopische Dermatitis. In: Moll I (Hrsg) Duale Reihe Dermatologie. Thieme, Stuttgart, S 158–165

45.

Lorente M, Entrala C, Lorente JA, Alvarez JC, Villanueva E, Budowle B (1998) Dandruff as a potential source of DNA in forensic casework. J Forensic Sci 43:901–902 CrossRefPubMed

46.

Kikuchi T, Horii I, Sakamoto T, Nakayama Y, Tagami H (1989) Demonstration of neutrophil chemotactic anaphylatoxins in human dandruff. Arch Dermatol Res 281:482–486 CrossRefPubMed

47.

Sommerer T (2011) Selektive Hautschuppenanalyse – Strategien zur Gewinnung von Individual-STR-Profilen aus Hautepithelzellen. Dissertation. Universität Ulm, Ulm

48.

Szkuta B, Harvey ML, Ballantyne KN, Van Oorschot RAH (2015) DNA transfer by examination tools – a risk for forensic casework? Forensic Sci Int Genet 16:246–254 CrossRefPubMed

49.

Fonneløp AE, Egeland T, Gill P (2015) Secondary and subsequent DNA transfer during criminal investigation. Forensic Sci Int Genet 17:155–162 CrossRefPubMed

50.

Rutty GN (2002) An investigation into the transference and survivability of human DNA following simulated manual strangulation with consideration of the problem of third party contamination. Int J Legal Med 116:170–173 CrossRefPubMed

51.

De Bruin KG, Verheij SM, Veenhoven M, Sijen T (2012) Comparison of stubbing and the double swab method for collecting offender epithelial material from a victim’s skin. Forensic Sci Int Genet 6:219–223 CrossRefPubMed

52.

Raymond JJ, Van Oorschot RAH, Walsh SJ, Roux C, Gunn PR (2009) Trace DNA and street robbery: A criminalistic approach to DNA evidence. Forensic Sci Int Genet Suppl Ser 2:544–546 CrossRef

53.

Goray M, Van Oorschot RAH (2015) The complexities of DNA transfer during a social setting. Leg Med 17:82–91 CrossRef

54.

Stouder SL, Reubush KJ, Hobson DL, Smith JL (2001) Trace evidence scrapings: A valuable source of DNA? http://www.fbi.gov/about-us/lab/forensic-science-communications/fsc/oct2001/stouder.htm. Zugegriffen: 18. Dez. 2015

55.

Van Oorschot RAH, Glavich G, Mitchell RJ (2014) Persistence of DNA deposited by the original user on objects after subsequent use by a second person. Forensic Sci Int Genet 8:219–225 CrossRefPubMed

56.

Matte M, Williams L, Frappier R, Newman J (2012) Prevalence and persistence of foreign DNA beneath fingernails. Forensic Sci Int Genet 6:236–243 CrossRefPubMed

57.

Wiegand P, Bajanowski T, Brinkmann B (1993) DNA typing of debris from fingernails. Int J Legal Med 106:81–83 CrossRefPubMed

58.

Kettner M, Cappel-Hoffmann S, Makuch D, Schmidt P, Ramsthaler F (2015) IPV – bridging the juridical gap between scratches and DNA detection under fingernails of cohabitating partners. Forensic Sci Int Genet 14:110–115 CrossRefPubMed

59.

Malsom S, Flanagan N, McAlister C, Dixon L (2009) The prevalence of mixed DNA profiles in fingernail samples taken from couples who co-habit using autosomal and Y‑STRs. Forensic Sci Int Genet 3:57–62 CrossRefPubMed

60.

Raymond JJ, Van Oorschot RAH, Gunn PR, Walsh SJ, Roux C (2009) Trace evidence characteristics of DNA: A preliminary investigation of the persistence of DNA at crime scenes. Forensic Sci Int Genet 4:26–33 CrossRefPubMed

61.

McDonald A, Jones E, Lewis J, O’Rourke P (2015) Y‑STR analysis of digital and/or penile penetration cases with no detected spermatozoa. Forensic Sci Int Genet 15:84–89 CrossRefPubMed

62.

Schneider H, Sommerer T, Rand S, Wiegand P (2011) Hot flakes in cold cases. Int J Legal Med 125:543–548 CrossRefPubMed

63.

Goray M, Van Oorschot RAH, Mitchell JR (2012) DNA transfer within forensic exhibit packaging: Potential for DNA loss and relocation. Forensic Sci Int Genet 6:158–166 CrossRefPubMed

64.

Rudin N, Inman K (2010) How low can you go? Should you just say no? https://www.cacnews.org/news/3rdq10.pdf. Zugegriffen: 18. Dez. 2015

65.

Schulz MM, Wehner HD, Reichert W, Graw M (2004) Ninhydrin-dyed latent fingerprints as a DNA source in a murder case. J Clin Forensic Med 11:202–204 CrossRefPubMed

66.

Plaza DT, Mealy JL, Lane JN, Parsons MN, Bathrick AS, Slack DP (2015) ESDA®-Lite collection of DNA from latent fingerprints on documents. Forensic Sci Int Genet 16:8–12 CrossRefPubMed

67.

Verdon TJ, Mitchell RJ, Van Oorschot RAH (2014) Swabs as DNA collection devices for sampling different biological materials from different substrates. J Forensic Sci 59:1080–1089 CrossRefPubMed

68.

Sweet D, Lorente M, Lorente JA, Valenzuela A, Villanueva E (1997) An improved method to recover saliva from human skin: the double swab technique. J Forensic Sci 42:320–322 PubMed

69.

Pang BCM, Cheung BKK (2007) Double swab technique for collecting touched evidence. Leg Med 9:181–184 CrossRef

70.

Hansson O, Finnebraaten M, Heitmann IK, Ramse M, Bouzga M (2009) Trace DNA collection – Performance of minitape and three different swabs. Forensic Sci Int Genet Suppl Ser 2:189–190 CrossRef

71.

Verdon TJ, Mitchell RJ, Van Oorschot RAH (2014) Evaluation of tapelifting as a collection method for touch DNA. Forensic Sci Int Genet 8:179–186 CrossRefPubMed

72.

Schulz MM, Reichert W (2002) Archived or directly swabbed latent fingerprints as a DNA source for STR typing. Forensic Sci Int 127:128–130 CrossRefPubMed

73.

Zamir A, Springer, Glattstein B (2000) Fingerprints and DNA: STR typing of DNA extracted from adhesive tape after processing for fingerprints. J Forensic Sci 45:687–688 PubMed

74.

May R, Thomson J (2009) Optimisation of cellular DNA recovery from tape-lifts. Forensic Sci Int Genet Suppl Ser 2:191–192 CrossRef

75.

Verdon TJ, Ballantyne KN, Mitchell RJ, Van Oorschot RAH (2011) The Pinpoint DNA Isolation System as a novel DNA sampling method in forensic biology. Forensic Sci Int Genet Suppl Ser 3:e91–e92 CrossRef

76.

Hall D, Fairley M (2004) A single approach to the recovery of DNA and firearm discharge residue evidence. Sci Justice 44:15–19 CrossRefPubMed

77.

Petricevic SF, Bright J‑A, Cockerton SL (2006) DNA profiling of trace DNA recovered from bedding. Forensic Sci Int 159:21–26 CrossRefPubMed

78.

Verdon TJ, Mitchell RJ, Van Oorschot RAH (2015) Preliminary investigation of differential tapelifting for sampling forensically relevant layered deposits. Leg Med 17:553–559 CrossRef

79.

Anslinger K, Bayer B, Mack B, Eisenmenger W (2007) Sex-specific fluorescent labelling of cells for laser microdissection and DNA profiling. Int J Legal Med 121:54–56 CrossRefPubMed

80.

Vandewoestyne M, Van Hoofstat D, Van Nieuwerburgh F, Deforce D (2009) Automatic detection of spermatozoa for laser capture microdissection. Int J Legal Med 123:169–175 CrossRefPubMed

81.

Verdon TJ, Mitchell RJ, Chen W, Xiao K, Van Oorschot RAH (2015) FACS separation of non-compromised forensically relevant biological mixtures. Forensic Sci Int Genet 14:194–200 CrossRefPubMed

82.

Fontana F, Rapone C, Bregola G, Aversa R, Lanzellotto R, Medoro G, Manaresi N, Vittorioso P, Berti A (2015) DEPArray digital sorting of biological mixtures achieves isolation of 100 % pure cell popoulations for clear-cut genetic analysis. 26th congress of the International society for forensic genetics. http://www.siliconbiosystems.com/request-isfg-2015-poster. Zugegriffen: 18. Dez. 2015

83.

Farash K, Hanson EK, Ballantyne J (2015) Enhanced genetic analysis of single human bioparticles recovered by simplified micromanipulation from forensic „Touch DNA“ evidence. J Vis Exp 97:e52612. doi:10.3791/52612

Titel: Analyse von Hautkontaktspuren in der forensischen Genetik unter besonderer Berücksichtigung ihrer Entstehung und Spurenentnahme
verfasst von: C. Pfeifer
E. Miltner
Prof. Dr. P. Wiegand
Publikationsdatum: 03.08.2016
Verlag: Springer Berlin Heidelberg
Schlagwörter: Forensische DNA-Analyse
Squama
Erschienen in: Rechtsmedizin / Ausgabe 5/2016
Print ISSN: 0937-9819
Elektronische ISSN: 1434-5196
DOI: https://doi.org/10.1007/s00194-016-0110-5

Neu im Fachgebiet Rechtsmedizin

24.02.2023 | Neuroendokrine Tumoren | CME

Hauptrubriken

Service

Fachgebiete

Themen

Sammlungen

Springer Medizin

Analyse von Hautkontaktspuren in der forensischen Genetik unter besonderer Berücksichtigung ihrer Entstehung und Spurenentnahme

Zusammenfassung

Neu im Fachgebiet Rechtsmedizin

Neue WHO-Klassifikation 2022: Harnblasenkarzinom

Ultrastruktur pathologischer Ablagerungen und Zellinklusionen

Neuropathologie II: Erkrankungen des zentralen und peripheren Nervensystems

B3-Läsionen der Mamma: Histologische, klinische und epidemiologische Aspekte

Hauptrubriken

Service

Fachgebiete

Themen

Sammlungen

Springer Medizin

Zusammenfassung

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 5/2016

Auf der falschen Fährte

Identifizierung einer skelettierten „Kofferleiche“ aus Berlin

Biomechanik des Stampftritts gegen den behelmten Schädel

Rechtsmedizinische Aspekte der Lichtenberg-Figuren nach Blitzschlag

Mitteilungen der Deutschen Gesellschaft für Rechtsmedizin

Dehydratation und Alkoholwirkung als kombinierte Ursachen eines akuten Delirs

Neu im Fachgebiet Rechtsmedizin

Neue WHO-Klassifikation 2022: Harnblasenkarzinom

Ultrastruktur pathologischer Ablagerungen und Zellinklusionen

Neuropathologie II: Erkrankungen des zentralen und peripheren Nervensystems

B3-Läsionen der Mamma: Histologische, klinische und epidemiologische Aspekte