nach oben

European Journal of Clinical Microbiology & Infectious Diseases

Erschienen in:

08.07.2020 | Brief Report

Fourier transform infrared (FT-IR) spectroscopy typing: a real-time analysis of an outbreak by carbapenem-resistant Klebsiella pneumoniae

verfasst von: Liliana Silva, Carla Rodrigues, Agostinho Lira, Mariana Leão, Margarida Mota, Paulo Lopes, Ângela Novais, Luísa Peixe

Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases | Ausgabe 12/2020

Einloggen, um Zugang zu erhalten

Abstract

The spread of multidrug-resistant (MDR) Klebsiella pneumoniae in the nosocomial setting represents a big challenge to infection control teams. We have recently developed a simple spectroscopic–based method with excellent accuracy, turnaround time and cost-effectiveness (Rodrigues et al. mSystems 2020) for bacterial typing. Here, we applied our method in a real clinical context to support early identification of an outbreak involving KPC-3-producing K. pneumoniae ST147 isolates. Our results further support that attenuated total reflectance Fourier transform infrared (FT-IR) spectroscopy can provide enough information to support early and adequate infection control measures and therapeutic choices in the context of nosocomial outbreaks and hospital surveillance.

Nur mit Berechtigung zugänglich

Logan LK, Weinstein RA (2017) The epidemiology of carbapenem-resistant Enterobacteriaceae: the impact and evolution of a global menace. J Infect Dis 215(suppl_1):S28–S36CrossRef

Rossen JWA, Friedrich AW, Moran-Gilad J (2018) ESCMID Study Group for Genomic and Molecular Diagnostics (ESGMD). Practical issues in implementing whole-genome-sequencing in routine diagnostic microbiology. Clin Microbiol Infect 24(4):355–360CrossRef

Rodrigues C, Sousa C, Lopes JA, Novais Â, Peixe L (2020) One the front line of Klebsiella pneumoniae surface structures understanding: establishment of Fourier Transform Infrared (FT-IR) spectroscopy as a capsule typing method. mSystems 5(2):e00386–e00319CrossRef

Novais Â, Freitas AR, Rodrigues C, Peixe L (2019) Fourier transform infrared spectroscopy: unlocking fundamentals and prospects for bacterial strain typing. Eur J Clin Microbiol Infect Dis 38(3):427–448CrossRef

Brisse S, Passet V, Haugaard AB, Babosan A, Kassis-Chikhani N, Struve C et al (2013) wzi Gene sequencing, a rapid method for determination of capsular type for Klebsiella strains. J Clin Microbiol 51(12):4073–4078CrossRef

Pires J, Novais Â, Peixe L (2013) Blue-Carba, an easy biochemical test for detection of diverse carbapenemase producers directly from bacterial cultures. J Clin Microbiol 51(12):4281–4283CrossRef

Rodrigues C, Machado E, Ramos H, Peixe L, Novais Â (2014) Expansion of ESBL-producing Klebsiella pneumoniae in hospitalized patients: a successful story of international clones (ST15, ST147, ST336) and epidemic plasmids (IncR, IncFIIK). Int J Med Microbiol 304(8):1100–1108CrossRef

Rodrigues C, Bavlovič J, Machado E, Amorim J, Peixe L, Novais Â (2016) KPC-3-producing Klebsiella pneumoniae in Portugal linked to previously circulating non-CG258 lineages and uncommon genetic platforms (Tn4401d-IncFIA and Tn4401d-IncN). Front Microbiol 28(7):1000

(2016) Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing. In: CLSI supplement M100-S, 26th edn. Clinical and Laboratory Standards Institute, Wayne

Titel: Fourier transform infrared (FT-IR) spectroscopy typing: a real-time analysis of an outbreak by carbapenem-resistant Klebsiella pneumoniae
verfasst von: Liliana Silva
Carla Rodrigues
Agostinho Lira
Mariana Leão
Margarida Mota
Paulo Lopes
Ângela Novais
Luísa Peixe
Publikationsdatum: 08.07.2020
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Clinical Microbiology & Infectious Diseases / Ausgabe 12/2020
Print ISSN: 0934-9723
Elektronische ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-020-03956-y

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

25.04.2024 | Hypotonie | Nachrichten

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Fourier transform infrared (FT-IR) spectroscopy typing: a real-time analysis of an outbreak by carbapenem-resistant Klebsiella pneumoniae

Abstract

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Update Innere Medizin

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 12/2020

Epidemiological analysis of Group A Streptococcus infections in a hospital in Beijing, China

Leprosy surveillance study in a highly endemic Brazilian area using leprosy specific serologic tests and IFNγ whole blood assay

Streptococcus agalactiae in pregnant women: serotype and antimicrobial susceptibility patterns over five years in Eastern Sicily (Italy)

Impact of cerebrospinal fluid syndromic testing in the management of children with suspected central nervous system infection

Epidemiology of Haemophilus influenzae in the Republic of Ireland, 2010–2018

Evaluation of three immunochromatographic tests for rapid detection of antibodies against SARS-CoV-2

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Update Innere Medizin