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Journal of Assisted Reproduction and Genetics
Erschienen in: Journal of Assisted Reproduction and Genetics 12/2016

01.09.2016 | Gamete Biology

Freeze/thaw stress induces organelle remodeling and membrane recycling in cryopreserved human mature oocytes

verfasst von: Stefania Annarita Nottola, Elena Albani, Giovanni Coticchio, Maria Grazia Palmerini, Caterina Lorenzo, Giulia Scaravelli, Andrea Borini, Paolo Emanuele Levi-Setti, Guido Macchiarelli

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 12/2016

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Abstract

Purpose

Our aim was to evaluate the ultrastructure of human metaphase II oocytes subjected to slow freezing and fixed after thawing at different intervals during post-thaw rehydration.

Methods

Samples were studied by light and transmission electron microscopy.

Results

We found that vacuolization was present in all cryopreserved oocytes, reaching a maximum in the intermediate stage of rehydration. Mitochondria-smooth endoplasmic reticulum (M-SER) aggregates decreased following thawing, particularly in the first and intermediate stages of rehydration, whereas mitochondria-vesicle (MV) complexes augmented in the same stages. At the end of rehydration, vacuoles and MV complexes both diminished and M-SER aggregates increased again. Cortical granules (CGs) were scarce in all cryopreserved oocytes, gradually diminishing as rehydration progressed.

Conclusions

This study also shows that such a membrane remodeling is mainly represented by a dynamic process of transition between M-SER aggregates and MV complexes, both able of transforming into each other. Vacuoles and CG membranes may take part in the membrane recycling mechanism.
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Metadaten
Titel
Freeze/thaw stress induces organelle remodeling and membrane recycling in cryopreserved human mature oocytes
verfasst von
Stefania Annarita Nottola
Elena Albani
Giovanni Coticchio
Maria Grazia Palmerini
Caterina Lorenzo
Giulia Scaravelli
Andrea Borini
Paolo Emanuele Levi-Setti
Guido Macchiarelli
Publikationsdatum
01.09.2016
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 12/2016
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-016-0798-x

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